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Erythrocyte Lipid Peroxides and Blood Zinc and Copper Concentrations in Acute Undifferentiated Diarrhoea in Calves

  • Biochemistry
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Abstract

Undifferentiated acute calf diarrhoea is a major concern for the dairy industry and its aetiopathogenesis remains diverse. The present study aimed to examine the role of oxidative stress through estimation of erythrocyte lipid peroxide levels and blood zinc and copper concentrations using natural cases of diarrhoea in calves aged 15–30 days old. The calves were kept under identical managemental conditions and were provided with pooled whole colostrum during the first three days and thereafter with only whole milk until they were 1 month old. Diarrhoeic (n = 11) and normal calves (n = 11) of the same age group (15–30 days old) were randomly selected from an organized dairy farm for the study. The mean blood zinc concentration (50.01±2.45 μmol/L vs 66.06±3.06 μmol/L) was significantly (p < 0.05) lower and copper concentration was significantly (p < 0.05) higher in diarrhoeic calves (12.90±0.31 μmol/L vs. 9.44±0.16 μmol/L) than in the healthy calves. The erythrocyte lipid peroxides level was higher (p < 0.05) in diarrhoeic calves (6.88±0.23 nmol malondialdehyde (MDA) per mg of haemoglobin (Hb)) than healthy calves (6.27±0.07 nmol MDA per mg Hb). From the results of the study it is concluded that oxidative stress and antioxidant minerals (zinc and copper) might play important roles in the aetiopathogenesis of bovine calf diarrhoea.

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Abbreviations

E. coli :

Escherichia coli

Hb:

haemoglobin

MDA:

malondialdehyde

NaCl:

sodium chloride

RBC:

red blood cells

References

  • Argenzio, R.A. and Rhoads, J.M., 1997. Reactive oxygen metabolites in the piglets cryptosporidiosis. Pediatric Research, 41, 521–526

    CAS  PubMed  Google Scholar 

  • Baljer, G., Eichhorn, W., Gobel, E., Wolf, F.M. and Bachmann, P.A., 1987. Prevalence and distribution of important diarrhoea pathogens in newborn calves in southern Germany in 1984–1986. Tierarztliche-Umschau, 42, 56–60

    Google Scholar 

  • Black, R.E. and Sazawal, S., 2001. Zinc and childhood infectious disease morbidity and mortality. British Journal of Nutrition, 85, S125–129

    CAS  PubMed  Google Scholar 

  • Brigham, K.L. and Meyrick, B., 1986. Endotoxin and lung injury. American Review of Respiratory Diseases, 133, 913–927

    CAS  Google Scholar 

  • Clemens, M.R. and Waller, H.D., 1987. Lipid peroxidation in erythrocytes. Chemistry and Physics of Lipids, 45, 251–268

    Article  CAS  PubMed  Google Scholar 

  • Conner, J.G., Eckersall, P.D., Doherty, M. and Douglas, T.A., 1986. Acute phase response and mastitis in the cow. Research in Veterinary Science, 41, 126–128

    CAS  PubMed  Google Scholar 

  • Erickson, D.O., Harrold, R.L. and Carlson, R.C., 1981. Calf scours as it relates to selected nutritional components of colostrums. North Dakota Farm Research, 38, 18–20

    Google Scholar 

  • Evans, P. and Halliwell, B., 2001. Micronutrients: oxidant/antioxidant status. British Journal of Nutrition, 85, s57–s74

    Google Scholar 

  • Ghergariu, S. and Kadar, L., 1979. Behaviour of Cu, Fe and Zn blood levels in diseased cattle. I. Changes in neonatal diarrhoea. II. Changes during respiratory disease in young cattle. Zentralblatt für Veterinarmedizin, 26A, 666–675

    Google Scholar 

  • Green, E.M. and Adams, H.R., 1992. New perspectives in circulatory shock: pathophysiologic mediators of the mammalian response to endotoxemia and sepsis. Journal of the American Veterinary Medical Association, 200, 1834–1841

    CAS  PubMed  Google Scholar 

  • Gurer, H., Ozgunes, H., Neal, R., Spitzand, D.R. and Ercal, N., 1998. Antioxidant effects of N-acetyl cysteine and succimer in red blood cells from lead exposed rats. Toxicology, 128, 181–189

    CAS  PubMed  Google Scholar 

  • Halliwell, B., Gutteridge, J.M.C. and Cross, C.E., 1992. Free radicals, antioxidants, and human disease; where are we now? Review article. Journal of Laboratory Clinical Medicine, 119, 598–619

    CAS  Google Scholar 

  • Kolmer, J.A., Spandling, E.H. and Robinson, H.W., 1951. Approved Laboratory Techniques, (Appleton Century Craft, New York), 1090–1091

