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Molecular cloning and expression analysis of pig CD7

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Abstract

CD7 is an integral membrane protein which mediates an important signal to mediate the differentiation, activation, and regulation of some T cells and NK cells. However, only human and mouse CD7 have been identified and studied among mammalian species. In this study, we cloned pig CD7 cDNA and determined its complete cDNA sequence. Pig CD7 cDNA contained an open reading frame (627 bp) encoding 208 amino acids with well conserved motifs involved in signal transduction within cytoplasmic tail among mammalian species. Pig CD7 mRNA was detected by RT-PCR in mainly lymphoid tissues, indicating the conserved functions of CD7 in pigs. Moreover, we generated soluble pig CD7 fusion immunoglobulin (pig CD7Ig) containing extracellular domain of pig CD7 to test whether pig CD7 binds to pig galectin-3. Flow cytometry and immunohistochemistry analyses indicated that soluble pig CD7Ig can bind to galectin-3 expressed in macrophages and epithelial cells of small intestine. These results help to analyze the structural relationship between CD7 and its ligand transferring signal transduction among mammalian species.

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Fig. 1
Fig. 2

Abbreviations

CHO:

Chinese hamster ovary

GAPDH:

Glyceraldehyde-3-phosphate dehydrogenase

Ig:

Immunoglobulin

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Acknowledgments

This work was supported by a grant from the Next-Generation BioGreen 21 program (No. PJ008089), Rural Development Administration, Republic of Korea and a grant from the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health &Welfare, Republic of Korea (grant number : HI13C0954).

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The authors have no conflict of interest.

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Authors and Affiliations

  1. Division of Biotechnology, School of Life Sciences and Biotechnology, Korea University, Seoul, 136-701, Republic of Korea

    Ju Yeon Lee, Joonbeom Bae & Taehoon Chun

  2. School of Biotechnology, Yeungnam University, Gyeongsan City, 712-749, Republic of Korea

    Inho Choi

  3. Department of Microbiology and Immunology, Seoul National University College of Medicine, Seoul, 110-799, Republic of Korea

    Chung-Gyu Park

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  1. Ju Yeon Lee
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  2. Joonbeom Bae
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  3. Inho Choi
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  5. Taehoon Chun
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Correspondence to Taehoon Chun.

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Lee, J.Y., Bae, J., Choi, I. et al. Molecular cloning and expression analysis of pig CD7. Vet Res Commun 38, 257–263 (2014). https://doi.org/10.1007/s11259-014-9603-4

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