Skip to main content
SpringerLink
Log in

Assessment of Lactate Dehydrogenase, Alkaline Phosphatase and Aspartate Aminotransferase Activities in Cow’s Milk as an Indicator of Subclinical Mastitis

  • Published:
Veterinary Research Communications Aims and scope Submit manuscript

Abstract

This study examined the activities of lactate dehydrogenase (LDH), alkaline phosphatase (ALP) and aspartate aminotransferase (AST) in the milk of lactating Holstein cows in association with subclinical mastitis (SCM). A total of 94 milk samples were collected from 58 lactating dairy cows representing stages of lactation from the second to the tenth week after calving. Those which were classified as positive by California mastitis test (CMT) were deemed to have subclinical mastitis. All the milk samples were skimmed by centrifugation at 10 000g at 0°C and were used for enzyme activities estimations. The mean activities of LDH and ALP were higher in the milk from udders with SCM than in the milk from healthy udders p < 0.05). There were no significant differences in AST values. The maximum agreement rates between the CMT results and LDH and ALP values were seen at thresholds of >180 IU/L and >40 IU/L respectively (κ values 0.65 and 0.79, respectively). However, the sensitivity of the tests for identifying SCM at these thresholds was higher for ALP (96.4%) than for LDH (68.5%). In this study, LDH and ALP tests were standardized for cow's milk and results showed that only the ALP test was reliable in the early diagnosis of subclinical mastitis.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

LDH:

lactate dehydrogenase

ALP:

alkaline phosphatase

AST:

aspartate aminotransferase

SCM:

subclinical mastitis

CMT:

California mastitis test

SCC:

somatic cell count

PPV:

positive predictive value

NPV:

negative predictive value

References

  • Batavani, R.A., Mortaz, E., Falahian, K. and Dawoodi, M.A., 2003. Study on frequency, etiology and some enzymatic activities of subclinical ovine mastitis in Urmia, Iran. Small Ruminant Research, 50, 45–50

    Article  Google Scholar 

  • Bergmeyer, H.U., 1974. Method of Enzymatic Analysis (Academic Press, London)

    Google Scholar 

  • Bogin, E. and Ziv, G., 1973. Enzymes and minerals in normal and mastitic milk. Cornell Veterinarian, 63, 666–676

    PubMed  CAS  Google Scholar 

  • Bogin, E., Ziv, G., Avidar, J., Rivetz, B., Gordin, S. and Saran, A., 1977. Distribution of lactate dehydrogenase isoenzymes in normal and inflamed bovine udders and milk. Research in Veterinary Science, 22, 198–200

    PubMed  CAS  Google Scholar 

  • Cabaud, P.G. and Wroblewski, F., 1958. Colorimetric measurement of lactic dehydrogenase activity of body fluids. American Journal of Clinical Pathology, 30, 234–236

    PubMed  CAS  Google Scholar 

  • Csapo, J., Csapo-Kiss, Z. and Stefler, J., Martin, T.G. and Nemethy, S., 1995. Influence of mastitis on D-amino acid content of milk. Journal of Dairy Science, 78, 2375–2381

    PubMed  CAS  Google Scholar 

  • Deianov, M., 1983. LDH activity and isoenzyme spectrum in the leukocytes of cows with catarrhal mastitis and chronic endometritis. Vetrinarno Meditsinski Nauki, 20, 47–51

    CAS  Google Scholar 

  • Donovan, G.A., Risco, C.A. and Shearer, K., 1992. Assessment of the mammary system. Veterinary Clinics of North America: Food Animal Practice, 8, 361–372

    PubMed  CAS  Google Scholar 

  • Honkanen-Buzalski, T. and Sandhom, M., 1981. Trypsin inhibitors in mastitic milk and colostrums: correlation between trypsin inhibitor capacity, bovine serum albumin and somatic cell content. Journal of Dairy Research, 48, 213–233

    PubMed  CAS  Google Scholar 

  • Kaneene, J.B. and Hurd, H.S., 1990. The National Animal Health Monitoring System in Michigan. III. Cost estimates of selected dairy cattle diseases. Preventive Veterinary Medicine, 8, 127–140

    Article  Google Scholar 

  • Kato, K., Mori, K. and Katon, N., 1989. Contribution of leukocyte origin of lactate dehydrogenase isozymes in milk of bovine mastitis. Nippon Juigaku Zasshi. The Japanese Journal of Veterinary Science, 51, 530–539

