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Effects of manganese deficiency on the microstructure of proximal tibia and OPG/RANKL gene expression in chicks

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Abstract

Manganese (Mn) deficiency can result in perosis in chicks, but the mechanism of Mn deficiency on tibia development remains poorly understood. Ninety one-day-old Arbor Acres male broiler chickens administered with control diet (60 mg Mn/kg) and Mn-deficient diets (40 mg Mn/kg, 8.7 mg Mn/kg) to investigate the effects of Mn deficiency on morphology of tibia and related signal transduction pathways in broiler chickens. At the age of 42 days, the bone trabecula, damaged osteoblasts and OPG/RANKL mRNA expression levels were investigated by histological assessment, electron microscopic examination and real-time quantitative PCR analysis, respectively. Results of histological observations showed that decreased trabecular thickness, trabecular number and trabecular bone area (%) together with increased trabecular bone separation were involved in perosis induced by Mn deficiency. The most striking ultrastructural modifications involved disruption of nuclear membrane and mitochondria outer membrane, loss of mitochondrion cristae and alteration in endoplasmic reticulum in osteoblasts of the Mn-deficient groups. Likewise, Mn deficiency results in a significant (P < 0.05) decrease in the relative mRNA expression levels of OPG and RANKL with a significantly higher RANKL/OPG ratio (P < 0.05). In conclusion, Mn deficiency can affect the development of tibia in broiler chickens, leading to metaphyseal osteoporosis which may be due to decreased OPG/RANKL mRNA expression.

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Acknowledgments

This work was supported by the foundation for the author of national excellent doctoral dissertation of PR China (No. 201266) and the fund of Fok Ying Tung education foundation under grant No. 141022.

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The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Daizong Road No. 61, Taian, 271018, People’s Republic of China

    Ran Liu, Zhenyong Wang, Zhaojun Wang, Jian Wang & Lin Wang

  2. Feixian Animal Husbandry Bureau, Shandong Linyi, Shengli Road No.93, Feixian, 272400, People’s Republic of China

    Cong Jin

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  1. Ran Liu
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  2. Cong Jin
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  3. Zhenyong Wang
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  4. Zhaojun Wang
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  5. Jian Wang
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  6. Lin Wang
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Correspondence to Zhenyong Wang or Lin Wang.

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Liu, R., Jin, C., Wang, Z. et al. Effects of manganese deficiency on the microstructure of proximal tibia and OPG/RANKL gene expression in chicks. Vet Res Commun 39, 31–37 (2015). https://doi.org/10.1007/s11259-015-9626-5

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  • DOI: https://doi.org/10.1007/s11259-015-9626-5

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