Abstract
Capture myopathy (CM) is a metabolic disease associated with mortality in mass boma captured (MBC) wildlife. The condition is induced by the forced pursuit, capturing, and restraint of wild animals, although its causal biology remains to be confirmed. A core feature of MBC-CM is rhabdomyolysis, which is associated with myoglobinuria and hyperthermia. Towards developing a translational model of CM-associated rhabdomyolysis, we investigated forced treadmill running to induce physical exhaustion and trigger rhabdomyolysis in Sprague Dawley (SD) rats. Twenty-four (24) SD rats (12 per sex) were subjected to treadmill habituation in a speed-tiered approach. Forty-eight hours after the last habituation session, one strenuous exercise (SE) session was performed at 75% of the theoretical VO2MAX (30 m/min) until animals reached physical exhaustion. Core and skin surface temperatures were measured before the SE session and after rats reached exhaustion, after which a 1-h—cumulative urine sample was collected, and the myoglobin content assayed. We show that most SE, but not control-exposed (non-exercise) rats presented with myoglobinuria, while core and surface body temperatures in both male and female rats were significantly higher post-exercise. This pre-clinical model framework shows potential for investigating the pathophysiology of MBC-CM.
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This work was funded entirely from a research grant awarded to BHH by Wildlife Pharmaceuticals (Pty) Ltd, White River, South Africa.
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DWW and CL conceptualised, planned and executed the work presented, wrote the draft version of this paper and did the final revision following co-author input. FPV assisted with myoglobin and temperature measurements and reviewed the draft paper. BHH and LM provided intellectual input, and read and revised the draft and final versions of this paper.
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This work was approved by the AnimCare Animal Research Ethics Committee of the North-West University (NHREC reg. no. AREC-130913-015), prior to onset (approval number: NWU-00576-19-A5).
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The authors wish to convey their gratitude to Wildlife Pharmaceuticals (Pty) Ltd who were the kind sponsors of this work.
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Lubbe, C., Harvey, B.H., Viljoen, F.P. et al. Forced running-induced rhabdomyolysis in the Sprague–Dawley rat: towards a rodent model of capture myopathy. Vet Res Commun 45, 459–465 (2021). https://doi.org/10.1007/s11259-021-09840-0
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DOI: https://doi.org/10.1007/s11259-021-09840-0