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Colloid in the anterior pituitary of helmet guinea fowl (Numida meleagris galeata): Morphometric analysis and pattern of occurrence in relation to apoptosis

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Abstract

Colloidal accumulations in the pars distalis of helmet guinea fowls at various ages from 1 to 450 days were examined by Periodic acid-Schiff reaction, immunohistochemistry and electron microscopy. Round, ovoid and elongated colloids were observed. Colloids (69.5 ± 2.997) with 0.169 ± 0.014 µm mean diameter were already present in a 1-day-old bird. Numerous colloids were encountered in 450 days old birds (2931.333 ± 29.847) with 2.263 ±  0.078 µm mean diameter of round colloids. A significant difference in the mean colloidal number and diameter between young and adult birds was observed. In young birds (aged 1–30 days) both Periodic acid-Schiff reaction positive colloids and S-100 positive folliculostellate (FS) cells were found to appear first on or near the posterolateral region. In adult birds, FS cells were found to completely surround the colloids. We examined the biochemical components of colloids and the relationship with apoptosis by immunohistochemistry. Results showed that the colloids are composed of clusterin protein. Apoptotic cells detected by single stranded DNA (ssDNA) were abundant and localized preferentially near colloids. To define clearly the type of cells undergoing apoptosis in the anterior pituitary, we performed electron microscopy. Numerous endocrine cells at different stages of apoptosis were found engulfed by FS cells that were in close association with the colloidal accumulations. The occurrence of extremely large number of colloids in relation to apoptotic profiles in anterior pituitary of helmet guinea fowl is discussed.

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References

  • Anthony, E.L., Gustafson, A.W., 1984. A quantitative study of pituitary colloid in the bat Myotis lucifugus lucifugus in relation to age, sex, and season. Am. J. Anat., 169, 89–100. doi:10.1002/aja.1001690108

    Article  PubMed  CAS  Google Scholar 

  • Baird, A., Mormede, P., Ying, S.Y., Wehrenberg, W.B., Ueno, N., Ling, N., Guillemin, R., 1985. A nonmitogenic pituitary function of fibroblast growth factor: regulation of thyrotropin and prolactin secretion. Proc. Natl. Acad. Sci. U. S. A., 82, 5545–5549. doi:10.1073/pnas.82.16.5545

    Article  PubMed  CAS  Google Scholar 

  • Bassett, E.G., 1951. The anterior lobe of the cattle pituitary II. Distribution of colloid. J. Endocrinol., 7, 215–222. doi:10.1677/joe.0.0070215

    Article  PubMed  CAS  Google Scholar 

  • Benjamin M. 1981. Cysts (large follicles) and colloid in pituitary glands. Gen. Comp. Endocrinol., 45, 425–445. doi:10.1016/0016–6480(81)90046–0

    Article  PubMed  CAS  Google Scholar 

  • Ceccatelli, S., Hulting, A.L., Zhang, X., Gustafsson, L., Villar, M., Hokfelt, T., 1993. Nitric oxide synthase in the rat anterior pituitary gland and the role of nitric oxide in regulation of luteinizing hormone secretion. Proc. Natl. Acad. Sci. USA., 90, 11292–11296 doi:10.1073/pnas.90.23.11292

    Article  PubMed  CAS  Google Scholar 

  • Ciocca, D.R., Gonzalez, C.B., 1978. The pituitary cleft of the rat: an electron microscopic study. Tissue Cell, 10, 725–733. doi:10.1016/0040–8166(78)90058–7

    Article  PubMed  CAS  Google Scholar 

  • Ciocca, D.R., Puy, L.A., Stati, A.O., 1984. Constitution and behavior of follicular structures in the human anterior pituitary gland. Am. J. Pathol., 115, 165–174.

    PubMed  CAS  Google Scholar 

  • Correr, S., Motta, P.M., 1985. A scanning electron-microscopic study of "supramarginal cells" in the pituitary cleft of the rat. Cell Tissue Res., 241, 275–281. doi:10.1007/BF00217171

    Article  PubMed  CAS  Google Scholar 

  • Curé, M., Gerry, H., Girod, C., 1971. Observations sur les structures pseudo-folliculaires du lobe antérieur de l’hypophyse chez plusieurs espêces de Mammifêres et chez l’Homme. C. S. Soc. Biol. (Paris), 165, 1616–1669.

