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MicroRNA-15a expression measured in urine samples as a potential biomarker of renal cell carcinoma

  • Urology - Original Paper
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Abstract

Introduction

Currently, there is no accurate diagnostic molecular biomarker for renal cell carcinoma (RCC). The aim of this study was to assess the expression of microRNA-15a (miR-15a) in urine of patients with RCC and to evaluate its potential as a diagnostic molecular biomarker.

Materials and methods

In total, 67 patients with solid renal tumors were enrolled: clear-cell RCC (ccRCC, n = 22), papillary RCC (pRCC, n = 16), chromophobe RCC (chRCC, n = 14), oncocytoma (n = 8), papillary adenoma (n = 2) and angiomyolipoma (n = 5). MiRNA-15a expression levels measurement in urine were performed using qPCR. Urine of 15 healthy volunteers without kidney pathology was used as control.

Results

We observed a difference in mean miR-15a expression values in groups of pre-operative patients with RCC, benign renal tumors and healthy persons (2.50E−01 ± 2.72E−01 vs 1.32E−03 ± 3.90E−03 vs 3.36E−07 ± 1.04E−07 RFU, respectively, p < 0.01). There was no difference in miR-15a expression between ccRCC, pRCC and chRCC (p > 0.05). Direct association between RCC size and miR-15a expression values was obtained (Pearson correlation coefficient—0.873). On the 8th day after nephrectomy, mean expression value in patients with RCC decreased by 99.53% (p < 0.01). MiR-15a expression differentiated RCC from benign renal tumors with 98.1% specificity, 100% sensitivity at a cut-off value of 5.00E−06 RFU, with AUC—0.955.

Conclusions

MiR-15a expression measured in urine may be used as diagnostic molecular biomarker for RCC.

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Acknowledgements

We are thankful to Prof. Anthony Zulli for the revision of English language.

Author information

Authors and Affiliations

  1. Department of Urology, Danylo Halytsky Lviv National Medical University, Pekarska Str. 69, Lviv, Ukraine

    Yulian Mytsyk & Yuriy Borys

  2. Department of General and Molecular Pathophysiology, Bogomoletz Institute of Physiology, National Academy of Sciences of Ukraine, Kiev, Ukraine

    Victor Dosenko

  3. Department of Radiology, Danylo Halytsky Lviv National Medical University, Lviv, Ukraine

    Askold Kucher

  4. Department of Nutrition, Faculty of Nursing and Professional Health Studies, Slovak Medical University, Limbova 12, 833 03, Bratislava, Slovak Republic

    Katarina Gazdikova

  5. Department of General Medicine, Faculty of Medicine, Slovak Medical University, Bratislava, Slovak Republic

    Katarina Gazdikova

  6. Weill Cornell Medical College in Qatar, Qatar Foundation, Education City, Doha, Qatar

    Dietrich Busselberg

  7. 1st Department of Internal Medicine, Faculty of Medicine, Comenius University and University Hospital, Bratislava, Slovakia

    Martin Caprnda

  8. Department of Internal Medicine, Brothers of Mercy Hospital, Brno, Czech Republic

    Peter Kruzliak

  9. Research and Development Services, Brno, Czech Republic

    Peter Kruzliak

  10. Laboratory for Translational Oncology and Personalized Medicine, Rashid Latif Medical College, Lahore, Pakistan

    Ammad Ahmad Farooqi

  11. Department of Foreign Languages, Danylo Halytsky Lviv National Medical University, Lviv, Ukraine

    Manyuk Lubov

  12. 2nd Department of Surgery, Faculty of Medicine, Masaryk University and St. Anne’s University Hospital, Pekarska 664/53, 656 91, Brno, Czech Republic

    Peter Kruzliak

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  1. Yulian Mytsyk
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Correspondence to Yulian Mytsyk, Katarina Gazdikova or Peter Kruzliak.

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All authors declare no conflict of interest.

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All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

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Informed consent was obtained from all individual participants included in the study.

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Mytsyk, Y., Dosenko, V., Borys, Y. et al. MicroRNA-15a expression measured in urine samples as a potential biomarker of renal cell carcinoma. Int Urol Nephrol 50, 851–859 (2018). https://doi.org/10.1007/s11255-018-1841-x

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  • DOI: https://doi.org/10.1007/s11255-018-1841-x

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