Abstract
Mycoplasma ovipneumoniae is an important etiological agent of sheep respiratory disease worldwide. Here, we describe the first isolation and draft genome sequence of M. ovipneumoniae strain USP-BR2017 retrieved from tracheobronchial lavage of a sheep showing clinical signs of respiratory disease in the Rio de Janeiro State, Brazil. The culture of tracheobronchial lavage resulted in glucose-fermenting fried egg colonies, which were identified as M. ovipneumoniae by polymerase chain reaction. The genome was sequenced using the Illumina NextSeq 2000 and de novo assembled using SPAdes. The genome of the sequenced organism presented an approximate size of 1,122,253 bp. The annotation revealed 773 coding DNA sequences (CDSs), 806 genes, three rRNAs, and 30 tRNAs. Data analysis revealed M. ovipneumoniae strain USP-BR2017 contains a few virulence genes, including the hemolysing C gene (hlyC). In addition, strain USP-BR2017 showed high identity over the 16S rRNA gene with other sheep isolates from China and United States. This first description of M. ovipneumoniae in diseased Brazilian sheep demonstrates the importance of continuous surveillance and diagnostics of pathogens causing respiratory disease in sheep in Brazil.
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Data availability
Raw reads and other information are publicly available at GenBank (BioProject: PRJNA833450; BioSample: SAMN27991299; Accession: JAMWEX000000000). Codes are publicly available at www.github.com/nataliagaeta29
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Acknowledgements
The authors are thankful to the Coordination of Superior Level Staff Improvement (CAPES) and the National Council for Scientific and Technological Development (CNPq). NCG is a research fellow of FAPESP (#2020/15008-0). Lilian Gregory is a research fellow of CNPq (# 312249/2017-9).
Funding
This work was supported by the USDA National Institute of Food and Agriculture and Hatch Appropriations under Projects #PEN04752, Accession #1023328 awarded to Erika Ganda.
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Natália Gaeta: Conceptualization, Methodology, Formal Analysis, Investigation, Writing – Original Draft. Ana Márcia Guimarães: Methodology, Writing – Review & Editing. Jorge Timenetsky: Writing – Review & Editing. Stephanie Clouser: Investigation. Lilian Gregory: Writing – Review & Editing, Supervision. Erika Ganda: Writing – Review & Editing, Supervision, Funding acquisition.
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Gaeta, N.C., de Sá Guimarães, A.M., Timenetsky, J. et al. The first Mycoplasma ovipneumoniae recovered from a sheep with respiratory disease in Brazil – draft genome and genomic analysis. Vet Res Commun 46, 1311–1318 (2022). https://doi.org/10.1007/s11259-022-09972-x
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DOI: https://doi.org/10.1007/s11259-022-09972-x