Fig. 5

Detection limit of molecular methods. A: groEL-nested PCR (positive amplicon was up to dilution of 10− 6), B: groEL-TaqMan qPCR (sigmoidal waves were present up to dilution of 10− 7), C: groEL-colorimetric LAMP (color shift was observed up to a dilution of 10− 8), D: groEL-conventional LAMP (ladder-like amplicons were seen up to a dilution of 10− 8)