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Sanjin Paishi Decoction improves the imbalance of gut microbiota and regulates MAPK signaling pathway to inhibit calcium oxalate stones in rats

  • Urology - Original Paper
  • Published:
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Abstract

Introduction

Sanjin Paishi Decoction (SJPSD) has positive effects on stone prevention; however, there is a lack of convincing evidence in the prevention of calcium oxalate stones. This study aimed investigates the effect of SJPSD on calcium oxalate stones and to explore its mechanism.

Methods

The rat model of calcium oxalate stones was established and rats were treated with different doses of SJPSD. The pathological damage of kidney tissues was observed by HE staining, the deposition of calcium oxalate crystals in kidney tissues was examined by Von Kossa staining, and the levels of creatinine (CREA), urea (UREA), calcium (Ca), phosphorus (P), and magnesium (Mg) in serum were analyzed biochemically, the levels of IL-1β, IL-6, and TNF-α in serum were measured by ELISA, and the protein expression of Raf1, MEK1, p-MEK1, ERK1/2, p-ERK1/2, and Cleaved caspase-3 in kidney tissues was analyzed by Western blot. Moreover, the changes in gut microbiota were analyzed by 16S rRNA sequencing.

Results

SJPSD attenuated the pathological damage of renal tissues, reduced the levels of CREA, UREA, Ca, P, and Mg, and inhibited the expression of Raf1, p-MEK1, p-ERK1/2, and Cleaved caspase-3 in renal tissues (P < 0.05). SJPSD treatment affected the composition of intestinal microbiota in rats with calcium oxalate stones.

Conclusion

The mechanism of SJPSD inhibition of calcium oxalate stone injury in rats may be related to the inhibition of the MAPK signaling pathway and regulation of gut microbiota imbalance.

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Data availability

The datasets used and/or analyzed during the current study are available from the corresponding author upon reasonable request.

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Funding

The present study was supported by a research project funded by the Hospital of Chengdu University of Traditional Chinese Medicine (grant no. 20ZL08).

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Author notes

  1. Ying Tian, Juan Zhao and Lan Chen have contributed equally to this work.

Authors and Affiliations

  1. Department of Urology Surgery, Hospital of Chengdu University of Traditional Chinese Medicine, No. 39, Twelve Bridges Road, Jinniu District, Chengdu, 610072, Sichuan Province, China

    Ying Tian, Juan Zhao, Lan Chen, Chuang Zhang & Yuguo Xia

  2. Department of Nursing, Hospital of Chengdu University of Traditional Chinese Medicine, No. 39, Twelve Bridges Road, Jinniu District, Chengdu, 610072, Sichuan Province, China

    Xin Chu

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  1. Ying Tian
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  2. Juan Zhao
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  3. Lan Chen
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  4. Chuang Zhang
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  5. Xin Chu
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  6. Yuguo Xia
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Contributions

YT, XC and YX conceived and designed the research. YT, JZ, LC and CZ performed most of the experiments and wrote the paper. YT and JZ analyzed the data. All authors contributed to the article and approved the submitted version.

Corresponding authors

Correspondence to Xin Chu or Yuguo Xia.

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The authors have no conflicts of interest to declare.

Ethical approval

This study was approved by the Animal Ethics Committee of Chengdu University of Traditional Chinese Medicine (20230331005).

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Tian, Y., Zhao, J., Chen, L. et al. Sanjin Paishi Decoction improves the imbalance of gut microbiota and regulates MAPK signaling pathway to inhibit calcium oxalate stones in rats. Int Urol Nephrol 55, 2421–2429 (2023). https://doi.org/10.1007/s11255-023-03641-x

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  • DOI: https://doi.org/10.1007/s11255-023-03641-x

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