Abstract
Mycotoxins, produced by fungi, can contaminate fish food and harm their health. Probiotics enhance immune balance and primarily function in the animal intestine. This study aimed to assess aflatoxin’s impact on Piaractus mesopotamicus and explore probiotic-based additive (PBA) benefits in mitigating these effects, focusing on antioxidant activity, biochemical indices, and hepatic histopathology. Two experiments were conducted using P. mesopotamicus fry. The first experimental assay tested various levels of aflatoxin B1 (0.0, 25.0, 50.0, 100.0, 200.0, and 400.0 µg kg−1) over a 10-day period. The second experimental assay examined the efficacy of the probiotic (supplemented at 0.20%) in diets with different levels of aflatoxin B1 (0.0, 25.0, and 400.0 µg kg−1) for 15 days. At the end of each assay, the fish underwent a 24-hour fasting period, and the survival rate was recorded. Six liver specimens from each treatment group were randomly selected for metabolic indicator assays, including superoxide dismutase, catalase, alanine aminotransferase, aspartate aminotransferase, and albumin. Additionally, histopathological analysis was performed on six specimens. The initial study discovered that inclusion rates above 25.0 µg kg−1 resulted in decreased activity of AST (aspartate aminotransferase), ALT (alanine aminotransferase), ALB (albumin), CAT (catalase), and SOD (superoxide dismutase), accompanied by liver histopathological lesions. In the second study, the inclusion of PBA in diets contaminated with AFB1 improved the activity of AST and ALT up to 25.0 µg kg−1 of AFB1, with no histopathological lesions observed. The study demonstrated the hepatoprotective effects of PBA in diets contaminated with AFB1. The enzyme activity and hepatic histopathology were maintained, indicating a reduction in damage caused by high concentrations of AFB1 (400.0 µg kg−1 of AFB1). The adverse effects of AFB1 on biochemical and histopathological parameters were observed from 25.0 µg kg−1 onwards. Notably, PBA supplementation enhanced enzymatic activity at a concentration of 25 µg kg−1 of AFB1 and mitigated the effects at 400.0 µg kg−1 of AFB1. The use of PBAs in pacu diets is highly recommended as they effectively neutralize the toxic effects of AFB1 when added to diets containing 25.0 µg kg−1 AFB1. Dietary inclusion of aflatoxin B1 at a concentration of 25.0 µg kg−1 adversely affects the liver of Piaractus mesopotamicus (Pacu). However, the addition of a probiotic-based additive (PBA) to the diets containing this concentration of aflatoxin neutralized its toxic effects. Therefore, the study recommends the use of PBAs in Pacu diets to mitigate the adverse effects of aflatoxin contamination.
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The authors would like to thank the CAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior for Henrique M. Ziemniczak’s scholarship and the Universidade Federal da Grande Dourados for providing structure and technical support.
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Scholarship received by Henrique M. Ziemniczak from CAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior.
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Annye C. V. Ferreira -Writing, review & editing; Antonio Cesar Godoy - Writing, review & editing; Claucia A. Honorato - Conceptualization, Investigation, Methodology, Writing, review & editing; Dacley H. Neu - Conceptualization, Investigation, Methodology, Writing, review & editing; Henrique Momo Ziemniczal - Investigation, Methodology, Writing, review & editing; Jeferson R. Gandra - Writing, review & editing; Klaus C. Saturnino - Writing, review & editing; Leticia Fantini-Hoag - Writing, review & editing; Ulisses de Padua Pereira - Conceptualization, Investigation, Writing, review & editing; Welinton V. da Silva - Methodology, Writing, review & editing
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Godoy, A.C., Ziemniczak, H.M., Fantini-Hoag, L. et al. The effects of probiotic-based additives on aflatoxin intoxication in Piaractus mesopotamicus: a study of liver histology and metabolic performance. Vet Res Commun (2024). https://doi.org/10.1007/s11259-024-10409-w
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DOI: https://doi.org/10.1007/s11259-024-10409-w