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Veterinary Research Communications

, Volume 42, Issue 2, pp 131–135 | Cite as

Detection of Trypanosoma vivax DNA in semen from experimentally infected goats

  • Nicholas Morais Bezerra
  • Gabriela Hémylin Ferreira Moura
  • Hélio Noberto de AraújoJr.
  • Francisco Silvestre Brilhante Bezerra
  • Kaliane Alessandra Rodrigues de Paiva
  • Kizzy Millenn de Freitas Mendonça Costa
  • Wirton Peixoto Costa
  • Dayse Ariane Soares Medeiros
  • Jael Soares Batista
Original Article

Abstract

The present work aimed to investigate the presence of T. vivax DNA in the semen of experimentally infected goats. Twelve male goats native to the Brazilian Northeast, adults, were randomly assigned to two experimental groups: the infected group consisting of six goats infected intravenously with 0.5 mL of blood containing approximately 1.25 × 105 trypomastigotes of T. vivax, and a control group composed of six uninfected goats. After the infection, clinical examinations aiming to evaluate rectal temperature, parasitemia and hematocrit were performed. Semen samples were collected from goats by electroejaculation on the 7th, 14th and 21st days post-infection (dpi). The recombinant DNA-encoding gene encoding the L-like-specific gene for T. vivax. The infection was characterized by increased rectal temperature, high parasitemia and significant reduction of hematocrit values. Results for T. vivax DNA detection using TviCatL-PCR were positive in all semen samples from the infected group collected on 7th, 14th and 21st dpi. The presence of T. vivax DNA in 7th dpi suggests the early invasion of the parasite in the reproductive organs. Also, the finding of T. vivax DNA in all periods analyzed may suggest the continued elimination of the parasite in the semen, which may increase the chances of sexual transmission. Thus, T. vivax DNA is recorded for the first time in the semen of infected goats. Thus, these data are of great importance, since the detection of the T. vivax genetic material in the semen may point to the possibility that the parasite may be transmitted through the sexual pathway.

Keywords

PCR Semen Small ruminant Tripanossomiase 

Notes

Compliance with ethical standards

Ethical approval:

All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.

Conflict of interest

The authors declare that they have no conflict of interest.

