Fig. 3

The optimization of annealing temperatures for the TaqMan qPCR using A. capra positive and negative samples at different temperatures. A: Annealing temperature: 57 °C, B: Annealing temperature: 58 °C, C: Annealing temperature: 59 °C, D: Annealing temperature: 60 °C. A sigmoidal wave was observed in the positive sample