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The clinical value of the prostatic exosomal protein expression in the diagnosis of chronic prostatitis: a single-center study

  • Urology - Original Paper
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Abstract

Objective

Levels of urinary prostatic exosomal protein (PSEP) were detected to evaluate the clinical potential of PSEP as a diagnostic marker of chronic prostatitis (CP).

Materials and methods

The level of urinary PSEP was measured in 412 cases by an enzyme-linked immunosorbent assay kit, including 202 controls and 210 CP cases. Of the CP patients, 116 cases met the definition of the USA National Institutes of Health category III (NIH-III), with 60 cases of NIH-IIIA and 56 cases of NIH-IIIB. The ages, body mass indexes (BMI), white blood cell (WBC) levels in expressed prostatic secretions (EPS), lecithin body counts in EPS, urine PSEP levels both before and after prostate massage obtained from the CP patients and NIH-CPSI scores were analyzed.

Results

In the diagnosis of CP, the PSEP contents in the urine samples before and after prostate massage manifested a sensitivity of 86.93% vs. 61.06%, and a total coincidence rate of 85.24% vs. 61.06%, respectively. The area under the ROC curve was 0.926 vs. 0.709 for the before and after massage PSEP contents, respectively. Besides, during the follow-up of patients with CP, the improvement in symptoms was not correlated with the level changes of PSEP.

Conclusion

Measurement of PSEP levels for the clinical diagnosis of CP is objective and painless. It could be a novel, simple, and noninvasive method for the diagnosis of CP. However, differences in fluid intake may result in a concentration or dilution of urine, which would ultimately affect the judgment of PSEP results.

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References

  1. Collins MM, Stafford RS, O’Leary MP, Barry MJ (1998) How common is prostatitis? A national survey of physician visits. J Urol 159(4):1224–1228

    Article  CAS  Google Scholar 

  2. Clemens JQ, Meenan RT, O’Keeffe Rosetti MC, Kimes T, Calhoun EA (2007) Prevalence of and risk factors for prostatitis: population based assessment using physician assigned diagnoses. J Urol 178(4 Pt 1):1333–1337. https://doi.org/10.1016/j.juro.2007.05.140

    Article  PubMed  Google Scholar 

  3. Wang J, Yan D, Liang K, Xu Z (2016) A randomized controlled trial of levofloxacin, terazosin, and combination therapy in patients with category III chronic prostatitis/chronic pelvic pain syndrome. Int Urol Nephrol 48(1):13–18. https://doi.org/10.1007/s11255-015-1147-1

    Article  CAS  PubMed  Google Scholar 

  4. McNaughton CM, Pontari MA, O’Leary MP, Calhoun EA, Santanna J, Landis JR, Kusek JW, Litwin MS, Chronic Prostatitis Collaborative Research N (2001) Quality of life is impaired in men with chronic prostatitis: the Chronic Prostatitis Collaborative Research Network. Journal of general internal medicine 16(10):656–662. https://doi.org/10.1111/j.1525-1497.2001.01223.x

    Article  Google Scholar 

  5. Nickel JC, Nyberg LM, Hennenfent M (1999) Research guidelines for chronic prostatitis: consensus report from the first National Institutes of Health International Prostatitis Collaborative Network. Urology 54(2):229–233

    Article  CAS  Google Scholar 

  6. Mo MQ, Long LL, Xie WL, Chen S, Zhang WH, Luo CQ, Deng LW (2014) Sexual dysfunctions and psychological disorders associated with type IIIa chronic prostatitis: a clinical survey in China. Int Urol Nephrol 46(12):2255–2261. https://doi.org/10.1007/s11255-014-0810-2

    Article  PubMed  Google Scholar 

  7. Polackwich AS, Shoskes DA (2016) Chronic prostatitis/chronic pelvic pain syndrome: a review of evaluation and therapy. Prostate Cancer Prostatic Dis 19(2):132–138. https://doi.org/10.1038/pcan.2016.8

