Fig. 4

Specificity assays of molecular methods. A: (1) A. capra, (2) A. ovis, 3. A. bovis, 4. A. marginale, 5. A. centrale, 6. (A) phagocytophilum like-1 and like-2 mix, 7. Anaplasma negative, 8. (B) bigemina, 9. B. major, 10. B. occultans, 11. T. annulata, 12. T. orientalis, 13. Theileria sp. MK., 14. T. ovis, 15. Theileria sp. OT3, 16. T. equi, 17. Theileria/Babesia negative. B: The colorimetric LAMP gave positive results (color change) for A. capra (1), but negative results for other Anaplasma/Theileria/Babesia samples. C: Conventional LAMP gave positive results (ladder-like amplicons) for A. capra (1), but negative results for other Anaplasma/Theileria/Babesia samples. D: The TaqMan qPCR gave positive results (sigmoidal wave) for A. capra (1) and negative results for other Anaplasma/Theileria/Babesia species