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Evaluation of serum cystatin-C in dogs with visceral leishmaniasis

Veterinary Research Communications volume 33, pages 529–534 (2009)Cite this article

Abstract

Serum Cystatin C (sCys-C) is one of the most important serum markers of renal function assessment in dogs. The purpose of this study was to determine the sCys-C concentration in dogs with visceral leishmaniasis (VL). In the study, 16 dogs with VL and 10 clinical healty dogs (control) were used. Mean sCys-C concentration of the infected dogs was significantly higher than that of the control group (p < 0.05). Mean serum creatinine concentration was lower and mean blood urea nitrogen, albumin and globulin concentrations were higher in dogs with VL; however, these changes were not statistically significant. Mean total protein and phosphorus concentrations were found to be higher in dogs with VL than healthy dogs (p < 0.05). No significant correlation had been determined between sCys-C and other variables. Visceral leishmaniasis in dogs has increased sCys-C concentration indicating a possible renal impairment; however, further studies are needed to be performed together with renal biopsies in the investigation sCys-C in dogs with VL.

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Abbreviations

CanVL:

canine visceral leihmaniasis

SCys-C:

serum cystatin-c

BUN:

blood urea nitrogen

TP:

total protein

P:

phosphorus

IFAT:

immunoflluoresence antibody assay test

ELISA:

enzyme linked immunosorbent assay

References

  • Abranches P., Silva-Pereira M.C., Conceicao-Silva F.M., Santos-Gomes G.M., Janz J.G., 1991. Canine leishmaniasis: pathological and ecological factors influencing transmission of infection. The Journal of Parasitology, 77, 557–561. doi:10.2307/3283159

    PubMed  Article  CAS  Google Scholar 

  • Almy F.S., Christopher M.M., King D.P., Brown S.A., 2002. Evaluation of cystatin C as an endogenous marker of glomerular filtration rate in dogs. Journal of Veterinary Internal Medicine, 16, 45–51. doi:10.1892/0891-6640(2002)016<0045:EOCCAA>2.3.CO;2

    PubMed  Article  Google Scholar 

  • Antognoni M.T., Siepi D., Porciello F., Fruganti G., 2005. Use of serum cystatin C determination as a marker of renal function in the dog. Veterinary Research Communications, 29 Suppl. 2, 265–267. doi:10.1007/s11259-005-0058-5

    PubMed  Article  Google Scholar 

  • Antognoni M.T., Siepi D., Porciello F., Rueca F., Fruganti G., 2007. Serum cystatin C evaluation in dogs affected by different diseases associated or not with renal insufficiency. Veterinary Research Communications, 31 Suppl. 1, 269–271. doi:10.1007/s11259-007-0044-1

    PubMed  Article  Google Scholar 

  • Braun J.P., Perxachs A., Pechereau D., De La Farge F., 2002. Plasma cystatin C in the dog: reference values and variations with renal failure. Comperative Clinical Pathology, 11, 44–49. doi:10.1007/s580-002-8081-2

    Article  CAS  Google Scholar 

  • Ciaramella P., Oliva G., De Luna R., Grandoni L., Ambrosio R., Cortese L., Scalone A., Persechino A., 1997. A retrospective clinical study of canine leishmaniasis in 150 dogs naturally infected by Leishmania infantum. Veterinary Record, 141, 539–543.

    PubMed  CAS  Google Scholar 

  • Herget-Rosenthal S., Marggraf G., Hüsing J., Göring F., Pietruck F., Janssen O., Philipp T., Kribben A., 2004. Early detection of acute renal failure by serum cystatin C. Kidney International, 66, 1115–1122. doi:10.1111/j.1523-1755.2004.00861.x

    PubMed  Article  CAS  Google Scholar 

  • Jensen A.L., Bomholt M., Moe L., 2001 Preliminary evaluation of a particle-enhanced turbidimetric immunoassay (PETIA) for the determination of serum cystatin C-like immunoreactivity in dogs. Veterinary Clinical Pathology, 30, 86–90. doi:10.1111/j.1939-165X.2001.tb00263.x

    PubMed  Article  Google Scholar 

  • Koutinas A.F., Polizopoulou Z.S., Saridomichelakis M.N., Argyriadis D., Fytianou A., Plevraki K.G., 1999 Clinical considerations on canine visceral leishmaniasis in Greece: a retrospective study of 158 cases (1989–1996). Journal of the American Animal Hospital Association, 35, 376–383.

