Veterinary Research Communications

, Volume 36, Issue 1, pp 47–55 | Cite as

Experimental caprine coccidiosis caused by Eimeria arloingi: Morphopathologic and electron microscopic studies

  • Mohammad Hashemnia
  • Azizollah Khodakaram-Tafti
  • Seyed Mostafa Razavi
  • Saeed Nazifi
Original Article

Abstract

The progressive morphohistopathologic changes, distribution pattern of lesions and ultrastructural characteristics in Eimeria arloingi infection were investigated in experimentally infected kids. The 18 newborn animals allocated to 3 equal groups. Two of groups, A, B were inoculated with a single dose of 1 × 103 and1 × 105 sporulated oocysts of E. arloingi, respectively. At 7, 14, 21, 28, 35, and 42 days postinoculation (DPI), 1 kid from each group was necropsied for pathologic and ultrastructural studies. Progressive lesions were present at 21, 28, 35 and 42 DPI in the jejunum, ileum, cecum with fewer in the duodenum and proximal colon. The oocysts shedding begin between 16 to 18 DPI. Grossly, minimal changes were observed at 21 DPI as few whitish plaques or nodules and advanced lesions at 42 DPI as pseudoadenomatous pattern in the mucosa and a cerebriform pattern on the serosal surface of jejunum and ileum. Early histopathologic lesions due to schizogony phase were including presence of intracytoplasmic developmental stages of the parasite such as trophozoites, immature to mature schizonts and mild infiltration of inflammatory cells. In late lesions due to various stages of gametogony, the histological pattern was mainly remarkable hyperplasia of the villi and crypts epithelial cells, eventually developed into papillary projections of reactive epithelium. The mesenteric lymph nodes showed a few numbers of large schizonts in the cortical lacteals. This study showed E. arloingi as a highly pathogenic species for kids, the incubation period was 16–18 days and the main target organ was jejunum with characteristic morphohistopathologic lesions.

Keywords

Experimental infection Eimeria arloingi Morphopathologic lesions Coccidiosis Kids 

