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Veterinary Research Communications

, Volume 42, Issue 2, pp 137–143 | Cite as

B-mode ultrasound examination of canine mammary gland neoplastic lesions of small size (diameter < 2 cm)

  • Iacopo Vannozzi
  • Matteo Tesi
  • Marta Zangheri
  • Viola Maria Innocenti
  • Alessandra Rota
  • Simonetta Citi
  • Alessandro Poli
Original Article

Abstract

Ultrasonography is a valuable tool for the evaluation of neoplastic lesions in the dog and there is a growing interest in the use of this technique for the stadiation of canine mammary tumours. An accurate assessment of small sized nodules facilitates the stadiation of the mammary lesions and helps the clinician in the choice of the most indicated surgical therapy. The aim of this study was to identify those ultrasound criteria that may be useful in discriminating between benign and malignant lesions of small size (diameter smaller than 2 cm). Sixty-two nodules, < 2 cm in larger diameter, belonging to thirty-five bitches presented between January 2012 and February 2014 were evaluated. Tumours were observed by conventional ultrasound and assessed for: shape (regular-irregular), limit (defined-ill-defined), margins (regular-irregular), echogenicity (hypoechoic-isoechoic-hyperecoic), echotexture (homogeneus-heterogeneus), presence of hyperecoic halo, distal acoustic enhancement or shadowing and surrounding tissue alterations. Among the alterations in surrounding tissues, the disruption of the glandular tissue and the increase in echogenicity of the peritumoral tissues were assessed. Thereafter, bitches were subjected to mastectomy and nodules were evaluated histologically. None of the ultasound criteria considered in the current study showed a statistically significant relation with malignancy, except for the presence of alterations in the tissue surrounding the nodules. According to our results, this characteristic may indicate malignancy, however its subjectivity may affect the applicability in clinical practice. In conclusions, conventional ultrasound in bitches had a limited ability in discriminating benign and malignant mammary gland neoplastic lesions of small size (diameter < 2 cm).

Keywords

Dog Canine Mammary tumours Ultrasound Image analysis Ultrasound criteria 

Notes

Compliance with ethical standards

Conflict of interest

The authors declare that there is no conflict of interests regarding the publication of this paper.

