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Prognostic significance of circulating microparticles in IgA nephropathy

  • Nephrology - Original Paper
  • Published:
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Abstract

Purpose

Endothelial injury, involved in the pathogenesis of renal fibrosis, can generate microparticles (MPs). These are 0.1–1 µm membrane-bound vesicles shed from the damaged or activated cell surfaces. We analyzed the presence of circulating MPs and EnMPs in IgAN and correlated with markers of endothelial injury and disease activity.

Methods

The study included 30 IgAN (mean age 31.5 ± 9 years), 25 healthy controls and Lupus nephritis (n = 10) as disease controls. Circulating MPs were quantitated by Flow cytometry and EnMPs were analyzed using anti-CD31-FITC and anti-CD146-PE antibodies. Their levels were correlated with serum von Willebrand Factor, histological Oxford MEST-C score and renal outcome. A prospective validation group of 20 patients of biopsy-proven IgA nephropathy was also included.

Results

IgAN had significantly higher levels of MPs, EnMPs and vWF compared to controls. On multivariate analysis, plasma levels of total MPs, EnMPs and serum vWF correlated significantly with the presence of hypertension and E1 on histology. E1 and high MPs (> 130 counts/µl) were associated with shorter time to doubling of serum creatinine. MPs cutoff level of 130 counts/µl had a sensitivity of 75%, specificity of 93.3% and diagnostic accuracy of 89.5% for E1 in the validation cohort.

Conclusion

Circulating MPs and EnMPs in IgAN correlate with E1 on histology and have a potential as non-invasive biomarkers to predict disease activity and renal outcome.

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Data availability

The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.

Abbreviations

IgAN:

IgA nephropathy

LN:

Lupus nephritis

MPs:

Microparticles

EnMPs:

Endothelial microparticles

PMPs:

Platelet microparticles

vWF:

Von Willebrand factor

FITC:

Fluorescein isothiocyanate

PE:

Phycoerythrin

CKD:

Chronic kidney disease

eGFR:

Estimated glomerular filtration rate

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Acknowledgements

The author is thankful to DST-SERB (CRG/2018/003042) for financial support.

Funding

DST-SERB, CRG/2018/003042, Vinita Agrawal.

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Authors and Affiliations

  1. Departments of Pathology, Sanjay Gandhi Postgraduate Institute of Medical Sciences, Lucknow, 226014, India

    Niharika Bharti, Snigdha Singh, Rakesh Pandey & Vinita Agrawal

  2. Clinical Immunology, Sanjay Gandhi Postgraduate Institute of Medical Sciences, Lucknow, 226014, India

    Mohit Kumar Rai & Vikas Agarwal

  3. Sanjay Gandhi Postgraduate Institute of Medical Sciences, Lucknow, 226014, India

    Narayan Prasad

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  1. Niharika Bharti
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Contributions

NB, VA, RP, VA, and NP participated in study conception and design. NB, and VA, were involved in data acquisition, interpretation and writing the manuscript. MKR helped in flow cytometry experiments. SS helped to collect and evaluate samples for the validation cohort. RP and VA performed critical revision of the manuscript. All the authors read and approved the final manuscript.

Corresponding author

Correspondence to Vinita Agrawal.

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Conflict of interest

There is no conflict of interest.

Ethical approval

The study was approved by the Institutional Ethical Review Committee of Sanjay Gandhi Postgraduate Institute of Medical Sciences in Lucknow, India (IEC code: 2017-129-PhD-99).

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Informed consent was obtained from all participants in the study.

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Bharti, N., Rai, M.K., Singh, S. et al. Prognostic significance of circulating microparticles in IgA nephropathy. Int Urol Nephrol 56, 1071–1081 (2024). https://doi.org/10.1007/s11255-023-03743-6

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  • DOI: https://doi.org/10.1007/s11255-023-03743-6

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