    PubMed  CAS  Google Scholar 

  • Kitchen, B.J., 1981. Review of the progress of dairy science: bovine mastitis: milk compositional changes and related diagnostic tests. Journal of Dairy Research, 48, 167–188

    Article  PubMed  CAS  Google Scholar 

  • Leslie, K.E., Jansen, J.T. and Lim, G.H., 2002. Opportunities and implications for improved on-farm cowside diagnostics. Proceedings of the DeLaval Hygiene Symposium, 147–160

  • Lightner, J.K., Miller, G.Y., Hueston, W.D. and Dorn, C.R., 1988. Estimation of the costs of mastitis, using National Animal Health Monitoring System and milk somatic cell count. Journal of the American Veterinary Medical Association, 192, 1410–1413

    PubMed  CAS  Google Scholar 

  • Mattila, T., Saari, S., Vartiala, H. and Sandholm, M., 1985. Milk antitrypsin as a marker of bovine mastitis: correlation with bacteriology. Journal of Dairy Science, 68, 114–122

    PubMed  CAS  Google Scholar 

  • Michel, G., 1979. Histochemical behavior of succinate dehydrogenase and lactate dehydrogenase as well as ribonucleic acid in the epithelium of lactic ducts and alveoli of cow udder. Archiv für Experimentelle Veterinarmedizin, 33, 745–751

    PubMed  CAS  Google Scholar 

  • Miller, G.Y., Bartlett, P.C., Lance, S.E., Anderson, J. and Heider, L.E., 1993. Costs of clinical mastitis and mastitis prevention in dairy herds. Journal of the American Veterinary Medical Association, 202, 1230–1236

    PubMed  CAS  Google Scholar 

  • Oliszewski, R., Nunez De Kairuz, M.S., Gonzalez De Elias, S.N. and Oliver, G., 2002. Assessment of β-glucuronidase levels in goat's milk as an indicator of mastitis: comparison with other mastitis detection methods. Journal of Food Protection, 65, 864–866

    PubMed  CAS  Google Scholar 

  • Petrie, A. and Watson, P., 1999. Statistics for Veterinary and Animal Science (Blackwell, London)

    Google Scholar 

  • Poutrel, B. and Rainard, P., 1981. California mastitis test guide of selective dry cow therapy. Journal of Dairy Science, 64, 241–248

    Article  PubMed  CAS  Google Scholar 

  • Reitman, S. and Frankel, S., 1957. A colorimetric method for determination of serum glutamic oxaloacetic and glutamic pyruvic transaminase. American Journal of Clinical Pathology, 28, 56–63

    PubMed  CAS  Google Scholar 

  • Schalm, O.W., Carroll, E.J. and Jain, N.C., 1971. Bovine Mastitis (Lea and Febiger, Philadelphia)

    Google Scholar 

  • Schnyder, J. and Baggiolini, M., 1978. Secretion of lysosomal hydrolases by stimulated and non-stimulated macrophages. Journal of Experimental Medicine, 148, 435–439

    Article  PubMed  CAS  Google Scholar 

  • Symons, D.B. and Wright, L.J., 1974. Changes in bovine mammary gland permeability after intramammary exotoxin infusion. Journal of Comparative Pathology, 84, 9–17

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Theriogenology Section, Department of Clinical Science, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran

    H. Babaei & M. M. Molaei

  2. Department of Food Hygiene, Faculty of Veterinary Medicine, Shahid Bahonar University, Kerman, Iran

    L. Mansouri-Najand

  3. School of Veterinary Medicine, Lorestan University, Khorram Abad, Iran

    A. Kheradmand

Authors
  1. H. Babaei
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. L. Mansouri-Najand
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. M. M. Molaei
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. A. Kheradmand
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. M. Sharifan
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to H. Babaei.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Babaei, H., Mansouri-Najand, L., Molaei, M.M. et al. Assessment of Lactate Dehydrogenase, Alkaline Phosphatase and Aspartate Aminotransferase Activities in Cow’s Milk as an Indicator of Subclinical Mastitis. Vet Res Commun 31, 419–425 (2007). https://doi.org/10.1007/s11259-007-3539-x

Download citation

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11259-007-3539-x

Keywords

Search

Navigation