    Google Scholar 

  • Krebs, N.F., 1998. Zinc metabolism in disease. In: NIH, 1998. Zinc: What Role Might Supplements Play? A Conference, Uniformed Services University of Health Sciences, MD, USA, 6 Nov, 1998, 26–30

  • Naresh, R., Dwivedi, S.K., Swarup, D. and Dey, S., 2001. Zinc, copper and cobalt concentrations in blood during inflammation of mammary gland in dairy cows. Asian Australasian Journal of Animal Sciences, 14, 564–566

    CAS  Google Scholar 

  • Nockels, C.F. and Blair, R., 1996. Antioxidants improve cattle immunity following stress. Animal Feed Science and Technology, 62, 59–68

    Article  CAS  Google Scholar 

  • Oteiza, P.I, Olin, K.L., Fraga, C.G. and Keen, C.L., 1995. Zinc deficiency causes oxidative damage to proteins, lipids and DNA in rat testes. Journal of Nutrition, 125, 823–829

    CAS  PubMed  Google Scholar 

  • Oviedo, M.T., Arya, L.N. and Hernandez, F., 1986. Detection of bacterial, parasitic and viral agents in calves with diarrhoea. Cliencias Veterinarias Costa Ricca, 8, 27–28

    Google Scholar 

  • Patra, R.C., Swarup, D., Dwivedi, S.K. and Sahoo, A., 2001. Trace minerals in blood of young calves during exposure to lead. Indian Journal of Animal Sciences, 71, 507–510

    CAS  Google Scholar 

  • Placer, Z.A., Cushman, L. and Johnson, B., 1966. Estimation of product of lipid peroxidation (malondialdehyde) in biochemical systems. Annals of Biochemistry, 16, 359–364

    Article  CAS  Google Scholar 

  • Radostits, O.M., Gay, C.C., Blood, D.C. and Hinchcliff, K.W., 2000. Veterinary Medicine, 9th edn, (WB Saunders, London)

    Google Scholar 

  • Rowlands, G.J., Little, W., Stark, A.J. and Manston, R., 1979. The blood composition of cows in commercial dairy herds and its relationship with season and lactation. British Veterinary Journal, 135, 64–74

    CAS  PubMed  Google Scholar 

  • Roy, J.H.B., 1980. Factors affecting susceptibility of calves to diseases. Journal of Dairy Science, 63, 650–664

    CAS  PubMed  Google Scholar 

  • Snedecor, G.W. and Cochran, W.G., 1989. Statistical Methods, 8th edn, (Oxford and IBH, New Delhi)

    Google Scholar 

  • Snodgrass, D.R., Terzolo, H.R., Sherwood, D., Campbell, I., Menzies, J.D. and Synge, B.A., 1986. Aetiology of diarrhoea in young calves. The Veterinary Record, 119, 31–34

    CAS  PubMed  Google Scholar 

  • Somashekariaish, B.V, Padmaja, K. and Prasad, A.R., 1992. Lead induced lipid peroxidation and antioxidant defense components of developing chicken embryos. Free Radical Biology, 13, 107–114

    Google Scholar 

  • Utley, H.G., Bernheim, F. and Hochseim, P., 1967. Effect of sulfhydryl reagents on peroxidation of microsomes. Archives of Biochemistry and Biophysics, 118, 29–32

    Article  CAS  Google Scholar 

  • Vankampen, E.J. and Ziglstra, W.G., 1961. Colorimetric determination of haemoglobin. Clinica Chemica Acta, 6, 3588

    Google Scholar 

  • Wernicki, A. and Rzedzicki, J., 1988. Occurrence of some etiopathogenic microorganisms in natural and diarrhoeic calves up to three weeks of age. Medycyna Weterynaryjna, 44, 137–139

    Google Scholar 

  • Wingley, P., Hulme, S.D. and Barrow, P.A., 1999. Phagocytic and oxidative burst activity of chicken thrombocytes to Salmonella, Escherichia coli and other bacteria. Avian Pathology, 28, 567–572

    Google Scholar 

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Authors and Affiliations

  1. Division of Medicine, Indian Veterinary Research Institute, Izatnagar, 243 122, UP

    R. Ranjan, R. C. Patra & D. Swarup

  2. Division of Medicine, Indian Veterinary Research Institute, Nainital, Uttaranchal, India

    R. Naresh

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  1. R. Ranjan
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  2. R. Naresh
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  3. R. C. Patra
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  4. D. Swarup
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Correspondence to R. C. Patra.

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Ranjan, R., Naresh, R., Patra, R.C. et al. Erythrocyte Lipid Peroxides and Blood Zinc and Copper Concentrations in Acute Undifferentiated Diarrhoea in Calves. Vet Res Commun 30, 249–254 (2006). https://doi.org/10.1007/s11259-006-3185-8

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  • DOI: https://doi.org/10.1007/s11259-006-3185-8

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