References

  1. Abdel-Ghaffar FA (1990) Electron microscope studies on Eimeria arvicanthi: macrogametogenesis and oocyst formation. Proc Zool Soc AR Egypt 18:334–343Google Scholar
  2. Abo-Shehada MN, Abo-Farieha HA (2003) Prevalence of Eimeria species among goats in northern Jordan. Small Rum Res 49:109–113CrossRefGoogle Scholar
  3. Al-Amery MAY, Hasso SA (2002) Laboratory diagnosis of novel species of Theileria hirci, Eimeria caprovina and Eimeria pallida in goats in Iraq. Small Rum Res 44:163–166CrossRefGoogle Scholar
  4. Al-Hoot AA (1997) Ultrastructural study of micro- and macro-gametogenesis of Eimeria mehlhorni infecting the field rat Arvicanthis niloticus niloticus in Egypt. J Egypt Ger Soc Zool 23(D):93–119Google Scholar
  5. An J, Wang M, Kong F, Yin P (2001) Ultrastructral studies on the macrogametogenesis and oocyst forming of Eimeria tenella. Chinese J Vet Sci 21:559–561Google Scholar
  6. Balicka-Ramisz A (1999) Studies on coccidiosis in goats in Poland. Vet Parasitol 81:347–349PubMedCrossRefGoogle Scholar
  7. Borgsteede FHM, Dercksen D (1996) Coccidial and helminth infections in goats kept indoors in the Netherlands. Vet Parasitol 61:321–326PubMedCrossRefGoogle Scholar
  8. Chhabra RC, Pandey VS (1991) Coccidia of goats in Zimbabwe. Vet Parasitol 39:199–205PubMedCrossRefGoogle Scholar
  9. Cox FEG (1998) Control of coccidiosis: lessons from other sporozoa. Int J Parasitol 28(1):165–179PubMedCrossRefGoogle Scholar
  10. Dai YB, Lin MC, Zhang SX, Fu AQ (1991) Hepatic coccidiosis in the goat. Int J Parasitol 21:381–382PubMedCrossRefGoogle Scholar
  11. Dai YB, Liu XY, Liu M, Tao JP (2006) Pathogenic effects of the coccidium Eimeria ninakohlyakimovae in goats. Vet Res Commun 30:149–160PubMedCrossRefGoogle Scholar
  12. Dougschies A, Najdrowski M (2005) Eimeriosis in cattle: current understanding. J Vet Med 52:417–427CrossRefGoogle Scholar
  13. Entzeroth R, Scholtyseck E, Sezen IY (1981) Fine structural study of Eimeria truncata from domestic goose (Anser anser dom.). Z Parasitenkd 66:1–7PubMedCrossRefGoogle Scholar
  14. Faizal ACM, Rajapakse RPVJ (2001) Prevalence of coccidia and gastrointestinal nematode infections in crossbred goats in the dry areas of Sri Lanka. Small Rum Res 40:233–238CrossRefGoogle Scholar
  15. Foreyt WJ (1990) Coccidiosis and cryptosporidiosis in sheep and goats. Vet Clin North Am Food Anim Pract 6:655–669PubMedGoogle Scholar
  16. Githigia SM, Munyua WK, Kanyari PW (1992) Prevalence of Eimeria species in goats from parts of Central Kenya. Bull Anim Prod Afr 40:283–285Google Scholar
  17. Gul A (2007) The prevalence of Eimeria species in goats in Igdir. Turk J Vet Anim Sci 31:411–414Google Scholar
  18. Harper CK, Penzhorn BL (1999) Occurrence and diversity of coccidia in indigenous, Saanen and crossbred goats in South Africa. Vet Parasitol 82:1–9PubMedCrossRefGoogle Scholar
  19. Jalila A, Dorny P, Sani R, Salim NB, Vercruysse J (1998) Coccidiosis infections of goats in Selangor, peninsular Malaysia. Vet Parasitol 74:165–172PubMedCrossRefGoogle Scholar
  20. Jolley WR, Bardsley KD (2006) Ruminant coccidiosis. Vet Clin Food Anim 22:613–621CrossRefGoogle Scholar
  21. Jubb KVF, Kennedy PC, Palmer N (2007) Pathology of domestic animals, Vol. 2, 5th edn. Academic Press Inc, pp 264–267Google Scholar
  22. Khodakaram-Tafti A, Mansourian M (2008) Pathologic lesions of naturally occurring coccidiosis in sheep and goats. Comp Clin Pathol 17:87–91CrossRefGoogle Scholar
  23. Kimbita EN, Silayo RS, Mwega ED, Mtau AT, Mroso JB (2009) Studies on the Eimeria of Goats at Magadu Dairy Farm SUA, Morogoro, Tanzania. Trop Anim Health Prod 41:1263–1265PubMedCrossRefGoogle Scholar
  24. Koudela B, Bokova A (1998) Coccidiosis in goats in the Czech Republic. Vet Parasitol 76:261–267PubMedCrossRefGoogle Scholar
  25. Levine ND, Ivens V (1970) The coccidian parasite (protozoa, sporozoa) of ruminants. Illinois Biological Monograph 44, University of Illinois press, p 278Google Scholar
  26. Liu Q, Kong F, Ying P (1993) Ultrastructural studies on the endogeneous II. The forming of Macrogamete and the wall of oocyst. Acta Zoologica Sinica 39:343–347Google Scholar
  27. Mehlhorn H (2001) Encyclopedic reference of parasitology, 2nd edn. Springer, BerlinCrossRefGoogle Scholar
  28. Mouafo AN, Richard F, Entzeroth R (2000) Observation of sutures in the oocyst wall of Eimeria tenalla (Apicomplexa). Parasitol Res 86:1015–1017PubMedCrossRefGoogle Scholar
  29. Mouafo AN, Weck-Heimann A, Dubremetz JF, Entzeroth R (2002) Monoclonal antibodies specie for the two types of wall-forming bodies of Eimeria tenella macrogametes (Coccidia, Apicomplexa). Parasitol Res 88:217–224PubMedCrossRefGoogle Scholar
  30. Razavi SM, Hassanvand A (2007) A survey on prevalence of different Eimeria species in goats in Shiraz suburbs. J Fac Vet Med Univ Tehran 61:373–376Google Scholar
  31. Sayin F, Dincer S, Milli U (1980) The life cycle and pathogenicity of Eimeria arloingi (Marotel, 1905) Martin, 1909, in Angora kids and an attempt at its transmission to lambs. Zentrabl Vet Med (B) 27:382–397CrossRefGoogle Scholar
  32. Valentine BA, Cebra CK, Taylor GH (2007) Fatal gastrointestinal parasitism in goats: 31 cases 2001–2006. J Am Vet Med Assoc 231:1098–1103PubMedCrossRefGoogle Scholar
  33. Zachary JF, McGavin MD (2012) Pathologic basis of veterinary disease, 5th edn. Elsevier Mosby, London, pp 28–29Google Scholar
  34. Zaman V, Colley FC (1975) Light and electron microscopic observations of the life cycle of Sarcocystis orientalis sp. in the rat (Rattus norvegicus) and the Malaysian reticulated python (Python reticulates). Z Parasitenkd 47:169–185PubMedCrossRefGoogle Scholar
  35. Zhou K, Wang Y, Chen M et al (2006) Eimeria tenella: further studies on the development of the oocyst. Exp Parasitol 113:174–178CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Mohammad Hashemnia
    • 1
  • Azizollah Khodakaram-Tafti
    • 1
  • Seyed Mostafa Razavi
    • 1
  • Saeed Nazifi
    • 2
  1. 1.Department of Pathobiology, School of Veterinary MedicineUniversity of ShirazShirazIran
  2. 2.Department of Clinical Studies, School of Veterinary MedicineUniversity of ShirazShirazIran

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