Is it possible to prevent contrast-induced nephropathy with dexpanthenol?

  • Osman Sutcuoglu
  • Mehmet Kursat DericiEmail author
  • Ozge Tugce Pasaoglu
  • Burak Dumludag
  • Ozant Helvacı
  • Betul Ogut
  • Ipek Isık Gonul
  • Ulver Derici
Nephrology - Original Paper



Contrast-induced nephropathy (CIN) is one of the side effects of diagnostic procedures. Oxidative stress plays an important role in CIN’s pathophysiology. Dexpanthenol (Dexp) is a substance with antioxidant efficacy. We investigated the likely protective effects of dexpanthenol for CIN.


Twenty-four Sprague–Dawley rats were divided randomly into four groups of 6 rats; control (group 1), Dexp (group 2), CIN (group 3) and Dexp + CIN (group 4). All rats were restricted of water moderately to facilitate of nephrotoxicity. Dexp was administered into the intraperitoneally at a dose of 500 mg/kg for 5 days in groups 2 and 4. The same amount of saline was applied via intraperitoneally to group 1 and 3. In CIN and Dexp + CIN groups, L-NAME (10 mg/kg), tenoxicam (0.5 mg/kg) and sodium amidotrizoate (10 ml/kg) were administered on the 4th day via the tail vein for CIN. All rats were euthanized on the 6th day and samples for biochemical and pathological evaluations were collected.


When the Dexp + CIN group and the CIN group were compared, it was found to be provide a significant decline at the level of acute tubular injury and necrosis in kidney biopsies by dexp. Furthermore Dexp significantly reduced the serum cystatin C (Cys-C) levels, not serum creatinine. There was no statistically significant difference between the groups in total oxidant and antioxidant levels.


Dexpanthenol did not have significant effect on oxidative stress of acute kidney injury on this rat model. However, it has ameliorated serum Cys-C levels and histopathological findings of CIN.


Radiocontrast media Nephrotoxicity Dexpanthenol Cystatin C Apoptosis 



This study was supported by Turkish Society of Hypertension and Kidney Disease.

Compliance with ethical standards

Conflict of interest

All authors have no conflict of interest to declare.

Ethical approval

Experiments were performed in accordance with the criteria about the care and use of Laboratory Animals of Gazi University Local Ethics Committee.


