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Table 4 Reproducibility (inter-assay) and repeatability (intra-assay) variation values of the TaqMan-qPCR assay for tenfold serial dilutions of A. capra. The samples were researched three times in five independent assays (n = 15)

From: Application of LAMP and TaqMan qPCR for the rapid diagnosis of Anaplasma Capra (an emerging tick-borne zoonotic pathogen) and comparison with Nested-PCR

Log10 copy number

Mean Cq

Inter-run SD

Intra-run SD

Total SD

Total CV (%)

1010

8.149

0.0098

0.068

0.069

0.85

109

13.033

0.419

0.154

0.446

3.43

108

15.420

0.404

0.133

0.420

2.72

107

21.100

0.260

0.238

0.353

1.67

106

24.986

0.240

0.202

0.314

1.26

105

26.500

0.544

0.145

0.563

2.12

104

31.627

0.330

0.179

0.374

1.18

103

32.733

0.678

0.322

0.751

2.29

102

39.013

0.196

0.695

0.722

1.85

101

39.800

0.218

0.128

0.253

0.64

100

40.000

0.000

0.000

0.000

0.00

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