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Assessment of the role of brine shrimp Artemia in white spot syndrome virus (WSSV) transmission

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Abstract

Challenge tests with Artemia four different development stages (nauplii, metanauplii, pseudoadults and adults) to white spot syndrome virus was carried out by immersion challenge and virus-phytoplankton adhesion route in order to asses the possibility of Artemia acting as a vector of WSSV to penaeid shrimp Litopenaeus vannamei postlarvae. The WSSV succeeded in infecting four stages Artemia, and nested-PCR detection for WSSV revealed positive results to virus-phytoplankton adhesion route. No mass mortalities were observed in penaeid shrimp postlarvae fed with WSSV-positive Artemia which exposed to WSSV by virus-phytoplankton adhesion route, whereas WSSV DNA detected in penaeid shrimp postlarvae by nested-PCR. By contrary, no WSSV-positive was detected in any animal fed with WSSV-negative Artemia. These results indicated that Artemia could serve as a vector in WSSV transmission.

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Acknowledgements

Funding for this research was provided by NSFC Project (30371111), China 863 Program (2002AA648010), the 10th National Five Year Major Program (2001BA505B), China Postdoctoral Science Foundation (20080431221), Shangdong Province Postdoctoral Foundation (200803023), Common Research Foundation for Central Institutions of China (220603023026) and Yellow Sea Fisheries Research Institute Postdoctoral Science Foundation funded project. We would like to thank Prof. Jie Huang (Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences) for presenting the WSSV inoculum. We are indebted to the Mr. Summers for helpful criticism of the manuscript.

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Authors and Affiliations

  1. The Key Laboratory of Mariculture, Ministry of Education Fisheries College, Ocean University of China, Qingdao, 266003, People’s Republic of China

    Jia-Song Zhang, Shuang-Lin Dong, Yun-Wei Dong & Xiang-Li Tian

  2. South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, 510300, People’s Republic of China

    Jia-Song Zhang, Yi-Cheng Cao & Zuo-Jia Li

  3. Department of Life Science, Ludong University, Yantai, 264025, People’s Republic of China

    Dong-Chun Yan

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  1. Jia-Song Zhang
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Correspondence to Shuang-Lin Dong.

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Zhang, JS., Dong, SL., Dong, YW. et al. Assessment of the role of brine shrimp Artemia in white spot syndrome virus (WSSV) transmission. Vet Res Commun 34, 25–32 (2010). https://doi.org/10.1007/s11259-009-9329-x

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