Abstract
Desirable characteristics of Staphylococcus sp., Streptococcus sp., Bacillus sp., Klebsiella sp., Escherichia coli, and Pseudomonas pseudoalcaligenes isolated from the trachea of healthy turkeys were evaluated as probiotic candidates in the search for new alternatives to solve antimicrobial resistance issues in poultry. In current study phenotypic and genotypic capacity to produce bacteriocin-like substances, efficacy to inhibit the growth of avian pathogens, susceptibility to antimicrobials of bacteria isolated from the respiratory microbiota of healthy turkeys, and the presence of virulence-associated genes (VAGs) predictors of Avian Pathogenic Escherichia coli (APEC) were evaluated. Nine E. coli and one Klebsiella sp. strains produced bacteriocin-like substances, and all harbored the cvaA gene. Some strains also showed antagonistic activity against APEC. Multidrug-resistant profile was found in 54% of the strains. Six strains of bacteriocin-like substances producing E. coli also harbored 3–5 VAGs. The study showed that two bacterial genuses (Klebsiella sp. and E. coli) present desirable probiotic characteristics. Our results identified strains with potential for poultry’s respiratory probiotic.
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Benito Guimarães de Brito and Kelly Cristina Tagliari de Brito conceived the project and supervised the study. Michele Trintinaglia, Luís Eduardo de Souza Gazal, and Victor Dellevedove Cruz performer experiments. Luciana Kazue Otutumi, Renata Katsuko Takayama Kobayashi, and Gerson Nakazato performed formal analysis. Michele Trintinaglia, Kelly Cristina Tagliari de Brito, Renata Katsuko Takayama Kobayashi, Gerson Nakazato, Lu?s Eduardo de Souza Gazal, and Benito Guimarães de Brito wrote previous versions of the manuscript. All authors read and approved the final manuscript.
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Kelly Cristina Tagliari de Brito and Benito Guimar?es de Brito report that they received by National Council for Scientific and Technological Development funding grants.
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Trintinaglia, M., de Brito, K.C.T., Kobayashi, R.K.T. et al. Phenotypic and genotypic characterizations of bacteria isolated from the respiratory microbiota of healthy turkeys with potential for probiotic composition. Vet Res Commun 48, 381–390 (2024). https://doi.org/10.1007/s11259-023-10217-8
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DOI: https://doi.org/10.1007/s11259-023-10217-8