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Pharmacokinetics and pharmacodynamics of chlorpyrifos in male and female cattle after topical administration

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Abstract

The aim of this work was to study the pharmacokinetic behaviour and the inhibitory effect on acetylcholinesterase and butyrylcholinesterase activities of chlorpyrifos in male and female cattle after pour-on administration. Determination of cholinesterase activity in plasma and erythrocyte was carried out according to Ellman kinetic method. The mean baseline activities were 9338.39 ± 1331.61 and 13220.69 ± 2274.18 to acetylcholinesterase and 624.65 ± 39.32 and 641.68 ± 88.08 IU/L to butyrylcholinesterase in females and males, respectively. Acetylcholinesterase was the predominant form of cholinesterase analyzed, with low levels of butyrylcholinesterase. The basal acetylcholinesterase activities of the bulls were significantly greater than those of cows. The inhibitory effect of topical chlorpyrifos administration was lower on butyrylcholinesterase than on acetylcholinesterase. Chlorpyrifos peak plasma concentration (male:10.920 ± 4.18; female:12.12 ± 3.88 μg/L) were reached at 11.92 ± 9.19 and 8.17 ± 7.67 h in male and female, respectively. The values of area under curve were 185.96 ± 168.45 and 278.89 ± 270.00 μg·h/L and mean residence time were 13.95 ± 8.10 and 14.90 ± 9.80 h in male and female, respectively.

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Abbreviations

AChE:

acetylcholinesterase

AUC:

area of the plasma concentration-time curve

AUMC:

area under the first moment curve

BChE:

butyrylcholinesterase

Cmax:

peak plasma concentration

MRT:

mean residence time

tmax:

time of Cmax

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Acknowledgements

The authors wish to thank Mr. Roman Folli, Mr. Ariel Antonelli and Mr. Martín Re for his technical assistance, Mr. Santiago Cano for his advices and the staff of the Servicio de Préstamo Interbibliotecario Facultad de Veterinaria for helping.

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Authors and Affiliations

  1. Cátedra de Farmacología, Facultad de Ciencias Veterinarias, Universidad Nacional del Litoral, R.P. Kreder 2805, Esperanza, 3080, Argentina

    Eduardo J. Picco, Marcelo R. Rubio & Diego C. Díaz David

  2. Cátedra de Farmacología, Dpto. Farmacología y Toxicología, Facultad de Veterinaria, Universidad Complutense de Madrid, Avda. Puerta de Hierro s.n., Madrid, 28040, Spain

    Casilda Rodríguez

  3. Cátedra de Farmacología, Facultad de Ciencias Agropecuarias, Universidad Católica de Córdoba, Camino a Alta Gracia km 7 1/2, Córdoba, 5017, Argentina

    Juan C. Boggio

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  1. Eduardo J. Picco
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  2. Marcelo R. Rubio
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  3. Diego C. Díaz David
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  4. Casilda Rodríguez
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Correspondence to Casilda Rodríguez.

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Picco, E.J., Rubio, M.R., Díaz David, D.C. et al. Pharmacokinetics and pharmacodynamics of chlorpyrifos in male and female cattle after topical administration. Vet Res Commun 32, 401–410 (2008). https://doi.org/10.1007/s11259-008-9035-0

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  • DOI: https://doi.org/10.1007/s11259-008-9035-0

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