Skip to main content
SpringerLink
Log in

Lymphocyte and monocyte vitamin D receptor expression during paricalcitol or calcitriol treatments in patients with stage 5 chronic kidney disease

  • Nephrology - Original Paper
  • Published:
International Urology and Nephrology Aims and scope Submit manuscript

Abstract

Purpose

In this study, we aimed to investigate the effect of paricalcitol and calcitriol usage on vitamin D receptor (VDR) contents of CD8+ , CD4+ lymphocytes and monocytes in stage 5d chronic kidney disease (CKD) patients.

Methods

Thirty-six hemodialysis patients older than 18 years of age and 19 healthy controls (group HC) without any known acute or chronic diseases were included in the study. The group of patients undergoing scheduled hemodialysis comprised three subgroups: group CL: patients on calcitriol (n: 10), group PC: patients on paricalcitol (n: 13), and group NT: patients not taking any vitamin D or VDR activating medications (n: 13). CD8+/VDR, CD4+/VDR and MONO/VDR values were representing the ratio of VDR representing cells among related cell group. On the other hand, values of CD8+/MFI, CD4+/MFI and MONO/MFI have shown the total amount of cellular VDR content per cell which has been given as of mean fluorescence intensity in the flow cytometric process. Main CKD mineral bone disorder parameters such as a hemogram, serum BUN, creatinine, albumin, Ca, iP, iPTH, 25(OH)D3 levels were also measured.

Results

Average VDR contents in CD8+, CD4+ and monocytes were not different among three patient groups on hemodialysis. But in all hemodialysis subgroups, CD8+/VDR, CD4+/VDR, MONO/VDR, CD8+/MFI, CD4+/MFI and MONO/MFI levels were found to be higher compared with the healthy control subjects (p < 0.001). Among hemodialysis groups, no significant CD8+/VDR, CD4+/VDR, and MONO/VDR content differences were found with regard to the type of VDR activator agent used. There was no difference in serum levels of 25(OH)D3 and CRP among groups participating in the study.

Conclusion

There was no difference between CD8+/VDR, CD4+/VDR, and MONO/VDR levels in hemodialysis patients using calcitriol or paricalcitol, suggesting that both treatment agents may have a similar effect on VDR contents in lymphocytes and monocytes in that patient population. But in all hemodialysis subgroups, CD8+/VDR, CD4+/VDR, and MONO/VDR levels were found to be higher compared with the healthy control subjects, suggesting an overexpression of VDR through a non CRP and/or 25(OH)D3 dependent mechanism.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Bolton CH, Downs LG, Victory JG, Dwight JF, Tomson CR, Mackness MI et al (2001) Endothelial dysfunction in chronic renal failure: roles of lipoprotein oxidation and pro-inflammatory cytokines. Nephrol Dial Transplant 16(6):1189–1197

    Article  CAS  Google Scholar 

  2. Sarnak MJ, Levey AS (2000) Cardiovascular disease and chronic renal disease: a new paradigm. Am J Kidney Dis 35(4 Suppl 1):117–131

    Article  Google Scholar 

  3. Stenvinkel P (2002) Inflammation in end-stage renal failure: could it be treated? Nephrol Dial Transplant 17(Suppl 8):33–38

    Article  Google Scholar 

  4. Akchurin OM, Kaskel F (2015) Update on inflammation in chronic kidney disease. Blood Purif 39(1–3):84–92

    Article  CAS  Google Scholar 

  5. Holick MF, Chen TC (1080S) Vitamin D deficiency: a worldwide problem with health consequences. Am J Clin Nutr 87(4):1080S–S1086

    Article  CAS  Google Scholar 

  6. van Etten E, Mathieu C (2005) Immunoregulation by 1, 25-dihydroxyvitamin D3: basic concepts. J Steroid Biochem Mol Biol 97(1–2):93–101

    Article  Google Scholar 

  7. Maalouf NM (2008) The non-calciotropic actions of vitamin D: recent clinical developments. Curr Opin Nephrol Hypertens 17(4):408–415

    Article  CAS  Google Scholar 

  8. Hochberg ZE (2003) Vitamin D and rickets. Karger Medical and Scientific Publishers, Basel (e-ISBN: 978-3-318-00975-0)

    Book  Google Scholar 

  9. Liu C, Li H (2019) Correlation of the severity of chronic kidney disease with serum inflammation, osteoporosis and vitamin D deficiency. Exp Ther Med 17(1):368–372

    CAS  PubMed  Google Scholar 

  10. Lopez-Munoz P, Beltran B, Saez-Gonzalez E, Alba A, Nos P, Iborra M (2019) Influence of vitamin D deficiency on inflammatory markers and clinical disease activity in IBD patients. Nutrients. 11:5

    Article  Google Scholar 

  11. Lucas RM, Gorman S, Geldenhuys S, Hart PH (2014) Vitamin D and immunity. F1000Prime Rep 6:118

    Article  Google Scholar 

  12. Özkan B, Döneray H (2011) D vitamininin iskelet sistemi dişi etkileri. Cocuk Sagligi Hastaliklari Dergisi 54(2):99–119

    Google Scholar 

  13. Haussler MR, Whitfield GK, Haussler CA, Hsieh JC, Thompson PD, Selznick SH et al (1998) The nuclear vitamin D receptor: biological and molecular regulatory properties revealed. J Bone Miner Res 13(3):325–349

    Article  CAS  Google Scholar 

  14. Zhang Y, Leung DY, Richers BN, Liu Y, Remigio LK, Riches DW et al (2012) Vitamin D inhibits monocyte/macrophage proinflammatory cytokine production by targeting MAPK phosphatase-1. J Immunol. 188(5):2127–2135

