Skip to main content

Differentiation of Avian Pasteurella multocida Strains by Single-Primer PCR

This is a preview of subscription content, access via your institution.

Abbreviations

AFLP:

amplified fragment length polymorphism

BHI:

brain--heart infusion

dNTP:

deoxynucleotide triphosphate

Pm :

Pasteurella multocida

PCR:

polymerase chain reaction

RAPD:

randomly amplified polymorphic DNA

REA:

restriction endonuclease analysis

References

  1. Biswas, A., Shivachandra, S.B., Saxena, M.K., Kumar, A.A., Singh, V.P. and Srivastava, S.K., 2004. Molecular variability among strains of Pasteurella multocida isolated from an outbreak of haemorrhagic septicaemia in India. Veterinary Research Communications, 28(4), 287–298

    PubMed  Article  CAS  Google Scholar 

  2. Blackall, P.J. and Miflin, J.K., 2000 Identification and typing of Pasteurella multocida: a review. Avian Pathology, 29, 271–287

    Article  CAS  PubMed  Google Scholar 

  3. Blackall, P.J., Fegan, N., Chew, G.T. and Hampson, D.J., 1998. Population structure and diversity of avian isolates of Pasteurella multocida from Australia. Microbiology, 144, 279–289

    PubMed  CAS  Article  Google Scholar 

  4. Chaslus-Dancla, E., Lesage-Descauses, H.C., Leroy-Setrin, S., Martel, J.L., Coudert, P. and Lafont, J.P., 1996. Validation of random amplified DNA assays by ribotyping as tools for epidemiological surveys of Pasteurella from animals. Veterinary Microbiology, 52, 91–102

    PubMed  Article  CAS  Google Scholar 

  5. Dziva, F., Christensen, H., Olsen, J.E. and Mohan, K., 2001. Random amplification of polymorphic DNA and phenotypic typing of Zimbabwean isolates of Pasteurella multocida. Veterinary Microbiology, 82, 361–372

    Google Scholar 

  6. Gautam, R., Kumar, A.A., Singh, V.P., Singh, V.P, Dutta, T.K. and Shivachandra, S.B., 2004. Specific identification of Pasteurella multocida serogroup-A isolates by PCR assay. Research in Veterinary Science, 76(3), 179–185

    Google Scholar 

  7. Gunawardana, G.A., Townsend, K.M. and Frost, A.J., 2000. Molecular characterization of avian Pasteurella multocida isolates from Australia and Vietnam by REP-PCR and PFGE. Veterinary Microbiology, 72, 97–109

    PubMed  Article  CAS  Google Scholar 

  8. Heddleston K.L., 1976. Physiologic characteristics of 1268 cultures of Pasteurella multocida. American Journal of Veterinary Research, 37, 745–747

    PubMed  CAS  Google Scholar 

  9. Huber, B.S., Allred. D.V., Carmen, J.C., Frame, D.D., Whiting, D.G., Cryan, J.R., Olson, T.R., Jackson, P.J., Hill, K., Laker, M.T. and Robison, R.A., 2002. Random amplified polymorphic DNA and amplified fragment length polymorphism analysis of Pasteurella multocida isolates from fatal fowl cholera infections. Journal of Clinical Microbiology, 40(6), 2163–2168

    PubMed  Article  CAS  Google Scholar 

  10. Hunter, P.R. and Gaston, M.A., 1988. Numerical index of the discriminatory ability of typing systems: an application of Simpson’s index of diversity. Journal of Clinical Microbiology, 26, 2465–2466

    PubMed  CAS  Google Scholar 

  11. Kumar, A.A., Shivachandra, S.B., Biswas, A., Singh, V.P., Singh, V.P. and Srivastava, S.K., 2004. Prevalent serotypes of Pasteurella multocida isolated from different animal and avian species in India. Veterinary Research Communications, 28(8), 657–667

    PubMed  Article  CAS  Google Scholar 

  12. Mutters, R., Ihm, P., Pohl, S., Frederiksen, W. and Mannheim, W., 1985. Reclassification of the genus Pasteurella Trevisan 1887 on the basis of deoxyribonucleic acid homology, with proposals for the new species Pasteurella dagmatis, Pasteurella canis, Pasteurella stomatis, Pasteurella anatis and Pasteurella langaa. International Journal of Systemic Bacteriology, 35, 309–322

    Google Scholar 

  13. Olive, D.M. and Bean, P., 1999. Principles and applications of methods for DNA based typing of microbial organisms: a mini review. Journal of Clinical Microbiology, 37(6), 1661–1669

    PubMed  CAS  Google Scholar 

  14. Rhoades, K.R. and Rimler, R.B., 1987. Capsular serogroups of Pasteurella multocida isolated from avian hosts. Avian Diseases, 31, 895–898

