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Correlation between sKL and Nrf2 plasma levels and calcium oxalate urolithiasis

  • Urology - Original Paper
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Abstract

Objective

To investigate the relationship between plasma levels of sKL and Nrf2 and calcium oxalate calculi.

Methods

The clinical data of 135 patients with calcium oxalate calculi treated in the Department of Urology of the second affiliated Hospital of Xinjiang Medical University from February 2019 to December 2022, and 125 healthy persons who underwent physical examination in the same period were collected and divided into healthy group and stone group. The levels of sKL and Nrf2 were measured by ELISA. Correlation test was used to analyze the risk factors of calcium oxalate stones, logistic regression analysis was used to analyze the risk factors of calcium oxalate stones, and ROC curve was used to evaluate the sensitivity and specificity of sKL and Nrf2 in predicting urinary calculi.

Results

Compared with the healthy group, the plasma sKL level in the stone group decreased (111.53 ± 27.89 vs 130.68 ± 32.51), while the plasma Nrf2 level increased (300.74 ± 114.31 vs 246.74 ± 108.22). There was no significant difference in the distribution of age and sex between the healthy group and the stone group, but there were significant differences in plasma levels of WBC, NEUT, CRP, BUN, BUA, SCr, BMI, and eating habits. The results of correlation test showed that the level of plasma Nrf2 was positively correlated with SCr (r = 0.181, P < 0.05) and NEUT (r = 0.144 P < 0.05). Plasma sKL was not significantly correlated with Nrf2 (r = 0.047, P > 0.05), WBC (r = 0.108, P > 0.05), CRP (r = − 0.022, P > 0.05), BUN (r = − 0.115, P > 0.05), BUA (r = − 0.139, P > 0.05), SCr (r = 0.049, P > 0.05), and NEUT (r = 0.027, P > 0.05). Plasma Nrf2 was not significantly correlated with WBC (r = 0.097, P > 0.05), CRP (r = 0.045, P > 0.05), BUN (r = 0.122, P > 0.05), and BUA (r = 0.122, P > 0.05); (r = 0.078, P > 0.05) had no significant correlation. Logistic regression showed that elevated plasma sKL (OR 0.978, 95% CI 0.969 ~ 0.988, P < 0.05) was a protective factor for the occurrence of calcium oxalate stones, BMI (OR 1.122, 95% CI 1.045 ~ 1.206, P < 0.05), dietary habit score (OR 1.571, 95% CI 1.221 ~ 2.020, P < 0.05), and WBC (OR 1.551, 95% CI 1.423 ~ 1.424, P < 0.05). Increased NEUT (OR 1.539, 95% CI 1.391 ~ 1.395, P < 0.05) and CRP (OR 1.118, 95% CI: 1.066 ~ 1.098, P < 0.05) are risk factors for the occurrence of calcium oxalate stones.

Conclusion

Plasma sKL level decreased and Nrf2 level increased in patients with calcium oxalate calculi. Plasma sKL may play an antioxidant role in the pathogenesis of calcium oxalate stones through Nrf2 antioxidant pathway.

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Data availability

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Acknowledgements

This study is supported by the Natural Science Foundation of Xinjiang Uyghur Autonomous region (No. 81760128).

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Authors and Affiliations

  1. Urology Department, Second Affiliated Hospital of Xinjiang Medical University, Urumqi, 830011, Xinjiang, China

    Baihetiyaer Aihemaitijiang & Mulati Mahemuti

  2. Department of Urology, The Second People’s Hospital of Henan Province, Zhengzhou, 450000, Henan Province, China

    Liu Ruotian & Mulati Mahemuti

  3. Department of Surgery, Neijiang Traditional Chinese Medicine Hospital, Neijiang, 641099, Sichuan, China

    Yang Qi & Mulati Mahemuti

Authors
  1. Baihetiyaer Aihemaitijiang
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  2. Liu Ruotian
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  3. Yang Qi
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  4. Mulati Mahemuti
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Contributions

BA: experimental operation and paper writing; MM: data collation and statistical analysis; BA, LR, and MM: research guidance, paper revision, and financial support.

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Correspondence to Mulati Mahemuti.

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Aihemaitijiang, B., Ruotian, L., Qi, Y. et al. Correlation between sKL and Nrf2 plasma levels and calcium oxalate urolithiasis. Int Urol Nephrol 55, 1671–1676 (2023). https://doi.org/10.1007/s11255-023-03615-z

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  • DOI: https://doi.org/10.1007/s11255-023-03615-z

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