References

  1. Adamietz BR, Meier-Meitinger M, Fasching P, Beckmann M, Hartmann A, Uder M, Häberle L, Schulz-Wendtland R, Schwab SA (2011) New diagnostic criteria in real-time elastography for the assessment of breast lesions. Ultraschall Med 32:67–73.  https://doi.org/10.1055/s-0029-1245821 CrossRefPubMedGoogle Scholar
  2. Antuofermo E, Miller M, Pirino S, Xie J, Badve S, Mohammed SI (2007) Spontaneous mammary intraepithelial lesions in dogs—a model of breast cancer. Cancer Epidemiol Biomark Prev 16:2247–2256.  https://doi.org/10.1158/1055-9965.EPI-06-0932 CrossRefGoogle Scholar
  3. Bastan A, Özenc E, Pir Yagci I, Baki Acar D (2009) Ultrasonographic evaluation of mammary tumors in bitches. Kafkas Üniversitesi Veteriner Fakültesi Dergisi 1:81–86.  https://doi.org/10.9775/kvfd.2008.79-A Google Scholar
  4. Bulnes AG, Fernandez PG, Aguirre AMM, Sanchez De La Muela M (1998) Ultrasonographic imaging of canine mammary tumors. Vet Rec 143:687–689.  https://doi.org/10.1136/vr.143.25.687 Google Scholar
  5. Calas MJG, Koch HA, Dutra MVP (2007) Breast ultrasound: evaluation of echographic criteria for differentiation of breast lesions. Radiol Bras 40:1–7.  https://doi.org/10.1590/S0100-39842007000100003 CrossRefGoogle Scholar
  6. Cassali GD, Lavalle GE, Ferreira E, Estrela-Lima A, De Nardi AB, Ghever C et al (2014) Consensus for the diagnosis, prognosis and treatment of canine mammary tumors – 2013. Braz J Vet Pathol 7(2):38–69Google Scholar
  7. Dorn CR, Taylor DON, Schneider R, Hibbard HH, Klauber MR (1968) Survey of animal neoplasms in Alameda and Contra Costa Counties, California. II. Cancer morbidity in dogs and cats from Alameda County. J Natl Cancer Inst 40:307–318.  https://doi.org/10.1093/jnci/40.2.307 PubMedGoogle Scholar
  8. Feliciano MAR, Vicente WR, Silva MA (2012) Conventional and Doppler ultrasound for the differentiation of benign and malignant canine mammary tumours. J Small Anim Pract 53:332–337.  https://doi.org/10.1111/j.1748-5827.2012.01227.x CrossRefPubMedGoogle Scholar
  9. Feliciano MAR, Maronezi MC, Pavan L, Castanheira TL, Simões APR, Carvalho CF, Canola JC, Vicente WRR (2014) ARFI elastography as a complementary diagnostic method for mammary neoplasia in female dogs–preliminary results. J Small Anim Pract 55 (10):504–508.  https://doi.org/10.1111/jsap.12256 CrossRefPubMedGoogle Scholar
  10. Feliciano MAR, Uscategui RAR, Maronezi MC, Simões APR, Silva P, Gasser B, Pavan L, Carvalho CF, Canola JC, Vicente WRR (2017) Ultrasonography methods for predicting malignancy in canine mammary tumors. PLoS One 12(5):e0178143.  https://doi.org/10.1371/journal.pone.0178143 CrossRefPubMedPubMedCentralGoogle Scholar
  11. Itoh A, Ueno E, Tohno E, Kamma H, Takahashi H, Shiina T, Yamakawa M, Matsumura T et al (2006) Breast disease: clinical application of US elastography for diagnosis. Radiology 239:341–350.  https://doi.org/10.1148/radiol.2391041676 CrossRefPubMedGoogle Scholar
  12. Kurzman ID, Gilbertson SR (1986) Prognostic factors in canine mammary tumors. Semin Vet Med Surg 1:25–32Google Scholar
  13. Millanta F, Calandrella M, Bari G, Niccolini M, Vannozzi I, Poli A (2005) Comparison of steroid receptor expression in normal, dysplastic, and neoplastic canine and feline mammary tissues. Res Vet Sci 79(3):225–232.  https://doi.org/10.1016/j.rvsc.2005.02.002 CrossRefPubMedGoogle Scholar
  14. Misdorp W, Else RW, Hellman E, Lipscomb TP (1999) Histological classification of mammary tumors of the dog and the cat. The Armed Forces Institute of Pathology in cooperation with the American Registry of Pathology and the World Health Organization Collaboration Center for Worldwide Reference on Comparative Oncology, Washington, DC pp 1–49Google Scholar
  15. Mohammed SI, Meloni GB, Pinna Parpaglia ML, Marras V, Burrai GP, Meloni F, Pirino S, Antuofermo E (2011) Mammography and ultrasound imaging of preinvasive and invasive canine spontaneous mammary cancer and their similarities to human breast cancer. Cancer Prev Res 4(11):1790–1798.  https://doi.org/10.1158/1940-6207.CAPR-11-0084 CrossRefGoogle Scholar
  16. Moulton JE (1999) Tumours in domestic animals. In: University of California Press, 3rd edn. Berkley, California, pp 518–543Google Scholar
  17. North S, Banks T (2009) Introduction to small animal oncology. Saunders Elsevier, LondonGoogle Scholar
  18. Nyman HT, Kristensen AT, Lee MH, Martinussen T, Mcevoy FJ (2006a) Characterization of canine superficial tumors using gray-scale b mode, color flow mapping, and spectral doppler ultrasonography - a multivariate study. Vet Radiol Ultrasound 47:192–198.  https://doi.org/10.1111/j.1740-8261.2006.00127.x CrossRefPubMedGoogle Scholar
