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Potential role of glutathione S‑transferase M1 gene polymorphism in kidney calcium oxalate stone formation

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Abstract

Background

The purpose of this study was to look into the effects of glutathione S-transferase M1 (GSTM1) gene polymorphism on the formation of kidney calcium oxalate stones.

Methods

A total of 159 patients with kidney calcium oxalate stones were included in this study as a case group. One hundred and three healthy individuals were included in the control group. The age, gender, and levels of calcium (Ca), uric acid (UA), creatinine (Cr), and urinary creatinine (Ucr) are tracked. Peripheral blood samples are used to perform a polymerase chain reaction to identify the glutathione S-transferase (GST) gene polymorphism (PCR). A commercial kit was used in this study to measure the levels of malondialdehyde (MDA), nitric oxide (NO), total antioxidant capacity (T-AOC), and 8-hydroxydeoxyguanosine (8-OHdG) in peripheral blood.

Results

There was no difference in age or gender distribution between the case and control groups (P > 0.05). The Cr, Ucr, Ca, UA, 8-OHdG, MDA, NO, and T-AOC in the case group were significantly higher than those in the control group (P < 0.001). The Hardy–Weinberg genetic equilibrium test showed no difference between the case group (P = 0.23) and the control group (P = 0.09). In the case group, the 8-OHdG and NO in GSTM1 null genotype were significantly higher than those in GSTM1 genotype (P < 0.05), but there was no significant difference in MDA and T-AOC (P > 0.05). Multivariate regression analysis showed that the GSTM1 null genotype was positively correlated with 8-OHdG (P < 0.001) and NO (P < 0.001).

Conclusions

GSTM1 gene polymorphism might be a detecting risk factor for kidney calcium oxalate stone formation.

Trial registration

ChiCTR2100051300.

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Acknowledgements

The author thanks all the research participants in this study.

Funding

This research was funded by the Guizhou Health Commission’s Science and Technology Fund Project (gzwkj2021-211) and the Doctoral Fund of the Affiliated Hospital of Guiyang Medical College, Guizhou Province, China (C-2012-6).

Author information

Author notes

  1. Kaifa Tang, Shenghan Xu and Pan Chen are contributed equally to this study.

Authors and Affiliations

  1. Department of Urology and Andrology, The First Affiliated Hospital of Guizhou University of Traditional Chinese Medicine, Guiyang, 550001, China

    Kaifa Tang, Shenghan Xu & Bangwei Che

  2. School of Clinical Medicine, Guizhou Medical University, Guiyang, 550004, China

    Kaifa Tang, Pan Chen, Ji Cai, Tao Huang, Miao Liu, Wei Li, Ying Yu & Wenjun Zhang

Authors
  1. Kaifa Tang
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  2. Shenghan Xu
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  3. Pan Chen
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  4. Ji Cai
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  5. Tao Huang
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  6. Miao Liu
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  7. Wei Li
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Contributions

KT conceived and designed the experiments; PC, JC, SX, TH, ML, WL, and YY performed the experiments; PC and SX analyzed the data; SX wrote the paper; and BC and WZ participated in the discussion.

Corresponding author

Correspondence to Kaifa Tang.

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Conflict of interest

There are no conflicts of interest declared by the authors.

Ethical statement

This research involved human participants, and all the participants provided written informed consent. This research was approved by the Ethics Committee of the Affiliated Hospital of Guizhou Medical University, and all work has been carried out in compliance with the Helsinki Declaration.

Research involving human participants and/or animals

The subjects in this study were all humans, and they all gave written informed consent. The Ethics Committee of Guizhou Medical University’s Affiliated Hospital gave its approval to this study (trial registration number: ChiCTR-IPR-14005580). Furthermore, every work was completed in accordance with the Helsinki Declaration.

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Tang, K., Xu, S., Chen, P. et al. Potential role of glutathione S‑transferase M1 gene polymorphism in kidney calcium oxalate stone formation. Int Urol Nephrol 56, 887–892 (2024). https://doi.org/10.1007/s11255-023-03846-0

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  • DOI: https://doi.org/10.1007/s11255-023-03846-0

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