Abstract
The aim of this study was to develop a new experimental model of Chlamydophila pneumoniae infection in the hamster. Intraperitoneal injection of C. pneumoniae purified elementary bodies (EBs) in the hamsters caused a systemic infection, since it was possible to isolate viable chlamydiae from several organs up to 14 days after infection. In particular, spleen infection was detectable up to 7 days post infection in 100% of animals. In contrast, cultures of the organs obtained from intranasally infected animals were far less frequently positive. Systemic infection probably occurred via macrophages, as demonstrated by the presence of intracellular chlamydial inclusions in peritoneal macrophages of peritoneally inoculated animals four days after infection. Furthermore, by infecting LLC-MK2 cells with supernatant preparations obtained from these macrophages, it was possible to observe the development of chlamydial intra-cytoplasmic inclusions after 96 h. Immunization of 18 hamsters with heat-inactivated purified EBs completely protected 16 animals and substantially reduced infection levels in the remaining two. Sera obtained from immunized hamsters prior to challenge reacted mainly against two C. pneumoniae proteins of about 60 kDa, when tested by immunoblot.
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REFERENCES
Alder, J., Jarvis, K., Mitten, M., Shipkowitz, N.L., Gupta, P. and Clement, J., 1993. Clarithromycin therapy of experimental Treponema pallidum infections in hamsters. Antimicrobial Agents and Chemotherapy, 37, 864–867
Bini, L., Sanchez-Campillo, M., Santucci, A., Magi, B., Marzocchi, B., Comanducci, M., Christiansen, G., Birkelund, S., Cevenini, R., Vretou, E., Ratti, G. and Pallini, V., 1996. Mapping of Chlamydia trachomatis proteins by immobiline-polyacrylamide two-dimensional electrophoresis: spot identification by N-terminal sequencing and immunoblotting. Electrophoresis, 17, 185–190
Campbell, L.A., Rosenfeld, M. and Kuo, C.C., 2000. The role of Chlamydia pneumoniae in atherosclerosis: recent evidence from animal models. Trends in Microbiology, 8, 255–257
Comanducci, M., Manetti, R., Bini, L., Santucci, A., Pallini, V., Cevenini, R., Sueur, J.M., Orfila, J. and Ratti, G., 1994. Humoral immune response to plasmid protein pgp3 in patients with Chlamydia trachomatis infection. Infection and Immunity, 62, 5491–5497
Farencena, A., Comanducci, M., Donati, M., Ratti, G. and Cevenini, R., 1997. Characterization of a new isolate of Chlamydia trachomatis which lacks the common plasmid and has properties of biovar trachoma. Infection and Immunity, 65, 2965–2969
Fong, I.W., Chiu, B., Viira, E., Fong, M.W., Jang, D. and Mahony, J., 1997. Rabbit model for Chlamydia pneumoniae infection. Journal of Clinical Microbiology, 35, 48–52
Fong, I.W., Chiu, B., Viira, E., Jang, D. and Mahony, J.B., 1999. De novo induction of atherosclerosis by Chlamydia pneumoniae in a rabbit model. Infection and Immunity, 67, 6048–6055
Goodman, J.L., Jurkovich, P., Kodner, C. and Jonhson, R.C., 1991. Persistent cardiac and urinary tract infections with Borrelia burgdoferi in experimentally infected Syrian hamsters. Journal of Clinical Microbiology, 29, 894–896
Grayston, J.T., Aldous, M.B., Easton, A., Wang, S.P., Kuo, C.C., Campbell, L.A. and Altman, J., 1993. Evidence that Chlamydia pneumoniae causes pneumonia and bronchitis. Journal of Infectious Diseases, 168, 1231–1235
Laemmli, U.K., 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227, 680–685
Marrie, T.M., 1993. Community acquired pneumonia. Clinical Infectious Diseases, 18, 501–515
Melnick, S.L., Shahar, E., Folsom, R., Grayston, G.T., Sorlie, P.D., Wang, S.P. and Szklo, M., 1993. Past infection by Chlamydia pneumoniae strain: TWAR and asymptomatic carotid atherosclerosis: atherosclerotic risk in communities (ARIC): study investigators. American Journal of Medicine, 95, 499–504
Moazed, T.C, Kuo, C.C, Patton, D.L., Grayston, J.T. and Campbell, L.A., 1996. Experimental rabbit models of Chlamydia pneumoniae infection. American Journal of Pathology, 148, 667–676
Moazed, T.C, Kuo, C.C., Grayston, J.T. and Campbell, L.A., 1997. Murine models of Chlamydia pneumoniae infection and atherosclerosis. Journal of Infectious Diseases, 175, 883–890
Moazed, T.C, Kuo, C.C., Grayston, J.T. and Campbell, L.A., 1998. Evidence of systemic dissemination of Chlamydia pneumoniae via macrophages in the mouse. Journal of Infectious Diseases, 177, 1322–1325
Moroni, A., Pavan, G., Donati, M. and Cevenini, R., 1996. Differences in the envelope proteins of Chlamydia pneumoniae. Chlamydia trachomatis, and Chlamydia psittaci shown by two-dimensional gel electrophoresis. Archives of Microbiology, 165, 164–168
Morrison, R.P. and Caldwell, H.D., 2002. Immunity to murine chlamydial genital infection. Infection and Immunity, 70, 2741–2751
Pal, S., Rangel, J., Peterson, E.M. and de la Maza, L.M., 1999. Immunogenic and protectiv ability of the two developmental forms of Chlamydiae in a mouse model of infertility. Vaccine, 18, 752–761
Saikku, P., Leinonen, M., Tenkanen, L., Linnanmaki, E., Ekman, M.R., Manninen, V., Manttari, M., Frick, M.H. and Huttunen, N.H., 1992. Chronic Chlamydia pneumoniae infection as a risk factor for coronary heart disease in the Helsinki heart study. Annual of Internal Medicine, 116, 273–278
Sambri, V., Marangoni, A., Eyer, C, Reichhuber, C., Soutschek, E., Negosanti, M., D’Antuono, A. and Cevenini, R., 2001. Western immunoblotting with five Treponema pallidum recombinant antigens for serologic diagnosis of syphilis. Clinical and Diagnostic Laboratory Immunology, 8, 534–539
Sambri, V., Marangoni, A., Giacani, L., Gennaro, R., Murgia, R., Cevenini, R. and Cinco, M., 2002. Comparative in vitro activity of five cathelicidin-derived synthetic peptides against Leptospira. Borrelia and Treponema pallidum. Journal of Antimicrobial Chemotherapy, 50, 895–902
Towbin, H., Staehelin, T. and Gordon, J., 1979. Electrophoretic transfer of proteins and nucleic acids from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of Sciences of the United States of America, 76, 4350–4354
Yang, Z.P., Kuo, C.C. and Grayston, J.T., 1993. A mouse model of Chlamydia pneumoniae strain TWAR pneumonitis. Infection and Immunity, 61, 2037–2040
Yang, Z.P., Kuo, C.C. and Grayston, J.T., 1995. Systemic dissemination of Chlamydia pneumoniae following intranasal inoculation in mice. Journal of Infectious Diseases, 171, 736–738
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Marangoni, A., Sambri, V., Donati, M. et al. Development of a hamster model of Chlamydophila pneumoniae infection. Vet Res Commun 29 (Suppl 1), 61–70 (2005). https://doi.org/10.1007/s11259-005-0837-z
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DOI: https://doi.org/10.1007/s11259-005-0837-z