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Influence of the Th2 immune response established by Nippostrongylus brasiliensis infection on the protection offered by different vaccines against Chlamydophila abortus infection

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Abstract

Chlamydophila abortus is the aetiological agent of enzootic abortion in small ruminants in which it infects the placenta to cause abortion during the last trimester of gestation. In a mouse model, a Th1 immune response involving IFN-γ production and CD8+ T cells is necessary for the infection to be resolved. The authors previously demonstrated that infection with Nippostrongylus brasiliensis, a rodent gastrointestinal nematode extensively used in experimental models to induce Th2 responses, alters the specific immune response against C. abortus infection, increasing bacterial multiplication in liver and reducing specific IFN-γ production. The aim of the present work was to clarify whether a Th2 immune response has any influence on the success of vaccination using both inactivated and attenuated vaccines. The results showed that the Th2 response established prior to vaccination did not influence the induction of protection offered by the vaccines. However, the effectiveness of this protective response can be altered, depending on the adjuvant employed in the inactivated vaccines, when the Th2 response is established after vaccination, just before challenge with C. abortus.

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Abbreviations

AH:

aluminium hydroxide vaccine

Con A:

concanavalin A

IFN-γ:

interferon gamma

IFUs:

inclusion forming units

IL-4:

interleukin 4

IL-5:

interleukin 5

Nb-es:

Nippostrongylus brasiliensis excretory-secretory antigens

OEA:

ovine enzootic abortion

PBS:

phosphate-buffered saline

p.i:

post-infection

QS:

QS-21 vaccine

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Authors and Affiliations

  1. Department of Animal Health, Veterinary School of University of Murcia, Spain

    M. R. Caro, N. Ortega, M. C. Gallego, F. Cuello, M. R. Ruiz-Ybañez & J. Salinas

  2. Department of Histopathology, Veterinary School of University of Murcia, Spain

    A. J. Buendía & C. M. Martínez

  3. Center for Infectious Diseases, University of Würzburg, Germany

    K. J. Erb

Authors
  1. M. R. Caro
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  2. A. J. Buendía
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  3. N. Ortega
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  4. M. C. Gallego
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  5. C. M. Martínez
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  6. F. Cuello
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  7. M. R. Ruiz-Ybañez
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  8. K. J. Erb
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  9. J. Salinas
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Corresponding author

Correspondence to J. Salinas.

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Caro, M.R., Buendía, A.J., Ortega, N. et al. Influence of the Th2 immune response established by Nippostrongylus brasiliensis infection on the protection offered by different vaccines against Chlamydophila abortus infection. Vet Res Commun 29 (Suppl 1), 51–59 (2005). https://doi.org/10.1007/s11259-005-0836-0

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  • DOI: https://doi.org/10.1007/s11259-005-0836-0

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