Abstract
Ten out of 47 calves that were born in a small Brahman herd from southern Brazil developed progressive muscular weakness and tremors, lethargy and poor body condition. Necropsy was performed on three affected animals. The only gross lesion detected was paleness of the muscles of the trunk and limbs. Multiple cytoplasm vacuoles located in different tissues were the principal microscopic lesions. Vacuoles were particularly evident in skeletal muscles and myocardium. PAS-positive granules were numerous in skeletal muscle fibres and Purkinje fibres of the myocardium, but were also observed in the neurons of the brain and spinal cord, and in the vascular smooth muscle fibres from all the examined tissues. Pretreatment with diastase completely abolished the PAS reactivity. The vacuoles reacted strongly to Griffonia simplicifolia II and Concanavalia ensiformes lectins, whose biding pattern has been reported as useful for demonstration of glycogen. Examination of the electron micrographs revealed that glycogen was free within the cytoplasm or accumulated in membrane-bound granules of several tissues, especially in striated muscle, liver and neurons of the CNS. These findings were consistent with generalized glycogenosis.
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Abbreviations
- CNS:
-
central nervous system
- HE:
-
haematoxylin and eosin
- PAS:
-
periodic acid-Schiff
- PBS:
-
phosphate-buffered saline
References
Alroy, J., Orgad, U., Ucci, A.A. and Pereira, M.E.A., 1984. Identification of glycoprotein storage diseases by lectins: a new diagnostic method. Journal of Histochemistry and Cytochemistry, 32, 311–315
Brown, A.M., 2004. Brain glycogen re-awakened. Journal of Neurochemistry, 89, 537–552
Cook, R.D., McC. Howell, J., Dorling, P.R. and Richards, R.B., 1982. Changes in the nervous tissue in bovine generalized glycogenosis type II. Neuropathology and Applied Neurobiology, 8, 95–107
Damjanov, I., 1987. Lectin cytochemistry and histochemistry. Laboratory Investigation, 57, 5–20
Dennis, J.A., Moran, C. and Healy, P.J., 2000. The bovine alpha-glucosidase gene: coding region, genomic structure, and mutations that cause bovine generalized glycogenosis. Mammalian Genome, 11, 206–212
Dennis, J.A., Healy, P.J. and Reichmann, K.G., 2002. Genotyping Brahman cattle for generalized glycogenosis. Australian Veterinary Journal, 80, 286–291
Glew, R.H., Basu, A., Prence, E.M. and Remaley, A.T., 1985. Lysosomal storage diseases. Laboratory Investigation, 53, 250–269
Goldstein, I.J. and Hayes, C.E., 1978. The lectins: carbohydrate binding proteins of plants and animals. Advances in Carbohydrate Chemistry and Biochemistry, 35, 127–340
Goldstein, I.J., Hayes, C.E., Monsigny, M., Osawa, T. and Sharon, N., 1980. What should be called a lectin? Nature, 285, 66
Healy, P.J., Sewell, C.A., Nieper, R.E., Whittle, R.J. and Reichmann, K.G., 1987. Control of generalized glycogenosis in a Brahman herd. Australian Veterinary Journal, 64, 278–280
Hennigar, R.A., Schulte, B.A. and Spicer, S.S., 1986. Histochemical detection of glycogen using Griffonia simplicifolia agglutinin II. Histochemical Journal, 18, 589–596
Howell, J.M., Dorling, P.R., Cook, R.D., Robinson, W.F., Bradley, S. and Gawthorne, J.M., 1981. Infantile and late onset form of generalized glycogenosis type 2 in cattle. Journal Pathology, 134, 266–277
Ito, T., Newkirk, C., Strum, J.M. and McDowell, E.M., 1990. Modulation of glycogen stores in epithelial cells during airway development in Syrian golden hamsters: a histochemical study comparing Concanavalin A binding with the periodic acid–Schij reaction. Journal of Histochemistry and Cytochemistry, 38, 691–697
Jolly R.D., Hartley, W.J. and Path, F.R.C., 1977. Storage disease of domestic animals. Australian Veterinary Journal, 53,1–8
Manktelow, B.W. and Hartley, W.J., 1975. Generalized glycogen storage disease in sheep. Journal of Comparative Pathology, 85, 139–145
Mostafa, I.R., 1970. A case of glycogenic cardiomegaly in a dog. Acta Veterinaria Scandinavica, 11, 197–208
Murakami, H., Takagi, A., Nonaka, I., Ishiura, S., Sugita, H. and Mizutani, M., 1980. Glycogenosis II in Japanese quails. Jikken Dobutsu, 29, 475–478
O'Sullivan, B.M., Healy, P.J., Fraser, I.R., Niepers, R.E, Whittle R.J. and Sewell, C.A., 1981. Generalized glycogenosis in Brahman cattle. Australian Veterinary Journal, 57, 227–229
Pompe, J.C., 1932. Over idiopathische hypertrophie van het hart. Nederlands Tijdschrift voor Geneeskunde, 76, 304–312
Prophet, E.B., Mills, B., Arrington, J.B. and Sobin, L.H., 1992. Laboratory Methods in Histotechnology, (Armed Forces Institute of Pathology; American Registry of Pathology, Washington), 153
Reichmann, K.G., Twist, J.O. and Thistlethwaite, E.J., 1993. Clinical, diagnostic and biochemical features of generalized glycogenosis type II in Brahman cattle. Australian Veterinary Journal, 70, 405–408
Sandstrom, B., Westman, J. and Ockerman, P.A., 1969. Glycogenosis of the central nervous system in the cat. Acta Neuropathologia, 14, 194–200
Spicer, S.S. and Schulte, B.A., 1988. Detection and differentiation of glycoconjugates in various cell types by lectin histochemistry. Basic and Applied Histochemistry, 32, 307–320
Summers, B., Cummings, J.F. and De Lahunta, A., 1995. Veterinary Neuropathology. Degenerative Diseases of the Central Nervous System, (Mosby, St Louis), 208–350
Wisselaar, H.A., Hermans, M.M.P., Visser, W.J, Kroos, M.A., Oostra, B.A., Aspden, W., Harrison, B., Hetzel, D.J.S, Reuser, A.J.J. and Drinkwater, R.D., 1993. Biochemical genetics of glycogenosis type II in Brahman cattle. Biochemical and Biophysical Research Communications, 190, 941–947
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Zlotowski, P., Gimeno, E.J., Diaz, A. et al. Lectin-Histochemistry: Glycogenosis in Cattle. Vet Res Commun 30, 369–377 (2006). https://doi.org/10.1007/s11259-006-3237-0
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DOI: https://doi.org/10.1007/s11259-006-3237-0