  19. Nyman HT, Nielsen OL, Mcevoy FJ, Lee MH, Martinussen T, Hellmén E, Kristensen AT (2006b) Comparison of B-mode and Doppler ultrasonographic findings with histologic features of benign and malignant mammary tumors in dogs. Am J Vet Res 67:985–991.  https://doi.org/10.2460/ajvr.67.6.985 CrossRefPubMedGoogle Scholar
  20. Paulinelli RR, Freitas-Junior R, de Lucena CE, Moreira MA, de Moraes VA, Bernardes-Junior JR, da Silva Rocha Vidal C, Ruiz AN, Lucato MT, da Costa NG, Teixeira DA (2011) Sonobreast: predicting individualized probabilities of malignancy in solid breast masses with echographic expression. Breast J 17(2):152–159.  https://doi.org/10.1111/j.1524-4741.2010.01046.x CrossRefPubMedGoogle Scholar
  21. Philibert JC, Snyder PW, Glickman N, Glickman L, Knapp D, Waters D (2003) Influence of host factors on survival in dogs with malignant mammary gland tumors. J Vet Intern Med 17:102–106.  https://doi.org/10.1111/j.1939-1676.2003.tb01330.x CrossRefPubMedGoogle Scholar
  22. Queiroga FL, Perez-Alenza MD, Silvan G, Pena L, Lopes C, Illera JC (2005) Role of steroid hormones and prolactin in canine mammary cancer. J Steroid Biochem Mol Biol 94:181–187.  https://doi.org/10.1016/j.jsbmb.2004.12.014 CrossRefPubMedGoogle Scholar
  23. Queiroga FL, Raposo T, Carvalho MI, Prada J, Pires I (2011) Canine mammary tumours as a model to study human breast cancer: most recent findings. In Vivo 25(3):455–465PubMedGoogle Scholar
  24. Quieroga FL, Lopes C (2002) Canine mammary tumors – new perspectives. Annals of Congress of Veterinary Science, Oeiras, Brazil, pp 183–190Google Scholar
  25. Rahbar G, Sie AC, Hansen GC, Prince JS, Melany ML, Reynolds HE, Jackson VP, Sayre JW, Bassett LW (1999) Benign versus malignant solid breast masses: US differentiation. Radiology 213:889–894.  https://doi.org/10.1148/radiology.213.3.r99dc20889 CrossRefPubMedGoogle Scholar
  26. Skaane P, Engedal K (1998) Analysis of sonographic features in the differentiation of fibroadenoma and invasive ductal carcinoma. Am J Roentgenol 170:109–114.  https://doi.org/10.2214/ajr.170.1.9423610 CrossRefGoogle Scholar
  27. Sleeckx N, de Rooster H, Veldhuis Kroeze EJB, Van Ginneken C, Van Brantegem L (2011) Canine mammary tumours, an overview. Reprod Domest Anim 46(6):1112–1131.  https://doi.org/10.1111/j.1439-0531.2011.01816.x CrossRefPubMedGoogle Scholar
  28. Soler M, Dominguez E, Lucas X, Novellas R, Gomes-Coelho KV, Espada Y, Agut A (2016) Comparison between ultrasonographic findings of benign and malignant canine mammary gland tumours using B-mode, colour Doppler, power Doppler and spectral Doppler. Res Vet Sci 107:141–146.  https://doi.org/10.1016/j.rvsc.2016.05.015 CrossRefPubMedGoogle Scholar
  29. Sorenmo KU, Shofer FS, Goldschmidt MH (2000) Effect of spaying and timing of spaying on survival of dogs with mammary carcinoma. J Vet Intern Med 14:266–270.  https://doi.org/10.1177/0300985810389480 CrossRefPubMedGoogle Scholar
  30. Tagawa M, Kanai E, Shimbo G, Kano M, Kayanuma H (2016) Ultrasonographic evaluation of depth–width ratio (D/W) of benign and malignant mammary tumors in dogs. J Vet Med Sci 78(3):521–524.  https://doi.org/10.1292/jvms.15-0456 CrossRefPubMedGoogle Scholar
  31. Vail DM, MacEwen EG (2000) Spontaneously occurring tumors of companion animals as models for human cancer. Cancer Investig 18:781–792.  https://doi.org/10.3109/07357900009012210 CrossRefGoogle Scholar
  32. Watermann DO, Tempfer C, Hefler LA, Parat C, Stickeler E (2005) Ultrasound morphology of invasive lobular breast cancer is different compared with other types of breast cancer. Ultrasound Med Biol 31(2):167–174.  https://doi.org/10.1016/j.ultrasmedbio.2004.11.005 CrossRefPubMedGoogle Scholar
  33. Yi A, Cho N, Chang JM, Koo HR, La Yun B, Moon WK (2012) Sonoelastography for 1,786 non-palpable breast masses: diagnostic value in the decision to biopsy. Eur Radiol 22:1033–1040.  https://doi.org/10.1007/s00330-011-2341-x CrossRefPubMedGoogle Scholar
  34. Zhou J, Zhan W, Dong Y, Yang Z, Zhou C (2014) Stiffness of the surrounding tissue of breast lesions evaluated by ultrasound elastography. Eur Radiol 24(7):1659–1667.  https://doi.org/10.1007/s00330-014-3152-7 CrossRefPubMedGoogle Scholar
  35. Zonderland HM, Coerkamp EG, Hermans J, van de Vijver MJ, van Voorthuisen AE (1999) Diagnosis of breast cancer: contribution of US as an adjunct to mammography. Radiology 213:413–422.  https://doi.org/10.1148/radiology.213.2.r99nv05413 CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media B.V., part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Veterinary SciencesUniversity of PisaPisaItaly
  2. 2.Veterinary Center “Campo di Marte”LivornoItaly

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