    Google Scholar 

  • Ferrara, N., Henzel, W.J, 1989. Pituitary follicular cells secrete a novel heparin-binding growth factor specific for vascular endothelial cells. Biochem. Biophys. Res. Commun., 161, 851–858. doi:10.1016/0006–291X(89)92678–8

    Article  PubMed  CAS  Google Scholar 

  • Ferrara, N., Schweigerer, L., Neufeld, G., Mitchell, R., Gospodarowicz, D, 1987. Pituitary follicular cells produce basic fibroblast growth factor. Proc. Natl. Acad. Sci. USA., 84, 5773–5777. doi:10.1073/pnas.84.16.5773

    Article  PubMed  CAS  Google Scholar 

  • Ferrara, N., Winer, J., Henzel, W.J., 1992. Pituitary follicular cells secrete an inhibitor of aortic endothelial cell growth: identification as leukemia inhibitory factor. Proc. Natl. Acad. Sci. USA., 89, 698–702. doi:10.1073/pnas.89.2.698

    Article  PubMed  CAS  Google Scholar 

  • Fukuda, T., 1973. Agranular stellate cells (so-called follicular cells) in human fetal and adult adenohypophysis and in pituitary adenoma. Virchows. Arch. A. Pathol. Anat., 359, 19–30. doi:10.1007/BF00549080

    Article  PubMed  CAS  Google Scholar 

  • Haggi, E.S., Torres, A.I., Maldonado, C.A., Aoki, A. Regression of redundant lactotrophs in rat pituitary gland after cessation of lactation. J. Endocrinol., 1986, 111:367–373. doi:10.1677/joe.0.1110367

    Article  PubMed  CAS  Google Scholar 

  • Hanke, H.H., Charriper, A., 1948. The anatomy and cytology of the pituitary gland of the golden hamster (Cricetus autarus). Anat. Rec., 102, 123–139. doi:10.1002/ar.1091020110

    Article  PubMed  CAS  Google Scholar 

  • Horvath, E., Kovacs, K., Penz, G., Ezrin, C., 1974. Origin, possible function and fate of "follicular cells" in the anterior lobe of the human pituitary. Am. J. Pathol., 77, 199–212.

    PubMed  CAS  Google Scholar 

  • Jin, L., Burguera, B.G., Couce, M.E., Scheithauer, B.W., Lamsan, J., Eberhardt, N.L., Kulig, E., Lloyd, R.V., 1999. Leptin and leptin receptor expression in normal and neoplastic human pituitary: evidence of a regulatory role for leptin on pituitary cell proliferation. J. Clin. Endocrinol. Metab., 84, 2903–2911. doi:10.1210/jc.84.8.2903

    Article  PubMed  CAS  Google Scholar 

  • Kagayama, M., 1965. The follicular cell in the pars distalis of the dog pituitary gland: an electron microscope study. Endocrinology, 77, 1053–1060.

    Article  PubMed  CAS  Google Scholar 

  • Kaiser, U.B., Lee, B.L., Carroll, R.S., Unabia, G., Chin, W.W., Childs, G.V., 1992. Follistatin gene expression in the pituitary: localization in gonadotropes and folliculostellate cells in diestrous rats. Endocrinology, 130, 3048–3056. doi:10.1210/en.130.5.3048

    Article  PubMed  CAS  Google Scholar 

  • Kameda, Y., 1990. Occurrence of colloid-containing follicles and ciliated cysts in the hypophysial pars tuberalis from guinea pigs of various ages. Am. J. Anat., 188, 185–198. doi:10.1002/aja.1001880208

    Article  PubMed  CAS  Google Scholar 

  • Kameda, Y., 1991. Occurrence of colloid-containing follicles in the pars distalis of pituitary glands from aging guinea pigs. Cell Tissue Res., 263, 115–124. doi:10.1007/BF00318406

    Article  PubMed  CAS  Google Scholar 

  • Kirkman, H., 1937. A cytological study of the anterior hypophysis of the guinea pig and statistical analysis of its cell types. Am. J. Anat., 61, 233–287. doi:10.1002/aja.1000610204

    Article  Google Scholar 

  • Kubo, M., Iwamura, S., Haritani, M., Kobayashi, M., 1992. Follicular structures in the hypophysis of pigs. Bull. Nat. Inst. Anim. Health, 98, 9–13.

    Google Scholar 

  • Lloyd, R.V., Jin, L., Qian, X., Zhang, S., Scheithauer, B.W., 1995. Nitric oxide synthase in the human pituitary gland. Am. J. Pathol., 146, 86–94.

    PubMed  CAS  Google Scholar 

  • Mamba, K., Isida, T., Kagabu, S., Makita, T., 1989. Scanning electron microscopic observation of the follicular structure of adenohypophysis in guinea fowl. Biomedical SEM, 18, 83–85.

    Google Scholar 

  • Mohamed, F., Fogal, T., Dominguez, S., Scardapane, L., Guzman, J., Piezzi, R.S., 2000. Colloid in the pituitary pars distalis of viscacha (Lagostomus maximus maximus): ultrastructure and occurrence in relation to season, sex, and growth. Anat. Rec., 258, 252–261. doi:10.1002/(SICI)1097–0185(20000301)258:3<252::AID-AR4>3.0.CO;2-D

    Article  PubMed  CAS  Google Scholar 

  • Nakajima, T., Yamaguchi, H., Takahashi, K., 1980. S100 protein in folliculostellate cells of the rat pituitary anterior lobe. Brain Res., 191, 523–531. doi:10.1016/0006–8993(80)91300–1

