Veterinary Research Communications

, Volume 30, Issue 6, pp 679–687 | Cite as

Antemortem Diagnosis of CDV Infection by RT-PCR in Distemper Dogs with Neurological Deficits without the Typical Clinical Presentation

  • A. M. Amude
  • A. A. AlfieriEmail author
  • A. F. Alfieri


In dogs with neurological disturbances without myoclonus and extraneural signs, the clinical diagnosis of distemper is difficult perform. Considering the great infectious potential of the disease, the possibility of carrying out an antemortem diagnosis of distemper is important, particularly in hospitalized patients with neurological disease. The present study was carried out to evaluate RT-PCR for antemortem CDV detection in hospitalized dogs with neurological disturbances without the typical findings of distemper. We investigated five dogs with canine distemper virus (CDV) encephalomyelitis, in which the clinical diagnosis was not performed owing to the absence of characteristic signs of the disease, such as myoclonus and systemic signs. We observed an apparent high sensitivity of RT-PCR in urine samples for detection of CDV: four out of five urine samples were RT-PCR positive. The results of the present study suggest that urine is a good biological sample for antemortem CDV detection by RT-PCR in dogs with distemper encephalomyelitis in which the clinical diagnosis is likely to be difficult owing to the absence of suggestive distemper signs. The use of two different body fluids (urine and CSF) may increase the RT-PCR sensitivity for antemortem diagnosis of distemper in such cases.


