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Pathological aspects of bovine focal fibrogranulomatous proliferative panniculitis (Lechiguana)

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Abstract

Lechiguana is a disease of cattle caused by an interaction between Dermatobia hominis warble and the bacteria Manheimia granulomatis. It is characterized by subcutaneous swellings that grow rapidly and result in death after 3 to 8 months. The objective of this paper was to investigate some vascular and fibrogenic changes of the disease at different lesion stages by histochemical and immunohistochemical techniques. A peculiar histopathological aspect observed during a proliferative phase (before treatment) was the intense vasculitis, described as degenerative and fibro-proliferative, expressed by the oncogene p53, possibly caused by the presence of bacteria in close contact with enthotelial cells, along with dense accumulations of lymphoid cells around venules. The synthesis of collagen fibers during the development of Lechiguana lesions assume a structural aspect of star arrangement with fiber radiation centers that gradually interconnect to design the Extracellular Matrix (ECM) framework, seen by Confocal Laser Scanning Microscopy (CSLM). Angiogenesis was the most characteristic finding in both proliferative and regressive stages as seen by the immunohistochemical expression of cytoskeleton proteins and von Willebrand (Factor VIII-Related Antigen). Additionally, in all tissues samples, active ECM elements like Metalloproteinases (MMPs), Tissue Inhibitors Metalloproteinases (TIMP) and Fibronectin (FN) were mainly associated to vessels structures. The extraordinary regression of exuberant granulation tissue after treatment is undoubtedly associated to the maintenance of the vascular components observed during the regressive phase.

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Conflict of interest

The authors declare that they have no conflict of interest.

Ethical standards

The experiments comply with the current laws of the country in which they were performed.

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Authors and Affiliations

  1. Dom Bosco Catholic University, Campo Grande, MS, Brazil

    G. B. Andrade, H. M. Herrera & W. T. G. Barreto

  2. Regional Diagnostic Laboratory, UFPeL, Pelotas, RS, Brazil

    S. L. Ladeira

  3. Department of Pathology, Oswaldo Cruz Institute, Rio de Janeiro, Brazil

    E. M. Mota, L. G. Caputo & H. L. Lenzi

Authors
  1. G. B. Andrade
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  2. H. M. Herrera
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  3. W. T. G. Barreto
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  4. S. L. Ladeira
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  5. E. M. Mota
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  6. L. G. Caputo
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  7. H. L. Lenzi
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Corresponding author

Correspondence to G. B. Andrade.

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HL Lenzi in memorian.

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Andrade, G.B., Herrera, H.M., Barreto, W.T.G. et al. Pathological aspects of bovine focal fibrogranulomatous proliferative panniculitis (Lechiguana). Vet Res Commun 39, 39–44 (2015). https://doi.org/10.1007/s11259-015-9627-4

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  • DOI: https://doi.org/10.1007/s11259-015-9627-4

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