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Investigation of intra-herd spread of Mycobacterium caprae in cattle by generation and use of a whole-genome sequence

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Abstract

Single nucleotide polymorphisms (SNPs) calculated from whole genome sequencing (WGS) are ideally suited to study evolutionary relationships of pathogens and their epidemiology. Mycobacterium caprae infections have been documented frequently in cattle and red deer along the Bavarian and Austrian Alps during the last decade. However, little is still known about the transmission within cattle holdings and possible alterations of the genomes of M. caprae during such events. The aim of this study was to study the molecular epidemiology of bovine tuberculosis (bTB) in selected herds based on isolate-specific genome-wide SNPs and to perform a phylogenetic network analysis. In total, 61 M. caprae isolates were collected originating from eight cattle farms over a period of twelve years between 2004 and 2015. Analysis of their sequence data revealed that the M. caprae isolates of an affected farm differ at all in a few SNPs. In contrast, many more SNPs were found when comparing the M. caprae genomes originating from different herds. The results demonstrated that the spread of bTB in the affected farms occurred by direct transmission between the members of each herd rather than between herds and a M. caprae introduction in farms after contact events e. g. on summer pastures can readily be traced by WGS analysis. Furthermore, we assembled a nearly complete whole genome sequence of M. caprae derived from several cattle isolates originating from bTB cases in the Bavarian Alpine region.

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Acknowledgement

The authors would like to thank all colleagues from the pathology of the Bavarian Health and Food Safety Authority for macroscopically pre-screening of cattle carcasses and organ samples. We are grateful for excellent support by H. Lang and technicians in bacteriology. Special thanks to A. Graf for his support with the Galaxy platform and J. Boenchendorf for her help extracting the data from the HI-Tier system. We are grateful to J. Mages and K. Bomans from the district veterinary office of Upper Allgäu and F. Götz from East Allgäu for their information. Thanks to N. Ackermann for maintenance of the LGL mycobacteria culture collection. This work was funded by the Bavarian State Ministry of Nutrition, Agriculture and Forestry.

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  1. Bavarian Health and Food Safety Authority, Veterinaerstr. 2, 85764, Oberschleissheim, Germany

    S. Broeckl & M. Buettner

  2. Laboratory for Functional Genome Analysis (LAFUGA), Gene Center, Ludwig-Maximilians-University (LMU) Munich, Feodor-Lynen-Str. 25, 81377, Munich, Germany

    S. Krebs, A. Varadharajan & H. Blum

  3. Bacteriology and Mycology, Institute for Infectious Diseases and Zoonoses, Department of Veterinary Sciences, Faculty of Veterinary Medicine, Ludwig-Maximilians-University (LMU) Munich, Veterinaerstr. 13, 80539, Munich, Germany

    R. K. Straubinger

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Broeckl, S., Krebs, S., Varadharajan, A. et al. Investigation of intra-herd spread of Mycobacterium caprae in cattle by generation and use of a whole-genome sequence. Vet Res Commun 41, 113–128 (2017). https://doi.org/10.1007/s11259-017-9679-8

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