    Article  CAS  Google Scholar 

  15. Baeke F, Takiishi T, Korf H, Gysemans C, Mathieu C (2010) Vitamin D: modulator of the immune system. Curr Opin Pharmacol 10(4):482–496

    Article  CAS  Google Scholar 

  16. Li YC, Kong J, Wei M, Chen ZF, Liu SQ, Cao LP (2002) 1, 25-Dihydroxyvitamin D 3 is a negative endocrine regulator of the renin-angiotensin system. J Clin Invest 110(2):229–238

    Article  CAS  Google Scholar 

  17. Vaziri ND (2004) Roles of oxidative stress and antioxidant therapy in chronic kidney disease and hypertension. Curr Opin Nephrol Hypertens 13(1):93–99

    Article  CAS  Google Scholar 

  18. Welsh J (2012) Cellular and molecular effects of vitamin D on carcinogenesis. Arch Biochem Biophys 523(1):107–114

    Article  CAS  Google Scholar 

  19. Sugden JA, Davies JI, Witham MD, Morris AD, Struthers AD (2008) Vitamin D improves endothelial function in patients with Type 2 diabetes mellitus and low vitamin D levels. Diabet Med 25(3):320–325

    Article  CAS  Google Scholar 

  20. Sanz AB, Izquierdo MC, Sanchez-Nino MD, Ucero AC, Egido J, Ruiz-Ortega M et al (2014) TWEAK and the progression of renal disease: clinical translation. Nephrol Dial Transplant 29(Suppl 1):i54–i62

    Article  CAS  Google Scholar 

  21. Gupta J, Mitra N, Kanetsky PA, Devaney J, Wing MR, Reilly M et al (2012) Association between albuminuria, kidney function, and inflammatory biomarker profile in CKD in CRIC. Clin J Am Soc Nephrol 7(12):1938–1946

    Article  CAS  Google Scholar 

  22. Almeida ACSF, Siqueira MC, Bonan NB, Dambiski A, Bertuzzo G, Moreno-Amaral AN et al (2019) Vitamin D levels reverberate in monocytes modulation in hemodialysis patients. J Cell Physiol. 2019:25. https://doi.org/10.1002/jcp.28290

    Article  CAS  Google Scholar 

  23. Ateş I, Ozkayar N, Yılmaz FM, Bayrakçı N, Neselioğlu S, Erel O et al (2018) Oxidative stress level in patients with chronic kidney disease. Ortadogu Med J 10(1):45–50

    Google Scholar 

  24. Levin A, Bakris GL, Molitch M, Smulders M, Tian J, Williams LA et al (2007) Prevalence of abnormal serum vitamin D, PTH, calcium, and phosphorus in patients with chronic kidney disease: results of the study to evaluate early kidney disease. Kidney Int 71(1):31–38

    Article  CAS  Google Scholar 

  25. Graeff-Armas LA, Kaufmann M, Lyden E, Jones G (2018) Serum 24, 25-dihydroxyvitamin D3 response to native vitamin D2 and D3 Supplementation in patients with chronic kidney disease on hemodialysis. Clin Nutr 37(3):1041–1045

    Article  CAS  Google Scholar 

  26. Ogunkolade BW, Boucher BJ, Prahl JM, Bustin SA, Burrin JM, Noonan K et al (2002) Vitamin D receptor (VDR) mRNA and VDR protein levels in relation to vitamin D status, insulin secretory capacity, and VDR genotype in Bangladeshi Asians. Diabetes 51(7):2294–2300

    Article  CAS  Google Scholar 

  27. Peacock M (2010) Calcium metabolism in health and disease. Clin J Am Soc Nephrol 5(Suppl 1):23–30

    Article  Google Scholar 

  28. Çidem M, Karacan İ, Beytemur O, Kara S (2017) Prevalence and risk factors for vitamin D deficiency in patients with widespread musculoskeletal pain. Turk J Med Sci. 47(3):728–731

    Article  Google Scholar 

Download references

Funding

No financial disclosure was declared by the authors.

Author information

Authors and Affiliations

  1. Department of Internal Medicine, Akdeniz University Medical School, Antalya, Turkey

    Hasan Sözel

  2. Department of Microbiology, Division of Immunology, Akdeniz University Medical School, Antalya, Turkey

    Sadi Köksoy

  3. Department of Biochemistry, Akdeniz University Medical School, Antalya, Turkey

    Sebahat Ozdem

  4. Department of Nephrology, Antalya Atatürk State Hospital, Üçgen Mah. Güllük Cad. No:100 Muratpaşa, Antalya, Turkey

    Fatih Yılmaz

  5. Department of Internal Medicine, Division of Nephrology, Akdeniz University Medical School, Antalya, Turkey

    Feyza Bora & F. Fevzi Ersoy

Authors
  1. Hasan Sözel
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Sadi Köksoy
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Sebahat Ozdem
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Fatih Yılmaz
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Feyza Bora
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. F. Fevzi Ersoy
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Fatih Yılmaz.

Ethics declarations

Conflict of interest

No conflict of interest was declared by the authors.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Sözel, H., Köksoy, S., Ozdem, S. et al. Lymphocyte and monocyte vitamin D receptor expression during paricalcitol or calcitriol treatments in patients with stage 5 chronic kidney disease. Int Urol Nephrol 52, 1563–1570 (2020). https://doi.org/10.1007/s11255-020-02475-1

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11255-020-02475-1

Keywords

Search

Navigation