Abstract
Porcine parvovirus 6 (PPV6) was first identified in aborted swine fetuses in China in 2014. Since its identification, an increased number of PPV6 cases have been reported in many countries with developed pig breeding. In this study, the first identification of porcine parvovirus 6 in Russia, its phylogenetic analysis, and its characterization in vitro are reported. During the investigation, 521 serum samples collected from pigs of different ages from seven regions of the Russian Federation were tested. In four regions, the DNA of the virus was detected. The overall prevalence of porcine parvovirus 6 in Russia was 9.4%. Fattening pigs were the group with the most frequent detection of the virus genome. Phylogenetic analysis of the Russian isolate detected in a domestic boar indicated high homology with strains from Spain. In vitro studies revealed that the most promising cell cultures for PPV6 isolation are SPEV and SK. Our results demonstrated that PPV6 induced typical apoptotic features in cells, including DNA fragmentation, chromatin margination, nuclear condensation, pyknosis of nuclei, symplast formation, and various pathological mitoses.
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Data availability
The capsid protein of PPV6 of the Kem8 isolate was deposited in GenBank under accession number OR001791.
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The study was carried out within the framework of State Assignment No. FGUG-2022–00010 of the Federal State Budget Scientific Institution “Federal Scientific Center VIEV”, Moscow, Russia.
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Conceptualization, AK, AM, NK and AG; AK, AM, and AG conceived and designed the study. AK and AM conducted the experiments. AK, AM, NK and AG analyzed the data and wrote the original draft. All authors have reviewed and approved the final manuscript.
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The samples used in this study originated from pig submissions to the Laboratory of Biochemistry and Molecular Biology of the Federal State Budget Scientific Institution “Federal Scientific Center VIEV” for diagnostic studies. The protocol for this study was approved by the Ethical and Animal Welfare Committee of the Federal State Budget Scientific Institution “Federal Scientific Center VIEV”, (Moscow, Russia).
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Komina, A., Anoyatbekova, A., Krasnikov, N. et al. Identification and in vitro characterization of a novel porcine parvovirus 6 in Russia. Vet Res Commun (2023). https://doi.org/10.1007/s11259-023-10226-7
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DOI: https://doi.org/10.1007/s11259-023-10226-7