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Does intraurethral erythropoietin administration effect wound healing after hypospadias correction? an experimental rat study

  • Urology - Original Paper
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Abstract

Objectives

To analyze effects of intraurethral EPO application on urethral wound healing by defining hystopathologic changes in a rat model of hypospadias.

Methods

A hypospadias model was created in 30 rats and randomized into 3 groups of 10. For 14 days, the first group was administered 25 iu EPO instillation intraurethrally once a day, while group 2 was administered with 50 iu EPO in the same manner. The third group was assigned as control group. On the day 15, rats were sacrificed and penectomies were performed. One independent pathologist who is blinded to groups and treatments evaluated the penis samples.

Results

Histopathologic examinations yielded the mean fibrosis scores (± SD) as 1.9 ± 0.568, 1.1 ± 0.786 and 2.5 ± 0.535 in groups I, II and III, respectively. There was significant difference between the EPO groups and the control group (p = 0.04-I, p = 0.003-II). The mean inflammation scar scores (± SD) were determined as 1 ± 1.054, 2 ± 1.247, 2.63 ± 0.744 in groups I, II and III, respectively. There was a significant difference in terms of inflammation between control group and group I (p = 0.005). Mean congestion scores (± SD) were found 1.2 ± 0.789 in groups I–II and 0.75 ± 0.463 in group III (p = 0.310). Hyperemia was seen in 60% 70% and 37.5% in groups I, II and III, respectively (p = 0.387).

Conclusion

Intraurethral EPO therapy effected urethral wound healing in a good way. Thus it could be feasible to treat the patients with after hypospadias surgeries and to improve success rates.

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Availability of data and material

The datasets generated during and/or analysed during the current study are available from the corresponding author on reasonable request.

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Funding

No funds, grants, or other support was received.

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Authors and Affiliations

  1. Department of Urology, University of Health Sciences, Ankara Training and Research Hospital, Ulucanlar Caddesi No: 89, Ankara, 06230, Turkey

    Eda Tokat, Yildiray Yildiz & Omer Gokhan Doluoglu

  2. Department of Pathology, University of Health Sciences, Ankara Training and Research Hospital, Ankara, Turkey

    Pinar Eylem Eser

  3. Department of Urology, Gazi Yasargil Training and Research Hospital, Diyarbakir, Turkey

    Muhammet Fatih Kilinc

Authors
  1. Eda Tokat
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  2. Yildiray Yildiz
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  3. Pinar Eylem Eser
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  4. Muhammet Fatih Kilinc
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  5. Omer Gokhan Doluoglu
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Contributions

E Tokat: protocol/project development, data collection or management, data analysis, manuscript writing/editing. Y Yıldız: protocol/project development, data collection or management. EP Eser: data collection or management, data analysis. MF Kılınç: protocol/project development, data collection or management, manuscript writing/editing. ÖG Doluoğlu: manuscript writing/editing.

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Correspondence to Eda Tokat.

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The authors declare no conflicts of interest that are relevant to the content of this article.

Ethics approval

The study was approved by the Local Ethics Committee of Ankara Training and Research Hospital (Reference number: 63-642).

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Tokat, E., Yildiz, Y., Eser, P.E. et al. Does intraurethral erythropoietin administration effect wound healing after hypospadias correction? an experimental rat study. Int Urol Nephrol 53, 2057–2062 (2021). https://doi.org/10.1007/s11255-021-02912-9

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  • DOI: https://doi.org/10.1007/s11255-021-02912-9

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