Abstract
Purpose
Recent studies indicate that circulating micro RNAs (miRNAs) are novel class of non-invasive biomarkers with diagnostic and prognostic information. We evaluated the miRNA expressions in bladder cancer (BC) and their associations with disease diagnosis.
Methods
We profiled the expressions of 379 miRNAs in the plasma samples from patients with non-muscle invasive bladder cancer (NMIBC) (n = 34) and non-malignant urological diseases as a control group (n = 32). Patients were evaluated regarding with age, miRNA expressions, by using descriptive statistics. miRNA expression in extracted RNA was quantified using the NanoString nCounter Digital Analyzer.
Results
The analysis of plasma miRNA levels in the marker identification cohort indicated that plasma (miR-1260a, let-7a-3p miR-196b-5p, miR-196a-5p, miR-99a-5p, miR-615-5p, miR-4301, miR-28-3p, miR-4538, miR-1233-3p, miR-4732-5p, miR-1913, miR-1280) levels were increased in NMIBC patients compared to control subjects. There were no significant differences other parameters studied between groups.
Conclusions
The analysis of serum plasma miRNA (miR-1260a, let-7a-3p miR-196b-5p, miR-196a-5p, miR-99a-5p, miR-615-5p, miR-4301, miR-28-3p, miR-4538, miR-1233-3p, miR-4732-5p, miR-1913, miR-1280) levels could be useful plasma biomarkers for BC.
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The study was conducted after Ethics Committee approval of Yuzuncu Yil University Dursun Odabası Medical Center (B.30.2.YYU.0.01.00.00/90) was granted and written informed consent was obtained from patients. The protocol of this study complies with good medical and laboratory practices and recommendations of Helsinki's Declaration of Biomedical Research.
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Kaba, M., Pirinççi, N., Demir, M. et al. The relationship between microRNAs and bladder cancer: are microRNAs useful to predict bladder cancer in suspicious patients?. Int Urol Nephrol 55, 2483–2491 (2023). https://doi.org/10.1007/s11255-023-03666-2
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DOI: https://doi.org/10.1007/s11255-023-03666-2