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Veterinary Research Communications

, Volume 31, Issue 6, pp 765–773 | Cite as

Comparative Pharmacokinetics of Gentamicin after Intravenous, Intramuscular, Subcutaneous and Oral Administration in Broiler Chickens

  • E. A. Abu-BashaEmail author
  • N. M. Idkaidek
  • A. F. Al-Shunnaq
Article

Abstract

The pharmacokinetics and bioavailability of gentamicin sulphate (5 mg/kg body weight) were studied in 50 female broiler chickens after single intravenous (i.v.), intramuscular (i.m.), subcutaneous (s.c.) and oral administration. Blood samples were collected at time 0 (pretreatment), and at 5, 15 and 30 min and 1, 2, 4, 6, 8, 12, 24 and 48 h after drug administration. Gentamicin concentrations were determined using a microbiological assay and Bacillus subtillis ATCC 6633 as a test organism. The limit of quantification was 0.2 μg/ml. The plasma concentration–time curves were analysed using non-compartmental methods based on statistical moment theory. Following i.v. administration, the elimination half-life (t 1/2β), the mean residence time (MRT), the volume of distribution at steady state (V ss), the volume of distribution (V d,area) and the total body clearance (ClB) were 2.93 ± 0.15 h, 2.08 ± 0.12 h, 0.77 ± 0.05 L/kg, 1.68 ± 0.39 L/kg and 5.06 ± 0.21 ml/min per kg, respectively. After i.m. and s.c. dosing, the mean peak plasma concentrations (C max) were 11.37 ± 0.73 and 16.65 ± 1.36 μg/ml, achieved at a post-injection times (t max) of 0.55 ± 0.05 and 0.75 ± 0.08 h, respectively. The t 1/2β was 2.87 ± 0.44 and 3.48 ± 0.37 h, respectively after i.m. and s.c. administration. The V d,area and ClB were 1.49 ± 0.21 L/kg and 6.18 ± 0.31 ml/min per kg, respectively, after i.m. administration and were 1.43 ± 0.19 L/kg and 4.7 ± 0.33 ml/min per kg, respectively, after s.c. administration. The absolute bioavailability (F) of gentamicin after i.m. administration was lower (79%) than that after s.c. administration (100%). Substantial differences in the resultant kinetics data were obtained between i.m. and s.c. administration. The in vitro protein binding of gentamicin in chicken plasma was 6.46%.

Keywords

bioavailability pharmacokinetics chicken gentamicin Bacillus subtilis 

Abbreviations

AUC

area under plasma concentration–time curve

ClB

total body clearance

Cmax

maximum plasma concentration

tmax

time to peak concentration

kel

elimination rate

Vss

volume of distribution at steady state

Vd,area

volume of distribution

MRT

mean residence time

t1/2β

elimination half-life

F

absolute bioavailability

MIC

minimum inhibitory concentration

i.v.

intravenous

i.m.

intramuscular

s.c.

subcutaneous

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Copyright information

© Springer Science + Business Media, Inc. 2007

Authors and Affiliations

  • E. A. Abu-Basha
    • 1
    Email author
  • N. M. Idkaidek
    • 2
  • A. F. Al-Shunnaq
    • 1
  1. 1.Department of Veterinary Basic Medical Sciences, Faculty of Veterinary MedicineJordan University of Science and TechnologyIrbidJordan
  2. 2.Department of Pharmaceutical Technology, Faculty of PharmacyJordan University of Science and TechnologyIrbidJordan

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