Abstract
Oxidative stress parameters; thiobarbituric acid reaction substances (RBC-TBARS), catalase (RBC-CAT) and reduced glutathione (RBC-GSH)) and the intraerythrocytic concentrations of electrolytes; sodium and potassium (RBC-Na and RBC-K) were determined in 18 well- controlled (WC) and 22 poorly-controlled diabetic mellitus (DM). Dogs with DM had significant higher blood glucose concentration (P < 0.001), haemoglobin A1c (P < 0.01) and fructosamine (P < 0.001) compared to normal healthy dogs (n = 19). Diabetic dogs in both groups had higher RBC-CAT (P < 0.05) while RBC-TBARS were higher significantly only in poorly-controlled DM group (P < 0.05). The RBC-K was significantly higher in both DM groups (P < 0.001). No changes in RBC-GSH and RBC-Na were found between DM and control healthy dogs. By linear regression analysis, the relationship were found between degree of diabetic mellitus and RBC-CAT, RBC-TBARS, RBC-Na and RBC-K. The relationship was also found between oxidative stress parameters and intraerythrocytic K+. The results suggest that in diabetic dogs, oxidative stress occurs which related to the severity of disease and may affect potassium homeostasis.
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Abbreviations
- DM:
-
diabetic mellitus
- HbA1c:
-
Haemoglobin A1c
- RBC-CAT:
-
red blood cell catalase
- RBC-GSH:
-
red blood cell glutathione
- RBC-K:
-
red blood cell potassium
- RBC-Na:
-
red blood cell sodium
- RBC-TBARS:
-
red blood cell thiobarbituric acid reactive substances
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Acknowledgements
This study was supported by the Faculty of Veterinary Science, Chulalongkorn University. We thank Ms. Siripen Komonvanich for her advice in preparing ghost red cell membrane.
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Chansaisakorn, W., Sriphavatsarakorn, P., Sopakdittapong, P. et al. Oxidative stress and intraerythrocytic concentrations of sodium and potassium in diabetic dogs. Vet Res Commun 33, 67–75 (2009). https://doi.org/10.1007/s11259-008-9073-7
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DOI: https://doi.org/10.1007/s11259-008-9073-7