Veterinary Research Communications

, Volume 35, Issue 6, pp 355–365 | Cite as

Further insights into the characterization of equine adipose tissue-derived mesenchymal stem cells

  • Oksana Raabe
  • Katja Shell
  • Antonia Würtz
  • Christine Maria Reich
  • Sabine Wenisch
  • Stefan Arnhold
Original Article

Abstract

Adipose tissue-derived stem cells (ADSCs) represent a promising subpopulation of adult stem cells for tissue engineering applications in veterinary medicine. In this study we focused on the morphological and molecular biological properties of the ADSCs. The expression of stem cell markers Oct4, Nanog and the surface markers CD90 and CD105 were detected using RT-PCR. ADSCs showed a proliferative potential and were capable of adipogenic and osteogenic differentiation. Expression of Alkaline phosphatase (AP), phosphoprotein (SPP1), Runx2 and osteocalcin (OC) mRNA were positive in osteogenic lineages and peroxisome proliferator activated receptor (Pparγ2) mRNA was positive in adipogenic lineages. ADSCs show stem cell and surface marker profiles and differentiation characteristics that are similar to but distinct from other adult stem cells, such as bone marrow-derived mesenchymal stem cells (BM-MSCs). The availability of an easily accessible and reproducible cell source may greatly facilitate the development of stem cell based tissue engineering and therapies for regenerative equine medicine.

Keywords

Adipose tissue Stem cells Differentiation mRNA expression Tissue engineering 

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Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Oksana Raabe
    • 1
  • Katja Shell
    • 1
  • Antonia Würtz
    • 1
  • Christine Maria Reich
    • 1
  • Sabine Wenisch
    • 1
  • Stefan Arnhold
    • 1
  1. 1.Institute of Veterinary -Anatomy, -Histology and -EmbryologyJustus-Liebig University of GiessenGiessenGermany

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