Kocan, K.M., de la Fuente, J. and Blouin, E.F., 2007. Targeting the tick/pathogen interface for developing new anaplasmosis vaccine strategies. Veterinary Research Communications, 31(Suppl. 1), 91–96
Abstract
Bovine anaplasmosis is a tick-borne hemolytic disease of cattle that occurs worldwide caused by the intraerythrocytic rickettsiae Anaplasma marginale. Control measures, including use of acaricides, administration of antibiotics and vaccines, have varied with geographic location. Our research is focused on the tick-pathogen interface for development of new vaccine strategies with the goal of reducing anaplasmosis, tick infestations and the vectorial capacity of ticks. Toward this approach, we have targeted (1) development of an A. marginale cell culture system to provide a non-bovine antigen source, (2) characterization of an A. marginale adhesion protein, and (3) identification of key tick protective antigens for reduction of tick infestations. A cell culture system for propagation of A. marginale was developed and provided a non-bovine source of A. marginale vaccine antigen. The A. marginale adhesion protein, MSP1a, was characterized and use of recombinant MSP1a in vaccine formulations reduced clinical anaplasmosis and infection levels in ticks that acquired infection on immunized cattle. Most recently, we identified a tick-protective antigen, subolesin, that reduced tick infestations, as well as the vectorial capacity of ticks for acquisition and transmission of A marginale. This integrated approach to vaccine development shows promise for developing new strategies for control of bovine anaplasmosis.
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Blouin E.F., de la Fuente J., Garcia-Garcia J.C., Sauer J.R., Saliki J.T. and Kocan K.M., 2002. Applications of a cell culture system for studying the interaction of Anaplasma marginale with tick cells. Animal Health Research Review, 3, 57–68
Blouin E.F., Saliki J.T., de la Fuente J., Garcia-Garcia J.C. and Kocan K.M., 2003. Antibodies to Anaplasma marginale major surface proteins 1a and 1b inhibit infectivity for cultured tick cells. Veterinary Parasitology, 111, 247–260
Brown W.C., Palmer G.H., Lewin H.A. and McGuire T.C., 2001. CD4(+) T lymphocytes from calves immunized with Anaplasma marginale major surface protein 1 (MSP1), a heteromeric complex of MSP1a and MSP1b, preferentially recognize the MSP1a carboxyl terminus that is conserved among strains. Infection and Immunity, 69, 6853–6862
de la Fuente J., Garcia-Garcia J.C., Blouin E.F. and Kocan K.M., 2002a. Infection of tick cells and bovine erythrocytes with the intracellular ehrlichia Anaplasma marginale excludes infection with other genotypes. Journal of Clinical and Diagnostic Laboratory Immunology, 9, 658–668
de la Fuente J., Kocan K.M., Garcia-Garcia J.C., Blouin E.F., Claypool P.L. and Saliki J.T., 2002b. Vaccination of cattle with Anaplasma marginale derived from tick cell culture and bovine erythrocytes followed by challenge-exposure by ticks. Veterinary Microbiology, 89, 239–251
de la Fuente J., Blouin E.F. and Kocan K.M., 2003a. Infection exclusion of the rickettsial pathogen Anaplasma marginale in the tick vector Dermacentor variabilis. Clinical and Diagnostic Laboratory Immunology, 10, 182–184
de la Fuente J., Kocan K.M., Garcia-Garcia J.C., Blouin E.F., Halbur T. and Onet V., 2003b. Immunization against Anaplasma marginale major surface protein 1a reduces infectivity for ticks. Journal of Applied Research in Veterinary Medicine, 1, 285–292
de la Fuente J., Lew A., Lutz H., Meli M.L., Hofmann-Lehmann R., Shkap V., Molad T., Mangold A.J., Almazán C., Naranjo V., Gortázar C., Torina A., Caracappa S., García-Pérez A.L., Barral M., Oporto B., Ceci L., Carelli G., Blouin E.F. and Kocan K.M., 2005. Genetic diversity of Anaplasma species major surface proteins and implications for anaplasmosis serodiagnosis and vaccine development. Animal Health Research Reviews, 6, 75–89
de la Fuente J. and Kocan, K.M., 2006. Strategies for development of vaccines for control of ixodid tick species. Parasite Immunology, 28, 275–283
de la Fuente J., Almazán C., Blouin E.F., Naranjo V. and Kocan K.M., 2006. Reduction of tick infections with Anaplasma marginale and A. phagocytophilum by targeting the tick protective antigen subolesin. Parasitology Research, In press
Garcia-Garcia J.C., de la Fuente J., Kocan K.M., Blouin E.F., Halbur T., Onet V.C. and Saliki J.T., 2004a. Mapping of B-cell epitopes in the N-terminal repeated peptides of the Anaplasma marginale major surface protein 1a and characterization of the humoral immune response of cattle immunized with recombinant and whole organism antigens. Veterinary Immunology and Immunopathology, 98, 137–151
Garcia-Garcia J.C., de la Fuente J., Bell-Eunice G., Blouin E.F. and Kocan K.M., 2004b. Glycosylation of major surface protein 1a and its putative role in adhesion of Anaplasma marginale to tick cells. Infection and Immunity, 72, 3022–3030
Kocan K.M., Halbur T., Blouin E.F., Onet V., de la Fuente J., Garcia-Garcia J. and Saliki J.T., 2001. Immunization of cattle with Anaplasma marginale derived from tick cell culture. Veterinary Parasitology, 102, 151–161
Kocan K.M., de la Fuente J., Guglielmone A.A. and Melendéz R.D., 2003. Anaplasma marginale: Antigens and control alternatives for a rickettsial hemoparasite of cattle. Clinical Microbiology Reviews, 16, 698–712
Kocan K.M., de la Fuente J., Blouin E.F. and Garcia-Garcia J.C., 2004. Anaplasma marginale (Rickettsiales: Anaplasmataceae): recent advances in defining host-pathogen adaptations of a tick-borne rickettsia. Parasitology, 129, S285–S300
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Kocan, K.M., de la Fuente, J. & Blouin, E.F. Targeting the Tick/Pathogen Interface for Developing New Anaplasmosis Vaccine Strategies. Vet Res Commun 31 (Suppl 1), 91–96 (2007). https://doi.org/10.1007/s11259-007-0070-z
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DOI: https://doi.org/10.1007/s11259-007-0070-z