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Drought and herbivory as modulators of intraspecific differentiation in seedlings of a mountain tree

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Abstract

In mountain ecosystems, plant regeneration might be constrained by multiple factors that change along elevation gradients and promote traits differentiation. Drought is a strong filter for seedling establishment that might be modified by herbivory co-occurrence. Populations of the tree Maytenus boaria support lower soil moisture and higher herbivory pressure at low elevations than at mid-elevations in Córdoba Mountains, central Argentina. Consequently, we expect that populations from the low elevation perform better in response to drought than populations from mid-elevations and that herbivory modifies these responses. Seedlings from the two elevation origins were exposed to two levels of simulated drought and herbivory in a greenhouse experiment. The selected elevations corresponded to the lowest edge of species distribution (with driest soils and highest herbivory pressure) and the central mid-elevation. Performance-related variables, biomass allocation patterns and several morphological and physiological traits were measured. Mortality patterns and most of morphological and physiological variables showed that drought is a stressful factor at the regeneration stage of M. boaria. The drought effect was increased by simulated herbivory in some variables (LMF, RM:SM and SPAD). In most variables, origin did not influence seedling performance, suggesting that drought response of seedlings is independent of populations’ elevation. Only leaf number and water potential were in line with our predictions and showed an origin response to drought.

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Acknowledgements

We are grateful to the staff of Quebrada del Condorito National Park for providing permission for seed collection. We also wish to thank two anonymous reviewers for useful comments on the manuscript and to Jorgelina Brasca who assisted with the English editing.

Funding

This work was supported by CONICET, FONCyT (PICT-2018-04158), SECyT (Universidad Nacional de Córdoba-UNC-), Deuthsche Forschungsgemeinchaft (Grant/ Award Number: HE3041/21-1).

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Authors and Affiliations

  1. Instituto Multidisciplinario de Biología Vegetal, Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET), Universidad Nacional de Córdoba (UNC), Córdoba, Argentina

    P. I. Marcora, S. R. Zeballos, A. E. Ferreras, G. Arias & P. A. Tecco

  2. Facultad de Ciencias Exactas, Físicas y Naturales (FCEFyN), Universidad Nacional de Córdoba, Córdoba, Argentina

    P. A. Tecco

  3. Institute of Biology, Martin-Luther University Halle-Wittenberg, Geobotany & Botanical Garden, Halle (Saale), Germany

    I. Hensen

  4. German Centre for Integrative Biodiversity Research (iDiv) Halle-Jena-Leipzig, Leipzig, Germany

    I. Hensen

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  1. P. I. Marcora
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  2. S. R. Zeballos
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  3. A. E. Ferreras
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  4. G. Arias
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  5. I. Hensen
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Contributions

PM and SZ contributed to study conception and design. Material preparation and data collection were performed by SZ, GA and PM. The analysis data were performed by AF, SZ and PM. The first draft of the manuscript was written by PM and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

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Correspondence to S. R. Zeballos.

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Communicated by Robert Griffin-Nolan.

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Marcora, P.I., Zeballos, S.R., Ferreras, A.E. et al. Drought and herbivory as modulators of intraspecific differentiation in seedlings of a mountain tree. Plant Ecol 224, 895–903 (2023). https://doi.org/10.1007/s11258-023-01345-x

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