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Identification of three immunodominant regions on leukotoxin protein of Fusobacterium necrophorum

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Abstract

To analyze immunodominant regions of leukotoxin protein of Fusobacterium necrophorum strain H05, a series of truncated forms of leukotoxin gene were expressed in Escherichia coli using the vector pGEX-6p-1 or pPROEX HTa. The results of SDS-PAGE showed the truncated forms PL1, PL2, PL4, and PL5 were expressed in Escherichia coli using the vector pGEX-6p-1, and the truncated forms PL3 was expressed in Escherichia coli using the vector pPROEX HTa. These recombinant proteins were able to react with antisera against Fusobacterium necrophorum strain A25. In five recombinant proteins, the recombinant proteins PL1, PL3 and PL4 as vaccine were able to elicit formation of the better protective effects on mice against infection of Fusobacterium necrophorum strain A25.

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Abbreviations

F. necrophorum :

Fusobacterium necrophorum

LktA:

leukotoxin gene

SDS-PAGE:

sodium dodecyl sulfate polyacrylamide gel electrophoresis

References

  • Abe, P., Lennard, E., Holland, J., 1976. Fusobacterium necrophorum infection in mice as a model for the study of liver abscess formation and induction of immunity, Infection and Immunity, 13, 1473–1478.

    PubMed  CAS  Google Scholar 

  • Conion, P., Hepper, K., Teresa, G., 1977. Evaluation of experimentally induced Fusobacterium necrophorum infections in mice, Infection and Immunity, 15, 510–517.

    Google Scholar 

  • Emery, D., Vaughan, J., Clark, B., Stewart, D., 1986. Virulence determinants of Fusobacterium necrophorum and their prophylactic potentialin animals, Australian veterinary journal, 12, 267–274.

    Google Scholar 

  • Garcia, M., Alexander, D.C., McKay, K., 1975. Biological characterization of Fusobacterium necrophorum cell fractions in preparation for toxin and immunization studies, Infection and immunity, 11, 606–616.

    Google Scholar 

  • Garcia, M., McKay, K., 1978. Intraperitoneal immunization against necrobacillosis in experimental animals, Canadian journal of comparative medicine, 42, 121–127.

    PubMed  CAS  Google Scholar 

  • Li, Y., 2000. New technologies and experiences of animal footrot disease, Contemporary livestock, 4, 5–26. In Chinese.

    Google Scholar 

  • Nagaraja, T., Lechtenberg, K., 2007. Liver abscesses in feedlot cattle, The Veterinary clinics of North America. Food animal practice, 23, 351–369. doi:10.1016/j.cvfa.2007.05.002

    Article  PubMed  CAS  Google Scholar 

  • Narayanan, S., Chengappa, M., Stewart, G., Nagaraja, T., 2003. Immunogenicity and protective effects of truncated recombinant leukotoxin proteins of Fusobacterium necrophorum in mice, Veterinary microbiology, 93, 335–347. doi:10.1016/S0378-1135(03)00045-2

    Article  PubMed  CAS  Google Scholar 

  • Narayanan, S., Nagaraja, T., Chengappa, M., Stewart, G., 2001. Cloning, sequencing, and expression of the leukotoxin gene from Fusobacterium necrophorum, Infection and Immunity, 69, 5447–5455. doi:10.1128/IAI.69.9.5447-5455.2001

    Article  PubMed  CAS  Google Scholar 

  • Saginala, S., Nagaraja, T., Lechtenberg, K., Chengappa, M., Kemp, K., Hine, P., 1997. Effect of Fusobacterium necrophorum leukotoxin vaccine on susceptibility to experimentally induced liver abscesses in cattle, Journal of animal science, 75, 1160–1166.

    PubMed  CAS  Google Scholar 

  • Smith, G., Thornton, E., 1993. Pathogenicity of Fusobacterium necrophorum strains from man and animals, Epidemiology and Infection, 110, 499–506.

    Article  PubMed  CAS  Google Scholar 

  • Tan, Z., Nagaraja, T., Chengappa, M., 1996. Fusobacterium necrophorum infections: virulence factors, pathogenic mechanism and control measures, Veterinary research communications, 20, 113–140. doi:10.1007/BF00385634

    Article  PubMed  CAS  Google Scholar 

  • Tan, Z., Nagaraja, T., Chengappa, M., Smith, J., 1994. Biological and biochemical characterization of Fusobacterium necrophorum leukotoxin, American journal of veterinary research, 55, 515–521.

    PubMed  CAS  Google Scholar 

Download references

Acknowledgments

This work was supported by a grant from Heilongjiang Nongkenzongju scientific and technological project (HNKXIV-08-01d), and School Start Fund of Heilongjiang August First Land Reclamation University.

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Authors and Affiliations

  1. College of Animal Science and Technology, Heilongjiang August First Land Reclamation University, Daqing High-tech Industrial Development Zone, Daqing, 163319, People’s Republic of China

    D. B. Sun, R. Wu, G. L. Li, J. S. Zheng, X. P. Liu, Y. C. Lin & D. H. Guo

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  1. D. B. Sun
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  2. R. Wu
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  3. G. L. Li
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  4. J. S. Zheng
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  5. X. P. Liu
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  6. Y. C. Lin
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  7. D. H. Guo
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Correspondence to R. Wu or D. H. Guo.

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Sun, D.B., Wu, R., Li, G.L. et al. Identification of three immunodominant regions on leukotoxin protein of Fusobacterium necrophorum . Vet Res Commun 33, 749–755 (2009). https://doi.org/10.1007/s11259-009-9223-6

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  • DOI: https://doi.org/10.1007/s11259-009-9223-6

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