    Article  PubMed  CAS  Google Scholar 

  • Nolan, L.A., Kavanagh, E., Lightman, S.L., Levy, A., 1998. Anterior pituitary cell population control: basal cell turnover and the effects of adrenalectomy and dexamethasone treatment. J. Neuroendocrinol., 10, 207–215. doi:10.1046/j.1365–2826.1998.00191.x

    Article  PubMed  CAS  Google Scholar 

  • Ogawa, S., Couch, E.F., Kubo, M., Sakai, T., Inoue, K., 1996. Histochemical study of follicles in the senescent porcine pituitary gland. Arch. Histol. Cytol., 59, 467–478. doi:10.1679/aohc.59.467

    Article  PubMed  CAS  Google Scholar 

  • Ogawa, S., Ishibashi, Y., Sakamoto, Y., Kitamura, K., Kubo, M., Sakai, T., Inoue, K., 1997. The glycoproteins that occur in the colloids of senescent porcine pituitary glands are clusterin and glycosylated albumin fragments. Biochem. Biophys. Res. Commun., 234, 712–718. doi:10.1006/bbrc.1997.6704

    Article  PubMed  CAS  Google Scholar 

  • Oishi, Y., Okuda, M., Takahashi, H., Fujii, T., Morii, S., 1993. Cellular proliferation in the anterior pituitary gland of normal adult rats: influences of sex, estrous cycle, and circadian change. Anat. Rec., 235, 111–120. doi:10.1002/ar.1092350111

    Article  PubMed  CAS  Google Scholar 

  • Richardson, B.A., 1980. The pars distalis of the female California leaf-nosed bat, Macrotus californicus, and its possible role in delayed development. Doctoral thesis, University of Arizona.

  • Selye, H., 1943. Experiments concerning the mechanism of pituitary colloid secretion. Anat. Rec., 86, 109–119. doi:10.1002/ar.1090860109

    Article  Google Scholar 

  • Spagnoli, H.H., Charipper, H.A., 1955. The effect of aging on the histology and cytology of the pituitary gland of the golden hamster (Cricetus autarus) with brief reference to simultaneous changes in the thyroid and testis. Anat. Rec., 121, 117–139. doi:10.1002/ar.1091210202

    Article  PubMed  CAS  Google Scholar 

  • Stokreef, J.C., Reifel, C.W., Shin, S.H., 1986. A possible phagocytic role for folliculo-stellate cells of anterior pituitary following estrogen withdrawal from primed male rats. Cell Tissue Res., 243, 255–261. doi:10.1007/BF00251039

    Article  PubMed  CAS  Google Scholar 

  • Vanha-Pertulla, T., Arstila, A.U., 1970. On the epithelium of the rat pituitary residual lumen. Z. Zellforsch. Mikrosk. Anat., 108, 487–500. doi:10.1007/BF00339655

    Article  Google Scholar 

  • Vankelecom, H., Matthys, P., Van Damme, J.V., Heremans, H., Billiau, A., Denef, C., 1993. Immunocytochemical evidence that S-100-positive cells of the mouse anterior pituitary contain interleukin-6 immunoreactivity. J. Histochem. Cytochem., 41, 151–156.

    PubMed  CAS  Google Scholar 

  • Vila-Porcile, E., 1972. The network of the folliculo-stellate cells and the follicles of the adenohypophysis in the rat (pars distalis). Z. Zellforsch. Mikrosk. Anat., 129, 328–369. doi:10.1007/BF00307293

    Article  PubMed  CAS  Google Scholar 

  • Yin, P., Arita, J., 2000. Differential regulation of prolactin release and lactotrope proliferation during pregnancy, lactation and the estrous cycle. Neuroendocrinol., 72, 72–79. doi:10.1159/000054574

    Article  CAS  Google Scholar 

  • Yoshimura, F., Soji, T., Kiguchi, Y., 1977. Relationship between the follicular cells and marginal layer cells of the anterior pituitary. Endocrinol. Jpn., 24, 301–305.

    PubMed  CAS  Google Scholar 

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Acknowledgement

We are grateful to the Japanese ministry of education, culture, sports, science and technology for supporting this study.

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Authors and Affiliations

  1. Department of Veterinary Anatomy, Faculty of Veterinary Medicine, Sokoine University of Agriculture, P.O. Box 3016, Morogoro, Tanzania

    Claudius Luziga, Maulilio John Kipanyula & Gabriel Mbassa

  2. Department of Veterinary Science, Faculty of Agriculture, Yamaguchi University, 753–8515, Yamaguchi, Japan

    Mamba Koichi

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  1. Claudius Luziga
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  2. Maulilio John Kipanyula
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  4. Mamba Koichi
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Correspondence to Claudius Luziga.

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Luziga, C., Kipanyula, M.J., Mbassa, G. et al. Colloid in the anterior pituitary of helmet guinea fowl (Numida meleagris galeata): Morphometric analysis and pattern of occurrence in relation to apoptosis. Vet Res Commun 33, 681–691 (2009). https://doi.org/10.1007/s11259-009-9217-4

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  • DOI: https://doi.org/10.1007/s11259-009-9217-4

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