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Profiling of virulence associated genes of Pasteurella multocida isolated from cattle

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Abstract

Pasteurella multocida is a causative agent of many major diseases of which haemorrhagic septiciemia (HS) in cattle & a buffalo is responsible for significant losses to livestock sector in India and south Asia. The disease outcome is affected by various host- and pathogen-specific determinants. Several bacterial species-specific putative virulence factors including the capsular and virulence associated genes have been proposed to play a key role in this interaction. A total of 23 isolates of P. multocida were obtained from 335 cases of various clinically healthy and diseased cattle. These isolates were examined for capsule synthesis genes (capA, B, D, E and F) and eleven virulence associated genes (tbpA, pfhA, toxA, hgbB, hgbA, nanH, nanB, sodA, sodC, oma87 and ptfA) by PCR. A total of 19 P. multocida isolates belonging to capsular type B and 4 of capsular type A were isolated. All isolates of capsular type B harboured the virulence associated genes: tbpA, pfhA, hgbA, sodC and nanH, coding for transferrin binding protein, filamentous hemagglutinin, haemoglobin binding protein, superoxide dismutase and neuraminidases, respectively; while isolates belonging to capsular type A also carried tbpA, pfhA, hgbA and nanH genes. Only 50 % of capsular type A isolates contained sodC gene while 100 % of capsular type B isolates had sodC gene. The gene nanB and toxA were absent in all the 23 isolates. In capsular type A isolates, either sodA or sodC gene was present & these genes did not occur concurrently. The presence of virulence associated gene ptfA revealed a positive association with the disease outcome in cattle and could therefore be an important epidemiological marker gene for characterizing P. multocida isolates.

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Acknowledgements

Authors wish to thank Indian Council of Agricultural Research for providing grant to carry out this work; H.P. Animal Husbandry Department & HPKV, Palampur for logistic support and Dr. Varun Sankhyan for his help with statistical analysis.

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Authors and Affiliations

  1. Department of Veterinary Microbiology, Dr. G.C. Negi College of Veterinary & Animal Sciences, CSK Himachal Pradesh Agricultural University, Palampur, Himachal Pradesh, 176062, India

    Subhash Verma, Mandeep Sharma, Shailja Katoch, Lovit Verma, Sandeep Kumar, Vishal Dogra, Rajesh Chahota & Prasenjit Dhar

  2. Department of Veterinary Biochemistry, CSK Himachal Pradesh Agricultural University, Palampur, Himachal Pradesh, 176062, India

    Geetanjali Singh

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  1. Subhash Verma
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  2. Mandeep Sharma
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  3. Shailja Katoch
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  4. Lovit Verma
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Correspondence to Subhash Verma.

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Verma, S., Sharma, M., Katoch, S. et al. Profiling of virulence associated genes of Pasteurella multocida isolated from cattle. Vet Res Commun 37, 83–89 (2013). https://doi.org/10.1007/s11259-012-9539-5

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