dogs distemper canine distemper virus neurological signs RT-PCR 



canine distemper virus


central nervous system


cerebrospinal fluid


Madin-Darby canine kidney




reverse transcription–polymerase chain reaction


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  1. Baumärtner, W., Örrvell, C. and Reinacher, M., 1989. Naturally occurring canine distemper virus encephalitis: distribution and expression of viral polypeptides in nervous tissues. Acta Neuropathologica 78, 504–512CrossRefGoogle Scholar
  2. Boom, R., Sol, C.J.A., Salimans, M.M.M., Jansen, C.L., Werthein-van Dillen, P.M.E. and van der Noordaa, J., 1990. Rapid and simple method for purification of nucleic acids. Journal of Clinical Microbiology 28, 495–503PubMedGoogle Scholar
  3. Braund, K.G., 2001. Inflammatory Diseases of the Central Nervous System. Available at: (Accessed 11 November 2001).
  4. Frisk, A.L., Konig, M., Moritz, A. and Baumgärtner, W., 1999. Detection of canine distemper virus nucleoprotein RNA by reverse transcription-PCR using serum, whole blood, and cerebrospinal fluid from dogs with distemper. Journal of Clinical Microbiology 37, 3634–3643PubMedGoogle Scholar
  5. Gebara, C.M.S., Wosiacki, S.R., Negrão, F.J., Alfieri, A.A. and Alfieri, A.F., 2004a. Lesões histopatológicas no sistema nervoso central de cães com encefalite e diagnóstico molecular da infecção pelo vírus da cinomose canina. Arquivos Brasileiro de Medicina Veterinária e Zootecnia 56, 168–174Google Scholar
  6. Gebara, C.M.S., Wosiacki, S.R., Negrão, F.J., de Oliveira, D.B., Beloni, S.N.B., Alfieri, A.A. and Alfieri, A.F., 2004b. Detecção do gene da nucleoproteína do vírus da cinomose canina por RT-PCR em urina de cães com sinais clínicos de cinomose. Arquivos Brasileiros de Medicina Veterinária e Zootecnia 56, 480–487Google Scholar
  7. Greene, G.E. and Appel, M.J., 1998. Canine distemper. In: G.E. Greene (eds.), Infectious Diseases of the Dog and the Cat, 2nd edn, (W.B. Saunders, Philadelphia), 9–22Google Scholar
  8. Jóźwik, A. and Frymus, T., 2005. Comparison of the immunofluorescence assay with RT-PCR and nested PCR in the diagnosis of canine distemper. Veterinary Research Communications 29, 347–359PubMedCrossRefGoogle Scholar
  9. Kim, Y.H., Cho, K.W., Youn, H.Y., Yoo, H.S. and Han, H.R., 2001. Detection of canine distemper virus through one step RT-PCR combined with nested PCR. Journal of Veterinary Science 2, 59–63PubMedGoogle Scholar
  10. Koutinas, A.F., Polizopoulou, Z.S., Baumgärtner, W., Lekkas, S. and Kontos, V., 2002. Relation of clinical signs to pathological changes in 19 cases of canine distemper encephalomyelitis. Journal of the Comparative Pathology 126, 47–56CrossRefGoogle Scholar
  11. Meertens, N., Stoffel, M.H., Cherpillod, P., Wittek, R., Vandevelde, M. and Zurbriggen, A., 2003. Mechanism of reduction of virus release and cell–cell fusion in persistent canine distemper virus infection. Acta Neuropathologica 106, 303–310PubMedCrossRefGoogle Scholar
  12. Moritz, A., Frisk, A.L. and Baumgärtner, W., 2000. The evaluation of diagnostic procedures for the detection of canine distemper virus infection. European Journal of Companion Animal Practice 10, 37–47Google Scholar
  13. Muller, C.F., Fatzer, R.S., Beck, K., Vandevelde, M. and Zurbriggen, A., 1995. Studies on canine distemper virus persistence in the central nervous system. Acta Neuropathologica 89, 438–445PubMedGoogle Scholar
  14. Okita, M., Yanai, T., Ochikubo, F., Gemma, T., Mori, T., Maseki, T., Yamanouchi, K., Mikami, T. and Kai, C., 1997. Histopathological features of canine distemper recently observed in Japan. Journal of the Comparative Pathology 116, 403–408CrossRefGoogle Scholar
  15. Rzeźutka, A. and Mizak, B., 2002. Application of N-PCR for diagnosis of distemper in dogs and fur animals. Veterinary Microbiology 88, 95–103PubMedCrossRefGoogle Scholar
  16. Saito, T.B., 2001. Padronização da técnica de reação em cadeia pela polimerase (RT-PCR) para o diagnóstico ante post mortem do vírus da cinomose canina (MS thesis, Universidade Estadual de Londrina, Brazil)Google Scholar
  17. Shin, Y., Mori, T., Okita, M., Gemma, T., Kai, C. and Mikami, T., 1995. Detection of canine distemper virus nucleocapsid protein gene in canine peripheral blood mononuclear cells by RT-PCR. Journal of Veterinary Medical Science 57, 439–445PubMedGoogle Scholar
  18. Stettler, M., Beck, K., Wagner, A., Vandevelde, M. and Zurbriggen, A., 1997. Determinants of persistence in canine distemper viruses. Veterinary Microbiology 57, 83–93PubMedCrossRefGoogle Scholar
  19. Summers, B.A. and Appel, M.J., 1994. Aspects of canine distemper virus and measles virus encephalomyelitis. Neuropathology and Applied Neurobiology 20, 525–534PubMedCrossRefGoogle Scholar
  20. Tipold, A., Vandevelde, M. and Jaggy, A., 1992. Neurological manifestation of canine distemper virus infection. Journal of Small Animal Practice 33, 466–470CrossRefGoogle Scholar
  21. Vandevelde, M. and Cachin, M., 1993. The neurologic form of canine distemper. In: J.D. Bonagura and R.W. Kirk (eds.), Kirk's Current Veterinary Therapy XI, Small Animal Practice, (W.B. Saunders, Philadelphia), 1003–1007Google Scholar
  22. Zurbriggen, A., Graber, H.U. and Vandevelde, M., 1995. Selective spread and reduced virus release leads to canine distemper virus persistence in the nervous system. Veterinary Microbiology 44, 281–288PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science + Business Media, Inc. 2006

Authors and Affiliations

  1. 1.Laboratório de Virologia Animal, Departamento de Medicina Veterinária Preventiva (DMVP), Centro de Ciências Agrárias (CCA)Universidade Estadual de LondrinaLondrinaBrazil

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