References

  1. Adamu S, Fatihu MY, Useh NM, Mamman M, Sekoni VO, Esievo KAN (2007) Sequential testicular and epididymal damage in zebu bulls experimentally infected with Trypanosoma vivax. Vet Parasitol 143:29–34.  https://doi.org/10.1016/j.vetpar.2006.07.022 CrossRefPubMedGoogle Scholar
  2. Almeida KS, Freitas FLC, Tebaldi JH, Alessi AC, Machado RZ, Nascimento AA (2010) Alterações clínicas, histopatológicas e enzimáticas em ovinos infectados experimentalmente por Trypanosoma vivax. Ciênc Anim Bras 11:669–676.  https://doi.org/10.5216/cab.v11i3.5971 CrossRefGoogle Scholar
  3. Batista JS, Bezerra FSB, Lira RA, Carvalho JRG, Rosado Neto AM, Petri AA, Teixeira MMG (2008) Clinical, epidemiological and pathological signs of natural infection in cattle by Trypanosoma vivax in Paraíba, Brazil. Pesq Vet Bras 28:63–69.  https://doi.org/10.1590/S0100-736X2008000100010 CrossRefGoogle Scholar
  4. Batista JS, Rodrigues CM, García HA, Bezerra FS, Olinda RG, Teixeira MM, Soto-Blanco B (2011) Association of Trypanosoma vivax in extracellular sites with central nervous system lesions and changes in cerebrospinal fluid in experimentally infected goats. Vet Res 42:63–70.  https://doi.org/10.1186/1297-9716-42-63 CrossRefPubMedPubMedCentralGoogle Scholar
  5. Batista JS, Rodrigues CMF, Olinda RG, Silva TMF, Vale RG, Câmara ACL, Rebouças RES, Bezerra FSB, García HA, Teixeira MMG (2012) Highly debilitating natural Trypanosoma vivax infections in brazilian calves: epidemiology, pathology, and probable transplacental transmission. Parasitol Res 110:73–80.  https://doi.org/10.1007/s00436-011-2452-y CrossRefPubMedGoogle Scholar
  6. Bezerra FSB, Garcia HA, Alves HM, Oliveira IRS, Silva AE, Teixeira MMG, Batista JS (2008) Trypanosoma vivax in the testicular and epididymal tissues of experimentally infected sheep. Pesq Vet Bras 28:575–582.  https://doi.org/10.1590/S0100-736X2008001200002 CrossRefGoogle Scholar
  7. Bittar JFF, Bassi PB, Moura DM, Garcia GC, Martins-Filho OA, Vasconcelos AB, Costa-Silva MF, Barbosa CP, Araújo MSS, Bittar ER (2015) Evaluation of parameters related to libido and semen quality in zebu bulls naturally infected with Trypanosoma vivax. BMC Vet Res 11:261–268.  https://doi.org/10.1186/s12917-015-0571-x CrossRefPubMedPubMedCentralGoogle Scholar
  8. Brener Z (1961) Contribution to the study of experimental therapy of Chagas’ disease.Doctoral thesis.<https://science.report/pub/36797551> (Accessed 10 Januany 2017).
  9. Cortez AP, Rodrigues AC, Garcia HA, Neves L, Batista JS, Bengaly Z, Paiva F, Teixeira MMG (2009) Cathepsin L-like Genes of Trypanosoma vivax from Africa and south America - characterisation, relationships and diagnostic implications. Mol Cell Probes 23:44–51.  https://doi.org/10.1016/j.mcp.2008.11.003 CrossRefPubMedGoogle Scholar
  10. Dias JCP, Amato Neto V, Luna EJA (2011) Alternative mechanisms of transmission of Trypanosoma cruzi in Brazil and suggestions for its prevention. Rev Soc Bras Med Trop 44:1–5.  https://doi.org/10.1590/S0037-86822011005000032 CrossRefGoogle Scholar
  11. Gutierrez C, Corbera JA, Morales M, Büscher P (2006) Trypanosomosis in goats: current status. Ann N Y Acad Sci 1081:300–310.  https://doi.org/10.1196/annals.1373.040 CrossRefPubMedGoogle Scholar
  12. Jones T, Dávila AMR (2001) Trypanosama vivax: out of Africa. Trends Parasitol 2:99–101.  https://doi.org/10.1016/S1471-4922(00)01777-3 CrossRefGoogle Scholar
  13. Leger M, Vienne M (1919) Epizoootie à trypanosomes ches les boidés de la Guyiane Francaise. Bull Soc Pathol Exot 12:258–266.Google Scholar
  14. Sekoni VO (1994) Reproductive disorders caused by animal trypanosomiases: a review. Theriogenol 42:557–570. https://www.ncbi.nlm.nih.gov/pubmed/16727562. Accessed 13 December 2016
  15. Sekoni VO, Rekwot PI, Bawa EK (2004) Effects of Trypanosoma vivax and Trypanosoma congolense infections on the reaction time and semen characteristics of Zebu (Bunaji) x Friesian Crossbred Bulls. Theriogenol 61:55–62.  https://doi.org/10.1016/S0093-691X(03)00183-3 CrossRefGoogle Scholar
  16. Silva RAMS., Seidl A, Ramirez L, Dávila AMR (2002) Trypanosoma evansi e Trypanosoma vivax: Biology, diagnostic and control, 21th edn. Embrapa Pantanal, BrasilGoogle Scholar
  17. Silva TMF, Olinda RG, Rodrigues CMF, Câmara ACL, Lopes FC, Coelho WAC, Ribeiro MFB, Freitas CIA, Teixeira MMG, Batista JS (2013) Pathogenesis of reproductive failure induced by Trypanosoma vivax in experimentally infected pregnant ewes. Vet Res 44:1–9.  https://doi.org/10.1186/1297-9716-44-1 CrossRefPubMedPubMedCentralGoogle Scholar
  18. Silva AS, Campigotto G, Sczesny TR, Pazinato R, Albani KD, Klauck V, Grosskopf RK, Topazio JP, Galli GM, Cazzarotto CJ, Ferreira R, Tonin AA, Monteiro SG, Weiss PHE, Miletti LC, Leal MLR (2016) Experimental infection with Trypanosoma evansi in rams: presence of parasite in semen, investigation of sexual transmission, hematological, and biochemical alterations. Comp Clin Pathol 25:841–846.  https://doi.org/10.1007/s00580-016-2271-8 CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V., part of Springer Nature 2018

Authors and Affiliations

  • Nicholas Morais Bezerra
    • 1
  • Gabriela Hémylin Ferreira Moura
    • 1
  • Hélio Noberto de AraújoJr.
    • 1
  • Francisco Silvestre Brilhante Bezerra
    • 1
  • Kaliane Alessandra Rodrigues de Paiva
    • 1
  • Kizzy Millenn de Freitas Mendonça Costa
    • 1
  • Wirton Peixoto Costa
    • 1
  • Dayse Ariane Soares Medeiros
    • 1
  • Jael Soares Batista
    • 1
  1. 1.Center for Agrarian SciencesFederal Rural of the Semi-Arid UniversityMossoroBrazil

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