Skip to main content

Dietary Supplements of Vitamins E and C and β-Carotene Reduce Oxidative Stress in Cats with Renal Insufficiency

Veterinary Research Communications volume 30, pages 403–413 (2006)Cite this article

Abstract

Oxidative stress may contribute to the progression of chronic renal failure. In this study, cats with spontaneous renal insufficiency were fed a dry cat food supplemented with the antioxidants vitamins E and C, and β-carotene for 4 weeks. When compared with healthy cats, cats with renal insufficiency had a tendency to oxidative stress. The antioxidant supplements significantly reduced DNA damage in cats with renal insufficiency as evidenced by reduced serum 8-OHdG and comet assay parameters. Therefore, supplements of vitamins E and C and β-carotene as antioxidants may be beneficial to cats with renal disease.

This is a preview of subscription content, access via your institution.

Access options

Buy single article

Instant access to the full article PDF.

USD 39.95

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Abbreviations

8-OHdG:

8-hydroxy-2'-deoxyguanosine

HPLC:

high-performance liquid chromatography

MDA:

malondialdehyde

ORAC:

oxygen radical absorption capacity

References

  • Allen, T.A., Polzin, D.J. and Adams, L.G., 2000. Renal disease. In: M.S. Hand, C.D. Thatcher, R.L. Remillard and P. Roudebush (eds), Small Animal Clinical Nutrition, 4th edn, (Mark Morris Institute, Topeka, KS), 563–604

    Google Scholar 

  • Beddhu, S., Samore, M.H., Roberts, M.S., Stoddard, G.J., Pappas, L.M. and Cheung, A.K., 2003. Creatinine production, nutrition, and glomerular filtration rate estimation. Journal of the American Society of Nephrology, 14, 1000–1005

    PubMed  Google Scholar 

  • Cao, G., Alessio, H. and Cutler, R.G., 1993. Oxygen-radical absorbency capacity assay for antioxidants. Free Radical Biology and Medicine, 14, 303–311

    Article  CAS  PubMed  Google Scholar 

  • Chew, B.P., Park, J.S., Weng, B.C., Wong, T.S., Hayek, M.G. and Reinhart, G.A., 2000. Dietary β-carotene absorption by blood plasma and leukocytes in domestic cats. Journal of Nutrition, 130, 2322–2325

    CAS  PubMed  Google Scholar 

  • Collins, A., Dusinska, M., Franklin, M., Somorovska, M., Petrovska, H., Duthie, S., Fillion, L., Panayiotidis, M., Raslova, K. and Vaughan, N., 1997. Comet assay in human biomonitoring studies: reliability, validation, and applications. Environmental and Molecular Mutagenesis, 30, 139–146

    Article  CAS  PubMed  Google Scholar 

  • De Rodas, B.Z., Maxwell, C.V., Davis, M.E., Mandali, S., Broekman, E. and Stoecker, B.J., 1998. L-Ascorbyl-2-polyphosphate as a vitamin C source for segregated and conventionally weaned pigs. Journal of Animal Science, 76, 1636–1643

    CAS  PubMed  Google Scholar 

  • DeLange, R.J. and Glazer, A.N., 1989. Phycoerythrin fluorescence-based assay for peroxy radicals: a screen for biologically relevant protective agents. Analytical Biochemistry, 177, 300–306

    Article  CAS  PubMed  Google Scholar 

  • Gunter, E.W., Lewis, B.G. and Koncikowski, S.M., 1996. Laboratory Procedures Used for the Third National Health and Nutrition Examination Survey (NHANES III), 1988–1994, (US Department of Health and Human Services, Centers for Disease Control and Prevention, Atlanta, GA, and National Center for Health Statistics, Hyattsville, MD), VII-F-1–VII-F-12

    Google Scholar 

  • Gwinner, W. and Grone, H.J., 2000. Role of reactive oxygen species in glomerulonephritis. Nephrology Dialysis Transplantation, 15, 1127–1132

    Article  CAS  Google Scholar 

  • Hahn, S., Kuemmerle, N.B., Chan, W., Hisano, S., Saborio, P., Krieg, R.J., Jr. and Chan, J.C., 1998. Glomerulosclerosis in the remnant kidney rat is modulated by dietary α-tocopherol. Journal of the American Society of Nephrology, 9, 2089–2095

    CAS  PubMed  Google Scholar 

  • Harper, E.J., 2000. Antioxidant compositions and method for companion animals. Patent Publication WO00/44375.

