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Potential protective effect of leptin and uncoupling protein-2 genes polymorphism in Egyptian patients with chronic kidney disease

  • Nephrology - Original Paper
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Abstract

Background

Kidney disease is a serious public health problem worldwide. It is the fifth top-ranking cause of death in Egypt, causing approximately 3.98% of all deaths. This study’s objective was to examine whether an association exists between leptin (− 2548G/A) and uncoupling protein-2 45 bp I/D genes, individually and collectively, in CKD and progression to ESRD.

Methods

One hundred patients (69 males, 31 females) aged (47.1 ± 16.11 years) with ESRD, 40 patients (19 males, 21 females) aged (43.15 ± 10.00 years with CKD, and 50 healthy controls (23 males, 27 females) aged (37.84 ± 1.95 years) were enrolled. Polymerase chain reaction (PCR) was employed to measure variation in gene expression among the study groups. The frequency of single nucleotide polymorphisms (SNP) genotypes were identified in controls, CKD and ESRD patients.

Results

Leptin genotypes were associated with lower CKD incidence in control versus study subjects (95% CI = (0.08–0.63), P = 0.01) with risk value equal to 0.22 < 1, G/A genotype was significantly lower in CKD than ESRD groups. There was no correlation between UCP-2 I/D genotype and CKD (P = 0.27). There was no correlation between the UCP-2 gene and the progression to ESRD.

Conclusions

This study suggests that, Leptin − 2548G/A gene may be a promising marker for early detection of ESRD in Egyptian patients. G/A genotype might inhibit the development of CKD to ESRD.

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Funding

This study was not supported by any dedicated research funding.

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Authors and Affiliations

  1. Biochemistry Department, Faculty of Science, Ain Shams University, Cairo, Egypt

    Esraa A. Ghazaly & Dina M. Seoudi

  2. Genetic Unit, Children Hospital, Mansoura University, Mansoura, Egypt

    Afaf M. EL-Saeed

  3. Mansoura Nephrology and Dialysis Unit, Faculty of Medicine, Mansoura University, Mansoura, Egypt

    Mostafa Abdelsalam

Authors
  1. Esraa A. Ghazaly
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  2. Afaf M. EL-Saeed
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  3. Mostafa Abdelsalam
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  4. Dina M. Seoudi
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Contributions

EG1: study concept, drafting, design, statistical analysis, and approval of the manuscript. DS2: study concept, drafting, design, and approval of the manuscript. AE3: study concepting, drafting, design, and approval of the manuscript. MA: data collection and approval of the manuscript.

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Correspondence to Esraa A. Ghazaly.

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Authors have nothing to disclose.

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Voluntary informed consent was given by all participants. Privacy and confidentiality of all participants' personal, clinical, and laboratory data were maintained throughout the study. This work was carried out in accordance with the rules of the Declaration of Helsinki

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Ghazaly, E.A., EL-Saeed, A.M., Abdelsalam, M. et al. Potential protective effect of leptin and uncoupling protein-2 genes polymorphism in Egyptian patients with chronic kidney disease. Int Urol Nephrol 52, 2153–2160 (2020). https://doi.org/10.1007/s11255-020-02567-y

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  • DOI: https://doi.org/10.1007/s11255-020-02567-y

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