Abstract
Secretions of beneficial intestinal bacteria can inhibit the growth and biofilm formation of a wide range of microorganisms. Curcumin has shown broad spectrum antioxidant, anti-inflammatory, and antimicrobial potential. It is important to evaluate the influence of these secretions with bioactive peptides, in combination with curcumin, to limit growth and inhibit biofilm formation of pathogenic bacteria of importance in aquaculture. In the present study, the supernatants of Lactoccocus lactis NZ9000, Lactobacillus rhamnosus GG and Pediococcus pentosaceus NCDO 990, and curcumin (0,1,10,25 and 50 μM) were used to evaluate their efficacy in growth, inhibition biofilm and membrane permeability of Aeromonas hydrophila CAIM 347 (A. hydrophila). The supernatants of probiotics and curcumin 1,10 and 25 μM exerted similar effects in reducing the growth of A. hydrophila at 12 h of interaction. The supernatants of the probiotics and curcumin 25 and 50 μM exerted similar effects in reducing the biofilm of A. hydrophila. There is a significant increase in the membrane permeability of A. hydrophila in interaction with 50 μM curcumin at two hours of incubation and with the supernatants separately in the same period. Different modes of action of curcumin and bacteriocins separately were demonstrated as effective substitutes for antibiotics in containing A. hydrophila and avoiding the application of antibiotics. The techniques implemented in this study provide evidence that there is no synergy between treatments at the selected concentrations and times.
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The data that support the findings of this study are available on request from the corresponding author.
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Statistics analysis and graphs were made in Graph Pad Prism (RRID:SCR_002798) version 6.01.
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This work was supported by Consejo Nacional de Ciencia y Tecnología (CONACyT) for grant 666132 and partially financed by Autonomous University of Aguascalientes (UAA).
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All authors contributed to the study conception and design. Material preparation, data collection and analysis were performed by DIM, JVJ, MMO, AQS, SLMH. The first draft of the manuscript was written by David Ibarra-Martínez and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.
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Ibarra-Martínez, D., Muñoz-Ortega, M.H., Quintanar-Stephano, A. et al. Antibacterial activity of supernatants of Lactoccocus lactis, Lactobacillus rhamnosus, Pediococcus pentosaceus and curcumin against Aeromonas hydrophila. In vitro study. Vet Res Commun 46, 459–470 (2022). https://doi.org/10.1007/s11259-021-09871-7
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DOI: https://doi.org/10.1007/s11259-021-09871-7