  1. 1.
    Mohammed NM, Mahfouz A, Achkar K, Rafie IM, Hajar R (2013) Contrast-induced nephropathy. Heart Views 14:106–116CrossRefGoogle Scholar
  2. 2.
    Finn WF (2006) The clinical and renal consequences of contrast-induced nephropathy. Nephrol Dial Transplant 21(Suppl 1):i2–i10. CrossRefGoogle Scholar
  3. 3.
    Gleeson TG, Bulugahapitiya S (2004) Contrast-induced nephropathy. AJR Am J Roentgenol 183:1673–1689CrossRefGoogle Scholar
  4. 4.
    Calvin AD, Misra S, Pflueger A (2010) Contrast-induced acute kidney injury and diabetic nephropathy. Nat Rev Nephrol 6:679–688CrossRefGoogle Scholar
  5. 5.
    Wong PC, Li Z, Guo J, Zhang A (2012) Pathophysiology of contrast-induced nephropathy. Inter J Cardiol 158:186–192CrossRefGoogle Scholar
  6. 6.
    Azzalini L, Spagnoli V, Ly HQ (2016) Contrast-induced nephropathy: from pathophysiology to preventive strategies. Can J Cardiol 32:247–255CrossRefGoogle Scholar
  7. 7.
    Altintas R, Parlakpinar H, Beytur A, Vardi N, Polat A, Sagir M, Odabas GP (2012) Protective effect of dexpanthenol on ischemia-reperfusion-induced renal injury in rats. Kidney Blood Press Res 36(1):220–230. (Epub 2012 Nov 15) CrossRefGoogle Scholar
  8. 8.
    Sen H, Deniz S, Yedekci AE, Inangil G, Muftuoglu T, Haholu A, Ozkan S (2014) Effects of dexpanthenol and N-acetylcysteine pretreatment in rats before renal ischemia/reperfusion injury. Ren Fail 36:1570–1574CrossRefGoogle Scholar
  9. 9.
    Kumerova AO, Utno LI, Lipsberga ZE, Shkestere II (1992) Study of pantothenic acid derivatives as cardiac protectors in a model of experimental ischemia and reperfusion of the isolated heart. Biull Eksp Biol Med 113:373–375CrossRefGoogle Scholar
  10. 10.
    Soylu Karapinar O, Pinar N, Özcan O, Atik Doğan E, Bayraktar S, Şahin H, Dolapçioğlu K (2017) The effect of dexpanthenol on experimentally induced ovarian ischaemia/reperfusion injury: a biochemical and histopathological evaluation. Arch Gynecol Obstet 295:777–784CrossRefGoogle Scholar
  11. 11.
    Zakaria MM, Hajipour B, Khodadadi A, Afshari F (2011) Ameliorating effects of dexpanthenol in cerebral ischaemia reperfusion induced injury in rat brain. J Pak Med Assoc 61:889–892Google Scholar
  12. 12.
    Gupta RK, Bang TJ (2010) Prevention of contrast-induced nephropathy (CIN) in interventional radiology practice. Semin Intervent Radiol 27:348–359CrossRefGoogle Scholar
  13. 13.
    Lameire N, Kellum JA (2013) KDIGO AKI Guideline Work. Group contrast-induced acute kidney injury and renal support for acute kidney injury: a KDIGO summary (Part 2). Crit Care 17(1):205. CrossRefGoogle Scholar
  14. 14.
    Powell SB, Swerdlow NR, Pitcher LK, Geyer MA (2002) Isolation rearing-induced deficits in prepulse inhibition and locomotor habituation are not potentiated by water deprivation. Physiol Behav 77:55–64CrossRefGoogle Scholar
  15. 15.
    Singh AP, Junemann A, Muthuraman A, Jaggi AS, Singh N, Grover K, Dhawan R (2012) Animal models of acute renal failure. Pharmacol Rep 64:31–44CrossRefGoogle Scholar
  16. 16.
    Agmon Y, Peleg H, Greenfeld Z, Rosen S, Brezis M (1994) Nitric oxide and prostanoids protect the renal outer medulla from radiocontrast toxicity in rats. J Clin Invest 94:1069–1075CrossRefGoogle Scholar
  17. 17.
    Hunter MI, Nlemadim BC, Davidson DL (1985) Lipid peroxidation products and antioxidant proteins in plasma and cerebrospinal fluids from multiple sclerosis patients. Neurochem Res 10:1645–1652CrossRefGoogle Scholar
  18. 18.
    Thomsen HS, Morcos SK (2003) Contrast media and the kidney: European Society of Urogenital Radiology (ESUR) guidelines. Br J Radiol 76:513–518CrossRefGoogle Scholar
  19. 19.
    Morcos SK, Thomsen HS, Webb JAW (1999) Contrast-media-induced nephrotoxicity: a consensus report. Eur Radiol 9:1602–1613CrossRefGoogle Scholar
  20. 20.
    Zhao Q, Yin J, Lu Z, Kong Y, Zhang G, Zhao B, Wang F (2016) Sulodexide protects contrast-induced nephropathy in Sprague–Dawley rats. Cell Physiol Biochem 40:621–632CrossRefGoogle Scholar
  21. 21.
    Matsunami T, Hino K, Dosho R, Miyatake S, Ebisu G, Kuwatsuru R (2017) Efficacy of oral supplemental hydration for the prevention of contrast induced nephropathy in rats. Jpn J Radiol 35:190–196CrossRefGoogle Scholar
  22. 22.
    Herget-Rosenthal S, Marggraf G, Hüsing J, Göring F, Pietruck F, Janssen O, Philipp T, Kribben A (2004) Early detection of acute renal failure by serum cystatin C. Kidney Int 66:1115–1122CrossRefGoogle Scholar
  23. 23.
    Geenen RWF, Kingma HJ, Van der Molen AJ (2013) Contrast-induced nephropathy: pharmacology, pathophysiology and prevention. Insights Imaging. 4:811–820CrossRefGoogle Scholar
  24. 24.
    Malis CD, Bonventre JV (1988) Susceptibility of mitochondrial membranes to calcium and reactive oxygen species: implications for ischemic and toxic tissue damage. Prog Clin Biol Res 282:235–259Google Scholar
  25. 25.
    Malek M, Nematbakhsh M (2015) Renal ischemia/reperfusion injury; from pathophysiology to treatment. J Renal Inj Prev. 4:20–27Google Scholar
  26. 26.
    Yang SK, Duan SB, Pan P, Xu XQ, Liu N, Xu J (2015) Preventive effect of pentoxifylline on contrast–induced acute kidney injury in hypercholesterolemic rats. Exper Ther Med 9:384–388CrossRefGoogle Scholar
  27. 27.
    Doğan EE, Erkoç R, Ekinci I, Hamdard J, Döner B, Çıkrıkçıoğlu MA, Karatoprak C, Çoban G, Özer ÖF, Kazancıoğlu R (2017) Protective effect of dexpanthenol against nephrotoxic effect of amikacin: an experimental study. Biomed Pharmacother 89:1409–1414CrossRefGoogle Scholar
  28. 28.
    Gao D, Wang H, Xu Y, Zheng D, Zhang Q, Li W (2018) Protective effect of astaxanthin against contrast-induced acute kidney injury via SIRT1-p53 pathway in rats. Int Urol Nephrol. Google Scholar

Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  • Osman Sutcuoglu
    • 1
  • Mehmet Kursat Derici
    • 2
    Email author
  • Ozge Tugce Pasaoglu
    • 3
  • Burak Dumludag
    • 4
  • Ozant Helvacı
    • 1
  • Betul Ogut
    • 5
  • Ipek Isık Gonul
    • 5
  • Ulver Derici
    • 1
  1. 1.Department of NephrologyGazi University Faculty of MedicineAnkaraTurkey
  2. 2.Department of Medical PharmacologyKirikkale University Faculty of MedicineYahşihan/KırıkkaleTurkey
  3. 3.Department of BiochemistryGazi University Faculty of MedicineAnkaraTurkey
  4. 4.Department of Internal MedicineHınıs Sehit Yavuz Yurekseven HospitalErzurumTurkey
  5. 5.Department of PathologyGazi University Faculty of MedicineAnkaraTurkey

Personalised recommendations