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Immunohistochemical Study of Phospho-Stat3-ser727 Expression in Feline Mammary Gland Tumours

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Abstract

We describe the expression of pStat3-ser727 (signal transducer and activator of transcription 3 phosphorylated on serine 727) in normal, hyperplastic and neoplastic feline mammary gland tissue assessed by immunohistochemistry in 56 cats. The samples included 4 normal mammary non-lactating tissues, 13 hyperplastic lesions (9 lobular and 4 fibroepithelial) and 39 tumours (6 benign and 33 carcinomas). For immunohistochemistry, tissue sections were incubated with anti-pStat3-ser727 monoclonal antibodies and visualized with EnVision-DAB polymer. pStat3-ser727 positivity was quantified in a semi-quantitative manner, differentiating cytoplasmic and nuclear localization. Intense anti-pStat3-ser727 immunoreactivity was detected in epithelial neoplastic cells and in the fibro-component in two fibroepithelial hyperplastic lesions. The immunostaining was dot-like in the cytoplasm and homogeneous in the nuclei in both benign and malignant lesions. Statistically significant relations were observed between nuclear expression of pStat3-ser727 and the pleomorphism score (p = 0.006), mitotic activity (p < 0.0001), and histological grade (p < 0.0001). In contrast, no significant correlations were observed for cytoplasmic pStat3-ser727. These findings add new and interesting information on the potential role of the phosphorylated form of Stat3 in malignant lesions.

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Abbreviations

BSA:

bovine serum albumin

CSF-1:

colony-stimulating factor-1

DAB:

3,3′-diaminobenzide tetrahydrochloride

EGFr:

epidermal growth factor receptor

IFN:

interferon

FMLP:

N-formyl-methionyl-leucyl-phenylalanine

MAPK:

mitogen-activated protein kinase

PBS:

phosphate-buffered saline

PDGFR:

platelet-derived growth factor receptor

pStat3-ser727:

phosphorylated signal transducer and activator of transcription 3-serine 727

Stat3:

signal transducers and activators of transcription 3

TBS:

Tris-buffered saline

TNF:

tumour necrosis factor

WHO:

World Health Organization

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Authors and Affiliations

  1. Department of Public Health, Comparative Pathology and Veterinary Hygiene School of Veterinary Medicine, University of Padua, Agripolis, Legnaro

    C. Petterino & M. Drigo

  2. Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle D’Aosta, National Reference Centre of Veterinary and Comparative Oncology, Genoa, Italy

    G. Podestà, A. Ratto & C. Pellegrino

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  1. C. Petterino
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  2. G. Podestà
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  3. A. Ratto
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  4. M. Drigo
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  5. C. Pellegrino
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Correspondence to C. Petterino.

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Petterino, C., Podestà, G., Ratto, A. et al. Immunohistochemical Study of Phospho-Stat3-ser727 Expression in Feline Mammary Gland Tumours. Vet Res Commun 31, 173–184 (2007). https://doi.org/10.1007/s11259-006-3330-4

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  • DOI: https://doi.org/10.1007/s11259-006-3330-4

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