Abstract
The aim was to study the relationship between the infection rate of Eperythrozoon suis and the status of the immune system. Four pigs with chronic eperythrozoonosis were divided into experimental and control groups. Immunosuppression of the experimental group was accomplished by injecting with high-dose glucocorticoid (dexamethasone) on 3 consecutive days. Microscopic examination of blood smears was performed to observe the change of infection rate after administration. The blood samples of each group were collected on day 7 after administration for half-nested PCR assay. The microscopic results showed that the infection rate of the experimental group rose distinctly by 40 h late and remained at 90% in the days following administration. No changes were observed in the control group. PCR results showed a single expected 250 bp fragment in each group, which validated the positive microscopic results. This study demonstrated the significant effect of dexamethasone (DEX) on Eperythrozoon infection.
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Abbreviations
- E. suis :
-
Eperythrozoon suis
- DEX:
-
dexamethasone
- PCR:
-
polymerase chain reaction
References
Berent, L.M. and Messick, J.B., 2003. Physical map and genome sequencing survey of Mycoplasma haemofelis (Haemobartonella felis). Infection and Immunity, 71, 3657–3662
Hall, S.M., Cipriano, J.A., Schoneweis, D.A., Smith, J.E. and Fenwick, B.W., 1988. Isolation of infective and non-infective Eperythrozoon suis bodies from the whole blood of infected swine. Veterinary Record, 123, 651
Henderson, J.P., O’Hagan, J., Hawe, S.M. and Pratt, M.C.H., 1997. Anaemia and low viability in piglets infected with Eperythrozoon suis. The Veterinary Record, 140, 144–146
Hoelzle, L.E, Adelt, D., Hoelzle, K., Heinritzi, K. and Wittenbrink, M.M., 2003. Development of a diagnostic PCR assay based on novel DNA sequences for the detection of Mycoplasma suis (Eperythrozoon suis) in porcine blood. Veterinary Microbiology, 93, 185–196
Isobe, T. and Lillehoj, H.S., 1993. Dexamethasone suppresses T cell-mediated immunity and enhances disease susceptibility to Eimeria mivati infection. Veterinary Immunology and Immunopathology, 39, 431–446
Kawashima, K., Tsunemitsu, H., Horino, R., Katsuda, K., Onodera, T., Shoji, T., Kubo, M., Haritani, M. and Murakami, Y., 2003. Effects of dexamethasone on the pathogenesis of porcine circovirus type 2 infection in piglets. Journal of Comparative Pathology, 129, 294–302
Kunicka, J.E., Talle, M.A., Denhardt, G.H., Brown, M., Prince, L.A. and Goldstein, G., 1993. Immunosuppression by glucocorticoids: inhibition of production of multiple lymphokines by in vivo administration of dexamethasone. Cell Immunology, 149, 39–49
Messick, J.B., 2004. Hemotrophic mycoplasmas (hemoplasmas): a review and new insights into pathogenic potential. Veterinary Clinical Pathology, 33, 2–13
Messick, J.B., Berent, L.M. and Cooper, S.K., 1998. Development and evaluation of a PCR-based assay for detection of Haemobartonella felis in cats and differentiation of H. felis from related bacteria by restriction fragment length polymorphism analysis. Journal of Clinical Microbiology, 36, 462–466
Messick, J.B., Cooper, S.K. and Huntley, M., 1999. Development and evaluation of a polymerase chain reaction assay using the 16S rRNA gene for detection of Eperythrozoon suis infection. Journal of Veterinary Diagnostic Investigation, 11, 229–236
Neimark, H., Johansson, K.E., Rikihisa Y. and Tully, J.G., 2001. Proposal to transfer some members of the genera Haemobartonella and Eperythrozoon to the genus Mycoplasma with descriptions of Candidatus Mycoplasma haemofelis, Candidatus Mycoplasma haemomuris, Candidatus Mycoplasma haemosuis and Candidatus Mycoplasma wenyonii. International Journal of Systematic and Evolutionary Microbiology, 51(Pt 3), 891–899
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Yuan, C., Yang, Z., Zhu, J. et al. Effect of an Immunosuppressor (Dexamethasone) on Eperythrozoon Infection. Vet Res Commun 31, 661–664 (2007). https://doi.org/10.1007/s11259-007-0029-0
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DOI: https://doi.org/10.1007/s11259-007-0029-0