    Article  CAS  PubMed  Google Scholar 

  8. Aalberts M, Stout TA, Stoorvogel W (2014) Prostasomes: extracellular vesicles from the prostate. Reproduction 147(1):R1–14. https://doi.org/10.1530/REP-13-0358

    Article  CAS  PubMed  Google Scholar 

  9. Bjartell A, Montironi R, Berney DM, Egevad L (2011) Tumour markers in prostate cancer II: diagnostic and prognostic cellular biomarkers. Acta Oncol 50(Suppl 1):76–84. https://doi.org/10.3109/0284186X.2010.531284

    Article  PubMed  Google Scholar 

  10. Graddis TJ, McMahan CJ, Tamman J, Page KJ, Trager JB (2011) Prostatic acid phosphatase expression in human tissues. Int J Clin Exp Pathol 4(3):295–306

    PubMed  PubMed Central  Google Scholar 

  11. Cho SY, Choi K, Jeon JH, Kim CW, Shin DM, Lee JB, Lee SE, Kim CS, Park JS, Jeong EM, Jang GY, Song KY, Kim IG (2010) Differential alternative splicing of human transglutaminase 4 in benign prostate hyperplasia and prostate cancer. Exp Mol Med 42(4):310–318. https://doi.org/10.3858/emm.2010.42.4.031

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  12. Zeng Y, Zhang J, Chen Y-H, Onco Biomedical Technology Suzhou CO. L (2015) Establishment of ELISA detection method for prostate exosomal protein and its primary evaluation. Prac J med Pham 32(10):885–888

    Google Scholar 

  13. Nopparat J, Zhang J, Lu JP, Chen YH, Zheng D, Neufer PD, Fan JM, Hong H, Boykin C, Lu Q (2015) delta-Catenin, a Wnt/beta-catenin modulator, reveals inducible mutagenesis promoting cancer cell survival adaptation and metabolic reprogramming. Oncogene 34(12):1542–1552. https://doi.org/10.1038/onc.2014.89

    Article  CAS  PubMed  Google Scholar 

  14. Pontari MA, Ruggieri MR (2008) Mechanisms in prostatitis/chronic pelvic pain syndrome. The J Urol 179(5 Suppl):S61–S67. https://doi.org/10.1016/j.juro.2008.03.139

    Article  PubMed  Google Scholar 

  15. Lotti F, Corona G, Mondaini N, Maseroli E, Rossi M, Filimberti E, Noci I, Forti G, Maggi M (2014) Seminal, clinical and colour-Doppler ultrasound correlations of prostatitis-like symptoms in males of infertile couples. Andrology 2(1):30–41. https://doi.org/10.1111/j.2047-2927.2013.00156.x

    Article  CAS  PubMed  Google Scholar 

  16. Nickel JC (2003) Recommendations for the evaluation of patients with prostatitis. World J Urol 21(2):75–81. https://doi.org/10.1007/s00345-003-0328-1

    Article  PubMed  Google Scholar 

  17. Pons-Rejraji H, Artonne C, Sion B, Brugnon F, Canis M, Janny L, Grizard G (2011) Prostasomes: inhibitors of capacitation and modulators of cellular signalling in human sperm. Int J Androl 34(6 Pt 1):568–580. https://doi.org/10.1111/j.1365-2605.2010.01116.x

    Article  CAS  PubMed  Google Scholar 

  18. Stewart AB, Anderson W, Delves G, Lwaleed BA, Birch B, Cooper A (2004) Prostasomes: a role in prostatic disease? BJU Int 94(7):985–989. https://doi.org/10.1111/j.1464-410X.2004.05091.x

    Article  PubMed  Google Scholar 

  19. Minelli A, Ronquist G, Carlsson L, Mearini E, Nilsson O, Larsson A (2005) Antiprostasome antibody titres in benign and malignant prostate disease. Anticancer Res 25(6C):4399–4402

    PubMed  Google Scholar 

  20. Utleg AG, Yi EC, Xie T, Shannon P, White JT, Goodlett DR, Hood L, Lin B (2003) Proteomic analysis of human prostasomes. Prostate 56(2):150–161. https://doi.org/10.1002/pros.10255