    PubMed  Article  CAS  Google Scholar 

  15. Rimler, R.B. and Glisson, J.R., 1997. Fowl cholera. In: B.W. Calnek, H.J.Barnes, C.W. Beard, L.R. McDougald and Y.M. Saif (eds), Diseases of Poultry, 10th edn, (State University Press, Ames, IA), 143–159

  16. Saxena, M.K., Kumar, A.A., Chaudhuri, P., Shivachandra, S.B., Singh, V.P, Singh, V.P. and Sharma, B., 2005. Ribotyping of Indian isolates of Pasteurella multocida based on 16S and 23S rRNA genes. Veterinary Research Communications, 29(6), 527–535

    PubMed  Article  CAS  Google Scholar 

  17. Shivachandra, S.B., Kumar, A.A., Gautam, R., Singh, V.P., Chaudhuri, P. and Srivastava, S.K., 2004a. PCR assay for rapid detection of Pasteurella multocida serogroup-A in morbid tissue materials from experimentally induced fowl cholera in chicks. The Veterinary Journal, 168(3), 349–352

    Google Scholar 

  18. Shivachandra, S.B., Kumar, A.A., Biswas, A., Ramakrishnan, M.A., Singh,V.P. and Srivastava, S.K., 2004b. Antibiotic sensitivity patterns among Indian strains of avian Pasteurella multocida. Tropical Animal Health and Production, 36(8), 743–750

  19. Shivachandra, S.B., Kumar, A.A., Gautam. R., Saxena, M.K., Chaudhuri, P. and Srivastava, S.K., 2005. Detection of multiple strains of Pasteurella multocida in fowl cholera outbreaks by PCR based typing. Avian Pathology, 34(6), 456–462

    PubMed  Article  CAS  Google Scholar 

  20. Shivachandra, S.B., Kumar, A.A., Gautam. R., Singh, V.P., Saxena, M.K. and Srivastava, S.K., 2006a. Identification of avian strains of Pasteurella multocida in India by conventional and PCR assays. The Veterinary Journal (in press)

  21. Shivachandra, S.B., Kumar, A.A., Gautam. R., Joseph, S., Saxena, M.K., Chaudhuri, P. and Srivastava, S.K., 2006b. Characterization of avian strains of Pasteurella multocida by restriction endonuclease and amplified fragment length polymorphism. Research in Veterinary Science, 81(1), 8–18

    Google Scholar 

  22. Shivachandra, S.B., Kumar, A.A., Saxena, M.K., Singh, V.P. and Srivastava, S.K., 2006c. Biochemical characterization of avian strains of Pasteurella multocida in India. Indian Journal of Animal Sciences, 76(6), 429–432

    Google Scholar 

  23. Snipes, K.P., Hirsh, D.C., Kasten, R.W., Carpenter, T.E., Hird, D.W. and McCapes, R.H., 1990. Homogeneity of characteristics of Pasteurella multocida isolated from turkeys and wildlife in California, 1985–1988. Avian Diseases, 34, 315–320

    PubMed  Article  CAS  Google Scholar 

  24. Townsend, K.M., Boyce, J.D., Chung, J.Y., Frost, A.J. and Adler, B., 2001. Genetic organisation of Pasteurella multocida cap loci and development of a multiplex capsular PCR typing system. Journal of Clinical Microbiology, 39(3), 924–929

    PubMed  Article  CAS  Google Scholar 

  25. Welsh, J. and McClelland, M., 1990. Fingerprinting of genomes using PCR with arbitrary primers. Nucleic Acids Research, 18, 7213–7218

    PubMed  Article  CAS  Google Scholar 

  26. Williams, J.G.K., Kubeli, A.R., Livak, K.J., Rafalsk, J.A. and Tingey, S.V., 1990. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Research, 18, 6531–6535

    PubMed  Article  CAS  Google Scholar 

  27. Wilson, K.,1987. Preparation of genomic DNA from bacteria. In: F.M. Ausubal, R. Brent, R.L. Kirston, D.D. Moore, J.G. Seidman, J.A. Smith and K. Struhe (eds), Current Protocols in Molecular Biology, vol. 1, (Wiley, New~York), 241–242

  28. Wilson, M.A., Morgan, M.J. and Barger, G.E., 1993. Comparison of DNA fingerprinting and serotyping for identification of avian Pasteurella multocida isolates. Journal of Clinical Microbiology, 31, 255–259

    PubMed  CAS  Google Scholar 

Download references

Author information

Affiliations

Authors

Corresponding author

Correspondence to S. B. Shivachandra.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Shivachandra, S.B., Kumar, A.A. & Chaudhuri, P. Differentiation of Avian Pasteurella multocida Strains by Single-Primer PCR. Vet Res Commun 31, 941–949 (2007). https://doi.org/10.1007/s11259-007-0102-8

Download citation

Keywords

  • avian
  • Pasteurella multocida
  • PCR
  • single primer
  • RAPD