  • Hasselwander, O. and Young, I.S., 1998. Oxidative stress in chronic renal failure. Free Radical Research, 29, 1–11

    CAS  PubMed  Google Scholar 

  • Hidiroglou, M., Batra, T.R. and Zhao, X., 1997. Comparison of vitamin C bioavailability after multiple or single oral dosing of different formulations in sheep. Reproduction Nutrition Development, 37, 443–448

    CAS  Google Scholar 

  • Hoehler, D., Frohlich, A.A., Marquardt, R.R. and Stelsovsky, H., 1998. Extraction of alpha-tocopherol from serum prior to reversed phase liquid chromatography. Journal of Agricultural and Food Chemistry, 46, 973–978

    Article  CAS  Google Scholar 

  • Klahr, S., 1997. Oxygen radicals and renal diseases. Mineral and Electrolyte Metabolism, 23, 140–143

    CAS  PubMed  Google Scholar 

  • Nielsen, F., Mikkelsen, B.B., Nielsen, J.B., Andersen, H.R. and Grandjean, P., 1997. Plasma malondialdehyde as biomarker for oxidative stress: reference interval and Effects of life-style factors. Clinical Chemistry, 43, 1209–1214

    CAS  PubMed  Google Scholar 

  • Osawa, T., Yoshida, A., Kawakishi, S., Yamashita, K. and Ochi, H., 1995. Protective role of dietary antioxidants in oxidative stress. In: R.G. Cutler, L. Packer, J. Bertram and A. Mori (eds), Oxidative Stress and Aging (Birkhauser Verlag, Boston), 367–377

    Google Scholar 

  • Peuchant, E., Delmas-Beauvieux, M., Dubourg, L., Thomas, M.J., Perromat, A., Aparicio, M., Clerc, M. and Combe, C., 1997. Antioxidant Effects of a supplemented very low protein diet in chronic renal failure. Free Radical Biology and Medicine, 22, 313–320

    Article  CAS  PubMed  Google Scholar 

  • Schulze, J., Broz, J. and Ludwig, B. 1993. Efficacy of L-ascorbate-2-polyphosphate as a source of ascorbic acid in dogs. International Journal for Vitamin and Nutrition Research, 63, 63–64

    CAS  PubMed  Google Scholar 

  • Schweigert, F.J., Raila, J., Wichert, B. and Kienzle, E., 2002. Cats absorb β-carotene, but it is not converted to vitamin A. Journal of Nutrition, 132, 1610S–1612S

    CAS  PubMed  Google Scholar 

  • Shigenaga, M. and Ames, B., 1991. Assays for 8-hydroxy-2'-deoxyguanosine: a biomarker of in vivo oxidative DNA damage. Free Radical Biology and Medicine, 10, 211–216

    Article  CAS  PubMed  Google Scholar 

  • Singh, N.P., McCoy, M.T., Tice, R.R. and Schneider, E.L., 1988. A simple technique for quantitation of low levels of DNA damage in individual cells. Experimental Cell Research, 175, 184–191

    Article  CAS  PubMed  Google Scholar 

  • Waz, W.R. and Feld, L.G., 1994. Reactive oxygen molecules in the kidney. Advances in Experimental Medicine and Biology, 366, 171–183

    CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Hill's Science and Technology Center, 1035 NE 43rd Street, Topeka, KS, 66617, USA

    S. Yu & I. Paetau-Robinson

Authors
  1. S. Yu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. I. Paetau-Robinson
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to S. Yu.

Additional information

Partial data of this study were presented at the Experimental Biology meeting on 17–21 April, 2004. Washington DC, USA

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Yu, S., Paetau-Robinson, I. Dietary Supplements of Vitamins E and C and β-Carotene Reduce Oxidative Stress in Cats with Renal Insufficiency. Vet Res Commun 30, 403–413 (2006). https://doi.org/10.1007/s11259-006-3269-5

Download citation

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11259-006-3269-5

Keywords

  • antioxidants
  • cats
  • DNA damage
  • oxidative stress
  • renal insufficiency