    Article  CAS  PubMed  Google Scholar 

  21. Yin L, Tang Y, Pan A, Yang L, Zhu X, Liu Y (2019) The application of IL-10 and TNF-alpha in expressed prostatic secretions and prostatic exosomal protein in urine in the diagnosis of patients with chronic prostatitis. Medicine 98(33):e16848. https://doi.org/10.1097/MD.0000000000016848

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  22. Watanabe T, Inoue M, Sasaki K, Araki M, Uehara S, Monden K, Saika T, Nasu Y, Kumon H, Chancellor MB (2011) Nerve growth factor level in the prostatic fluid of patients with chronic prostatitis/chronic pelvic pain syndrome is correlated with symptom severity and response to treatment. BJU Int 108(2):248–251. https://doi.org/10.1111/j.1464-410X.2010.09716.x

    Article  CAS  PubMed  Google Scholar 

  23. Wei X, Zhang G, Yuan H, Ding X, Li S, Zhang X, Hou J (2011) Detection and quantitation of soluble B7-H3 in expressed prostatic secretions: a novel marker in patients with chronic prostatitis. J Urol 185(2):532–537. https://doi.org/10.1016/j.juro.2010.09.104

    Article  CAS  PubMed  Google Scholar 

  24. Soric T, Selimovic M, Bakovic L, Simurina T, Selthofer R, Dumic J (2017) Clinical and biochemical influence of prostatic stones. Urol Int 98(4):449–455. https://doi.org/10.1159/000455161

    Article  CAS  PubMed  Google Scholar 

  25. Li X, Jiang T, Liu F, Shao X, Xu Y, Sheng W, Sun W (2018) Clinical evaluation of urine prostatic exosomal protein in the diagnosis of chronic prostatitis. Urol Int 100(1):112–118. https://doi.org/10.1159/000479188

    Article  CAS  PubMed  Google Scholar 

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Acknowledgements

The presented research was financially supported by the National Science Foundation for Young Scientists (81400757 and 81802827), National Natural Science Foundation of China (31430028, 81630019 and 81870519), Scientific Research Foundation of the Institute for Translational Medicine of Anhui Province (2017ZHYX02), the Natural Science Foundation of Anhui Province, China (KJ2019A0277) and the Natural Science Foundation of Guangdong Province, China (2017A030313800).

Author information

Author notes

  1. Xingliang Feng and Meng Zhang contributed equally to the work.

Authors and Affiliations

  1. Department of Urology, The First Affiliated Hospital of Anhui Medical University and Institute of Urology and Anhui Province Key Laboratory of Genitourinary Diseases, Anhui Medical University, Jixi Road, Shushan District, Hefei, Anhui, 230022, People’s Republic of China

    Xingliang Feng, Meng Zhang, Ligang Zhang, Li Zhang, Xiansheng Zhang, Song Fan & Chaozhao Liang

  2. Urology Institute of Shenzhen University, The Third Affiliated Hospital of Shenzhen University, Shenzhen University, Shenzhen, 518000, People’s Republic of China

    Meng Zhang

  3. Department of Health Examination Center, The First Affiliated Hospital of Anhui Medical University, Anhui Medical University, Hefei, 230022, PR China

    Huaqing Hu

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  1. Xingliang Feng
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Contributions

CL, SF, LZ and XZ designed this experiment. XF, LZ and MZ performed the research and analyzed the data. XF and MZ wrote the manuscript, and then HH, SF, LZ and CL revised the manuscript. All authors read and approved the final manuscript.

Corresponding authors

Correspondence to Song Fan or Chaozhao Liang.

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The research contents and research programs were reviewed and approved by the Ethics Committee of the First Affiliated Hospital of Anhui Medical University. (anyiyifuyuanlunshen-kuai-PJ-2019-09-11).

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Feng, X., Zhang, M., Zhang, L. et al. The clinical value of the prostatic exosomal protein expression in the diagnosis of chronic prostatitis: a single-center study. Int Urol Nephrol 52, 225–232 (2020). https://doi.org/10.1007/s11255-019-02313-z

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