    PubMed  CAS  Google Scholar 

  • Mares J., Stejskal D., Vavrouskova J., Urbanek K., Herzig R., Hlustik P., 2003. Use of cystatin C determination in clinical diagnostics. Biomedical Papers, 147, 177–180.

    CAS  Google Scholar 

  • Pagitz M., Frommlet F., Schwendenwein I., 2007. Evaluation of biological variance of cystatin C in comparison with other endogenous markers of glomerular filtration rate in healthy dogs. Journal of Veterinary Internal Medicine, 21, 936–942. doi:10.1892/0891-6640(2007)21[936:EOBVOC]2.0.CO;2

    PubMed  Article  Google Scholar 

  • Palacio J., Liste F., Gascón M., 1995. Urinary protein/creatinine ratio in the evaluation of renal failure in canine leishmaniasis. Veterinary Record, 137, 567–568.

    PubMed  CAS  Google Scholar 

  • Palacio J., Liste F., Gascón M., 1997. Enzymuria as an index of renal damage in canine leishmaniasis. Veterinary Record, 140, 477–480.

    PubMed  CAS  Google Scholar 

  • Pasa S., Toz S.O., Voyvoda H., Ozbel Y., 2005. Clinical and serological follow-up in dogs with visceral leishmaniosis treated with allopurinol and sodium stibogluconate. Veterinary Parasitology, 128, 243–249. doi:10.1016/j.vetpar.2004.12.002

    PubMed  Article  CAS  Google Scholar 

  • Poli A., Abramo F., Mancianti F., Nigro M., Pieri S., Bionda A., 1991. Renal involvement in canine leishmaniasis. A light-microscopic, immunohistochemical and electron-microscopic study. Nephron, 57, 444–452.

    PubMed  Article  CAS  Google Scholar 

  • Randers E., Erlandsen E.J., 1999. Serum cystatin C as endogenous marker of the renal function-A Rewiew. Clinical Chemistry Laboratory Medicine, 37, 389–395. doi:10.1515/CCLM.1999.064

    Article  CAS  Google Scholar 

  • Risch L., Blumberg A., Huber A., 1999. Rapid and accurate assessment of glomerular filtration rate in patients with renal transplants using serum cystatin C. Nephrology Dialysis Transplantation, 14, 1991–1996. doi:10.1093/ndt/14.8.1991

    Article  CAS  Google Scholar 

  • Solano-Gallego L., Rodriguez-Cortes A., Trotta M., Zampieron C., Razia L., Furlanello T., Caldin M., Roura X., Alberola J., 2007. Detection of Leishmania infantum DNA by fret-based real-time PCR in urine from dogs with natural clinical leishmaniasis. Veterinary Parasitology, 147, 315–319. doi:10.1016/j.vetpar.2007.04.013

    PubMed  Article  CAS  Google Scholar 

  • Villa P., Jiménez M., Soriano M.C., Manzanares J., Casasnovas P., 2005. Serum cystatin C concentration as a marker of acute renal dysfunction in critically ill patients. Critical Care, 9, 139–143. doi:10.1186/cc3044

    Article  Google Scholar 

  • Xia L.H., Bing X.G., An X.T., 2004. Serum cystatin C assay for the detection of early renal impairment in diabetic patients. Journal of Clinical Laboratory Analysis, 18, 31–35. doi:10.1002/jcla.20005

    PubMed  Article  CAS  Google Scholar 

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Affiliations

  1. Department of Internal Medicine, Faculty of Veterinary Medicine, Adnan Menderes University, Aydin, Turkey

    S. Pasa, G. Bayramli & A. Atasoy

  2. Department of Biochemistry, Faculty of Medicine, Adnan Menderes University, Aydin, Turkey

    A. Karul

  3. Department of Parasitology, Faculty of Medicine, Adnan Menderes University, Aydin, Turkey

    S. Ertug

  4. Department of Parasitology, Faculty of Medicine, Ege University, Izmir, Turkey

    S. Ozensoy Toz

Authors
  1. S. Pasa
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  2. G. Bayramli
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  3. A. Atasoy
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  4. A. Karul
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  5. S. Ertug
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  6. S. Ozensoy Toz
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Correspondence to S. Pasa.

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Pasa, S., Bayramli, G., Atasoy, A. et al. Evaluation of serum cystatin-C in dogs with visceral leishmaniasis. Vet Res Commun 33, 529–534 (2009). https://doi.org/10.1007/s11259-008-9200-5

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  • DOI: https://doi.org/10.1007/s11259-008-9200-5

Keywords

  • Visceral leishmaniasis
  • Dog
  • Serum cystatin C
  • Renal failure