Abstract
To estimate the glomerular filtration rate (GFR) in horses, an optimum dose of the nonionic contrast medium iodixanol as a tracer was assessed with blood-sample times. Iodixanol was administered intravenously at 10–40 mg I/kg to geldings and mares, and blood was collected 30, 60, 90, 120, 150, and 180 min later. Serum iodixanol concentration was determined by high-performance liquid chromatography (HPLC), and serum urea nitrogen (UN) and creatinine concentrations were also measured. The combination of 20 mg I/kg iodixanol and sampling times of 60, 90, and 120 min after injection was considered to be appropriate for practical use. In clinically healthy horses, the reference values were determined to be 1.90 ± 0.03 ml/min/kg (150.8 ± 2.94 ml/min/m2), consistent with historical data using different tracers. The result suggests that serum clearance of iodixanol is a ready-to-use tool for a screening of alterations in the equine GFR, although it is necessary to perform a more longitudinal study using horses with a variety of renal functions.
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Acknowledgements
We thank Dr. Tetsuro Yamashita, Department of Food Science and Biochemistry, Faculty of Agriculture, Iwate University, for his helpful advice and suggestions on measuring serum iodixanol concentration with HPLC.
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None of the authors of this article has a financial or personal relationship with other people or organizations that could inappropriately influence or bias the content of the paper.
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We declare that all experiments comply with the current laws of Japan in which we were performed.
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Satoh, H., Abe, S., Kato, M. et al. Optimum conditions for serum clearance of iodixanol, applicable to the estimation of glomerular filtration rate in horses. Vet Res Commun 35, 463–468 (2011). https://doi.org/10.1007/s11259-011-9485-7
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DOI: https://doi.org/10.1007/s11259-011-9485-7